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Immunocytochemical localization of a vacuolar-type ATPase in Malpighian tubules of the ant Formica polyctena

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Abstract

The presence of a vacuolar-type ATPase in Malpighian tubules of the ant Formica polyctena was investigated immunocytochemically, using antibodies to vacuolar ATPases of Manduca sexta midgut and bovine kidney. Specific labelling was observed at the brush border of the epithelium extending along the entire length of the tubules. These findings agree with the current view that a vacuolar ATPase is situated at the apical membrane of Malpighian tubule cells and other insect epithelial cells, being the energizing element of an electrogenic potassium pump. When antibodies were tested on tubules in different secretion conditions prior to fixation, no differences were observed in the distribution of the vacuolar ATPase.

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Authors and Affiliations

  1. Departmento de Histología y Anatomía Patológica, Edificio de Investigación, Universidad de Navarra, E-31080, Pamplona, Spain

    M. Garayoa, A. C. Villaro, L. M. Montuenga & P. Sesma

  2. Zoologisches Institut der Universität, Luisenstrasse14, D-80333, München, Germany

    U. Klein

  3. Institut für Zoologie der Universität, Universitätsstrasse 31, D-93040, Regensburg, Germany

    B. Zimmermann

Authors
  1. M. Garayoa
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  2. A. C. Villaro
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  3. U. Klein
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  4. B. Zimmermann
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  5. L. M. Montuenga
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  6. P. Sesma
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Additional information

This work was supported by grants from the European Community (SCI-CT90-0480), from the Ministerio de Educación y Ciencia DGICYT, Spain (CE 91-0002), and from the Deutsche Forschungsgemeinschaft (Wi 698-3).

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Garayoa, M., Villaro, A.C., Klein, U. et al. Immunocytochemical localization of a vacuolar-type ATPase in Malpighian tubules of the ant Formica polyctena . Cell Tissue Res 282, 343–350 (1995). https://doi.org/10.1007/BF00319124

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  • DOI: https://doi.org/10.1007/BF00319124

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