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  • 25S ribosomal RNA  (1)
  • HAP1, HAP2 transcription factors  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    The 5′-upstream region of the yeast 25S rRNA gene contains a promoter element allowing expression in yeast and E. coli (1988)
    Springer
    Current genetics 14 (1988), S. 293-302 
    Details
    ISSN: 1432-0983
    Keywords: 25S ribosomal RNA ; Pol II promoter structure ; lacZ fusions ; Heterologous expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 25S rRNA gene of Saccharomyces cerevisiae is preceded by a bona fide TATA sequence which allows the initiation of transcription — presumably by polymerase II — from the same strand as the 25S rRNA gene. When the promoter fragment is cloned in front of a lacZ gene equipped with an initiation codon but lacking a promoter, this element permits formation of β-galactosidase both in yeast and E. coli. Using RNA from yeast transformed with the fusion plasmid, we mapped by primer elongation a single initiation site 63 by downstream from the presumed TATA sequence, i.e. about 53 by 5′ of, the 25S rRNA gene. A similar signal at about the same position was observed when RNA from untransformed wild-type yeast was used as a template for primer elongation. These results suggest that transcription from this polymerase II promoter-like element occurs in vivo. A regulatory function could not be assigned to this transcript. Its initiation is not significantly influenced by heme or carbon source, although two boxes of high homology with upstream activation sequences (UAS) mediating heme dependent expression of the iso-1-cytochrome c gene (CYC1) precede the promoter at the appropriate distance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419985
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of yeast cytochrome C1 is controlled at the transcriptional level by glucose, oxygen and haem (1992)
    Springer
    Molecular genetics and genomics 232 (1992), S. 447-459 
    Details
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; Cytochrome c 1 ; Promoter dissection ; HAP1, HAP2 transcription factors ; Centromere and promoter-binding factor (CPF1)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nuclear gene for cytochrome c 1 in Saccharomyces cerevisiae (CYT1) was localized on chromosome XV. Its upstream region was identified by functional complementation. Fusion to the lacZ reporter gene on a CEN plasmid allowed study of the effect of carbon sources and of specific deletion mutations on expression of the gene in yeast transformants. Detailed promoter analysis combined with expression studies in recipient strains defective in regulatory genes identified cis-acting sites and transcription factors involved in the regulated expression of the cytochrome c 1 gene. These analyses showed that, in the presence of glucose, transcription of CYT1 is positively controlled by oxygen, presumably through the haem signal, and mediated by the HAP1-encoded transactivator. It is additionally regulated by the HAP2/3/4 complex which mediates gene activation mainly under glucose-free conditions. Basal transcription is, in part, effected by CPF1, a centromere and promoter-binding factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266250
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    Paper (German National Licenses)
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