ISSN:
1432-0878
Keywords:
Key words: Vacuolar ATPase
;
Proton pump
;
Electrogenic potassium transport
;
Malpighian tubules
;
Immunocytochemistry
;
Formica polyctena (Insecta)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract. The presence of a vacuolar-type ATPase in Malpighian tubules of the ant Formica polyctena was investigated immunocytochemically, using antibodies to vacuolar ATPases of Manduca sexta midgut and bovine kidney. Specific labelling was observed at the brush border of the epithelium, extending along the entire length of the tubules. These findings agree with the current view that a vacuolar ATPase is situated at the apical membrane of Malpighian tubule cells and other insect epithelial cells, being the energizing element of an electrogenic potassium pump. When antibodies were tested on tubules in different secretion conditions prior to fixation, no differences were observed in the distribution of the vacuolar ATPase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004410050485
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