ISSN:
1432-072X
Keywords:
Bacillus subtilis
;
Growth rate
;
Guanosine triphosphate
;
RNA accumulation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract After addition of amino acids to a Bacillus subtilis glucose culture the intracellular guanosine triphosphate (GTP) concentration increased. The growth rate and the rate of RNA accumulation increased too. With mycophenolic acid, an inhibitor of inosinate dehydrogenase, it was possible to adjust the intracellular GTP concentration to values ranging between that of the glucose culture and that of the culture which had received amino acids. This led to intermediate growth rate values. Guanosine abolished the mycophenolic acid inhibition of GTP synthesis. It also counteracted the drug effects on growth rate and RNA accumulation. In cultures growing on Nutrient Sporulation Medium, in which the growth rate decreases as cell density increases, the GTP concentration did correlate with the growth rate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00405887
Permalink
