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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 190 (1978), S. 223-233 
    ISSN: 1432-0878
    Keywords: Oral epithelium ; Epithelial differentiation ; Stereology ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The epithelium of intact guinea pig palate was subjected to stereologic analysis in a study of structural alterations in the keratinizing epithelium in response to wounding. Point counting procedures were employed to analyse electron micrographs sampled from three epithelial strata in biopsies collected from five animals. The differentiation pattern of the guinea pig palate epithelium displayed the following structural density gradients from basal to granular layers: descending gradients of metabolically active organelles, ascending gradient of bundled filaments coupled with the appearence of membrane coating granules and keratohyalin granules, and a plateau-like gradient of cytoplasmic ground substance. This pattern of epithelial differentiation is basically identical to that of human hard palate epithelium and epidermis. Regional and species variations in structure of keratinizing epithelia are suggested based on interepithelial differences in morphometric parameters.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 177 (1977), S. 383-405 
    ISSN: 1432-0878
    Keywords: Oral epithelium ; Epithelial differentiation ; Filament function ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Epithel der normalen menschlichen Wangenschleimhaut wurde einer stereologischen Analyse unterworfen. 20 Biopsien aus 10–15 Jahre alten, gesunden Mädchen wurden für lichtund elektronenmikroskopische Studien verarbeitet. Aus 10 zufällig gewählten Biopsien wurden auf zwei Vergrößerungsstufen Stichproben elektronenmikroskopischer Bilder a) aus 4 Schichten im Bereich epithelialer Leisten und b) aus 3 Schichten im Epithel über Bindegewebspapillen entnommen. Insgesamt wurden 1820 Bilder mit Hilfe stereologischer Punktzählverfahren analysiert. Das Wangenepithel war im Durchschnitt 0,48 mm dick; es wurde von langen, schmalen Bindegewebspapillen durchzogen und umfaßte ein schmales, basales und suprabasales, sowie ein breites, homogen strukturiertes Oberflächenkompartment, gebildet aus dem Stratum spinosum und den Oberflächenschichten. Die vom Stratum basale gegen die Oberfläche fallenden oder steigenden Strukturgradienten ergaben ein epitheliales Differenzierungsmuster, das völlig verschieden von dem keratinisierender Epithelien ist. Dieses Muster wurde zur Hauptsache durch einen sehr starken Anstieg der Dichte der Filamente, die einen konstanten Durchmesser von 80 Å aufwiesen, durch einen entsprechenden Abfall der Volumendichte der zytoplasmatischen Grundsubstanz, durch das Auftreten von membranversteifenden Granula und durch individuell verschieden starke Glykogenablagerungen hervorgerufen. Es ist möglich, daß das dichte, filamentöse Maschenwerk, welches 70% des epithelialen Zytoplasma innerhalb einer breiten, oberflächlichen Epithelschicht füllt, als funktionelle Matrix für die Dehnbarkeit des Wangenepithels dient.
    Notes: Summary The epithelium of normal human buccal mucosa was subjected to stereologic analysis. Ten biopsies were selected from a total of 20 specimens collected from 10 to 15 year old females, and processed for lightand electron microscopy. At two levels of magnification, electron micrographs were sampled from four strata in epithelial ridges and from three strata in regions over connective tissue papillae. Stereologic point counting based on a recently improved system for analyzing stratified epithelia was employed to analyze a total of 1820 electron micrographs. Buccal epithelium was found to be 0.48 mm thick, interdigitated by long, slender connective tissue papillae, and comprised of a narrow basal and suprabasal, and a broad, homogeneously structured spinous and surface compartment. From basal to surface layers, the epithelium displayed a differentiation pattern different from that of keratinizing epithelia. This pattern was a function mainly of a drastic density increase of cytoplasmic filaments of a constant 80 Å diameter, a corresponding decrease of the cytoplasmic ground substance, the appearance of dark-cored membrane coating granules and individually varying amounts of glycogen deposition. It is suggested that the dense meshwork of filaments which fill 70% of the epithelial cytoplasm in a broad subsurface and surface layer, serves as the functional matrix for epithelial distensibility.
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