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  • 1
    Electronic Resource
    Electronic Resource
    Intracellular salt and solute concentrations in Ectothiorhodospira marismortui: glycine betaine and Nα-carbamoyl glutamineamide as osmotic solutes (1991)
    Springer
    Archives of microbiology 156 (1991), S. 350-355 
    Details
    ISSN: 1432-072X
    Keywords: Ectothiorhodospira ; Halophilic ; Compatible solutes ; Glycine betaine ; Nα-carbamoyl glutamineamide ; Sucrose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ectothiorhodospira marismortui, a moderately halophilic purple sulfur bacterium from a hypersaline sulfur spring, contains glycine betaine and Nα-carbamoyl glutamineamide (CGA) as the main intracellular osmotic solutes, with sucrose as a minor component. The concentration of glycine betaine was found to increase with increasing salt concentration of the medium, from 0.47 M to 1.29 M in cells grown from 0.85 to 2.56 M NaCl, while the estimated CGA concentration rose from about 0.2 M to 0.5 M. The concentration of sucrose remained constant at a value of around 0.05 M. Intracellular sodium and potassium concentrations were relatively low (around 0.5 and 0.3 M, respectively, at an external NaCl concentration of 1.8 M). The concentration of the novel compound Nα-carbamoyl glutamineamide was enhanced when l-glutamine was added to the growth medium, suggesting that glutamine served as a precursor for the synthesis of the compound.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00248709
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  • 2
    Electronic Resource
    Electronic Resource
    Ectothiorhodospira marismortui sp. nov., an obligately anaerobic, moderately halophilic purple sulfur bacterium from a hypersaline sulfur spring on the shore of the Dead Sea (1989)
    Springer
    Archives of microbiology 151 (1989), S. 524-529 
    Details
    ISSN: 1432-072X
    Keywords: Ectothiorhodospira marismortui sp. nov. ; Purple sulfur bacteria ; Sulfide ; Photoorganotrophic ; Anaerobic ; Halophilic ; 16S rRNA oligonucleotide catalog
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel type of purple sulfur bacterium was isolated from a hypersaline sulfur spring on the shore of the Dead Sea. The cells of the isolate are irregularly rod-shaped or curved, and motile by means of a tuft of polar flagella. The photosynthetic system, containing bacteriochlorophyll a and carotenoids of the spirilloxanthin series, is located on stacks of lamellar membranes in the cell cytoplasm. The organism can grow either photoautotrophically with sulfide as electron donor, which is oxidized via extracellular sulfur to sulfate, or photoheterotrophically, using acetate, succinate, fumarate, malate or pyruvate as carbon sources. The bacterium is obligately anaerobic, and requires a source of reduced sulfur for growth. The isolate is moderately halophilic, and grows optimally at NaCl concentrations between 3 and 8%, temperatures between 30 and 45°C, and neutral pH. 16S ribosomal RNA oligonucleotide cataloging suggests a close relationship to purple sulfur bacteria of the genus Ectothiorhodospira. As the isolate differs greatly from the described members of the genus Ectothiorhodospira, we describe the isolate as a new species, and propose the name Ectothiorhodospira marismortui sp. nov.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00454869
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  • 3
    Electronic Resource
    Electronic Resource
    Occurrence of virus-like particles in the Dead Sea (1997)
    Springer
    Extremophiles 1 (1997), S. 143-149 
    Details
    ISSN: 1433-4909
    Keywords: Key words Virus-like particles ; Dead Sea ; Halophilic ; Archaea ; Hypersaline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Electron-microscopic examination of water samples from the hypersaline Dead Sea showed the presence of high numbers of virus-like particles. Between 0.9 and 7.3 × 107 virus-like particles ml−1 were enumerated in October 1994 in the upper 20 m of the water column during the decline of a bloom of halophilic Archaea. Virus-like particles outnumbered bacteria by a factor of 0.9–9.5 (average 4.4). A variety of viral morphologies were detected, the most often encountered being spindle-shaped, followed by polyhedral and tailed phages. In addition, other types of particles were frequently found, such as unidentified algal scales, and virus-sized star-shaped particles. Water samples collected during 1995 contained low numbers of both bacteria and virus-like particles (1.9–2.6 × 106 and 0.8–4.6 × 107 ml−1 in April 1995), with viral numbers sharply declining afterwards (less than 104 ml−1 in November 1995–January 1996). It is suggested that viruses may play a major role in the decline of halophilic archaeal communities in the Dead Sea, an environment in which protozoa and other predators are absent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007920050027
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