ISSN:
1432-072X
Schlagwort(e):
Ethanol tolerance
;
Cytoplasmic inheritance
;
Mitochondria
;
Growth rate
;
Fermentation rate
;
Ethanol inhibition constant
;
Aerobiosis
;
Anaerobiosis
;
S. cerevisiae
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract The presence of active mitochondria and oxidative metabolism is shown to be essential to maintain low inhibition levels by ethanol of the growth rate (μ), fermentation rate (v) or respiration rate (ϱ) of Saccharomyces cerevisiae wild type strain S288C. Cells which have respiratory metabolism show K i (ethanol inhibition constant) values for μ, v and ϱ, higher (K i〉1 M) than those of “petite” mutants or “grande” strains grown in anaerobiosis (K i=0.7 M). In addition, the relationship between μ or v and ethanol concentration is linear in cells with respiratory metabolism and exponential in cells lacking respiration. When functional mitochondria are transferred to “petite” mutants, the resulting strain shows K i values similar to those of the “grande” strain and the inhibition of μ and v by increasing ethanol concentrations becomes linear.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00491909
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