ISSN:
1617-4623
Keywords:
Chlamydomonas reinhardtii
;
Sexual differentiation
;
Gamete-specific genes
;
Gene expression patterns
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Four genes specifically expressed during gametogenesis of Chlamydomonas reinhardtii have been cloned and their expression patterns analyzed. mRNAs encoded by these gamete-specific genes (gas) were absent or present only at very low levels in vegetative cells and mature zygotes. In young zygotes 2 h after gamete fusion, the mRNAs of three gas genes still persisted. The gas mRNAs accumulated during gametic differentiation. The temporal patterns of accumulation of individual mRNAs differed; some started to increase early during gametogenesis, others accumulated in the late phase. The accumulation of one of the late mRNAs (gas28) was stricly light-dependent. To illustrate the utility of the genes cloned in the analysis of sexual differentiation in Chlamydomonas reinhardtii we show that in a gametogenesis-defective mutant, the expression of late genes is prevented while that of early genes is normal.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00284695
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