Publication Date:
1999-08-28
Description:
The selenoprotein phospholipid hydroperoxide glutathione peroxidase (PHGPx) changes its physical characteristics and biological functions during sperm maturation. PHGPx exists as a soluble peroxidase in spermatids but persists in mature spermatozoa as an enzymatically inactive, oxidatively cross-linked, insoluble protein. In the midpiece of mature spermatozoa, PHGPx protein represents at least 50 percent of the capsule material that embeds the helix of mitochondria. The role of PHGPx as a structural protein may explain the mechanical instability of the mitochondrial midpiece that is observed in selenium deficiency.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ursini, F -- Heim, S -- Kiess, M -- Maiorino, M -- Roveri, A -- Wissing, J -- Flohe, L -- New York, N.Y. -- Science. 1999 Aug 27;285(5432):1393-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Dipartmento di Chimica Biologica, Universita di Padova, Viale G. Colombo 3, I-35121 Padova, Italy.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10464096" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Electrophoresis, Gel, Two-Dimensional
;
Electrophoresis, Polyacrylamide Gel
;
Glutathione Peroxidase/chemistry/isolation & purification/*physiology
;
Infertility, Male/metabolism
;
Male
;
Mitochondria/chemistry/enzymology
;
Oxidation-Reduction
;
Proteins/chemistry/isolation & purification/*physiology
;
Rats
;
Rats, Wistar
;
Selenium/deficiency/*physiology
;
Selenoproteins
;
Solubility
;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
;
Spermatids/chemistry/enzymology
;
*Spermatogenesis
;
Spermatozoa/chemistry/enzymology/*physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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