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  • Endo-blue method  (2)
  • High-temperature superconducting thin film  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Cloning and sequence comparison ofAvaI andBsoBI restriction-modification systems (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 695-699 
    Details
    ISSN: 1617-4623
    Keywords: Restriction enzyme ; Methylase selection ; Endo-blue method ; N4 methylase ; Inverse PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract AvaI andBsoBI restriction endonucleases are isoschizomers which recognize the symmetric sequence 5′CYCGRG3′ and cleave between the first C and second Y to generate a four-base 5′ extension. TheAvaI restriction endonuclease gene (avaIR) and methylase gene (avaIM) were cloned intoEscherichia coli by the methylase selection method. TheBsoBI restriction endonuclease gene (bsoBIR) and part of theBsoBI methylase gene (bsoBIM) were cloned by the “endo-blue” method (SOS induction assay), and the remainder ofbsoBIM was cloned by inverse PCR. The nucleotide sequences of the two restriction-modification (RM) systems were determined. Comparisons of the predicted amino acid sequences indicated thatAvaI andBsoBI endonucleases share 55% identity, whereas the two methylases share 41% identity. Although the two systems show similarity in protein sequence, their gene organization differs. TheavaIM gene precedesavaIR in theAvaI RM system, while thebsoBIR gene is located upstream ofbsoBIM in theBsoBI RM system. BothAvaI andBsoBI methylases contain motifs conserved among the N4 cytosine methylases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173975
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  • 2
    Electronic Resource
    Electronic Resource
    Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 695-699 
    Details
    ISSN: 1617-4623
    Keywords: Key words Restriction enzyme ; Methylase selection ; Endo-blue method ; N4 methylase ; Inverse PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  AvaI and BsoBI restriction endonucleases are isoschizomers which recognize the symmetric sequence 5′CYCGRG3′ and cleave between the first C and second Y to generate a four-base 5′ extension. The AvaI restriction endonuclease gene (avaIR) and methylase gene (avaIM) were cloned into Escherichia coli by the methylase selection method. The BsoBI restriction endonuclease gene (bsoBIR) and part of the BsoBI methylase gene (bsoBIM) were cloned by the “endo-blue” method (SOS induction assay), and the remainder of bsoBIM was cloned by inverse PCR. The nucleotide sequences of the two restriction-modification (RM) systems were determined. Comparisons of the predicted amino acid sequences indicated that AvaI and BsoBI endonucleases share 55% identity, whereas the two methylases share 41% identity. Although the two systems show similarity in protein sequence, their gene organization differs. The avaIM gene precedes avaIR in the AvaI RM system, while the bsoBIR gene is located upstream of bsoBIM in the BsoBI RM system. Both AvaI and BsoBI methylases contain motifs conserved among the N4 cytosine methylases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173975
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Preparation of large-area high-quality YBCO thin films by pulsed laser deposition with Si heater and composite scanning of laser and target (1993)
    Springer
    Journal of superconductivity 6 (1993), S. 335-337 
    Details
    ISSN: 1572-9605
    Keywords: High-temperature superconducting thin film ; pulsed laser ablation ; heater ; composite scanning ; large area
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract We report on the successful preparation of large-area high-quality YBa2Cu3O7 superconducting thin films by pulsed excimer laser ablation with a Si heater and composite scanning of laser beam and target. The Si heater, composite scanning of laser beam and target, and experiment results are described. The temperature variation of the Si heater was 〈 ±0.5% in a 45×40 mm2 area of 900–1000 °C. Films were deposited on LaAlO3 substrates 35 mm in diameter. The thin films exhibited a thickness variation of ±2.5%; the superconducting properties wereT c0=91×0.5 K andJ c= (3.3±0.7)×106 A/cm2 at 77 K and zero magnetic field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00617481
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  • 4
    Electronic Resource
    Electronic Resource
    Preparation and properties of YBa2Cu3O7 thin films on sapphire with Yttria-stabilized zirconia buffer layer (1994)
    Springer
    Journal of superconductivity 7 (1994), S. 693-696 
    Details
    ISSN: 1572-9605
    Keywords: High-temperature superconducting thin film ; pulsed laser ablation ; buffer layer ; sapphire substrate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract YBa2Cu3O7 superconducting thin films were deposited onR-plane sapphire substrates with Yttria-stabilized zirconia buffer layer by laser ablation. The structure of the films was characterized by X-ray diffraction. Superconducting transitional temperatures as high as ≥90 K and critical current densities up to 2.2×106 A/cm2 (77 K) were achieved. The results of Auger depth profile showed that no obvious diffusion occurred between the substrate and the YBa2Cu3O7 thin film.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00721649
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