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  • 1
    Publication Date: 2011-01-19
    Description: The D2/AKT1/GSK-3β signaling pathway has been involved in the downstream intracellular effects of dopamine, in the pathophysiology of cognitive deficits and related brain activity in schizophrenia, as well as in response to treatment with antipsychotics. Polymorphisms in the D2 (DRD2 rs1076560) and AKT1 (AKT1 rs1130233) genes have been associated with their respective protein expression and with higher-order cognition and brain function, including attention. Given the strong potential for their relationship, we investigated the interaction of these polymorphisms on multiple molecular and in vivo phenotypes associated with this signaling pathway. We measured AKT1 and GSK-3β proteins and phosphorylation in human peripheral blood mononuclear cells, functional MRI cingulate response during attentional control, behavioral accuracy during sustained attention, and response to 8 wk of treatment with olanzapine in a total of 190 healthy subjects and 66 patients with schizophrenia. In healthy subjects, we found that the interaction between the T allele of DRD2 rs1076560 and the A allele of AKT1 rs1130233 was associated with reduced AKT1 protein levels and reduced phosphorylation of GSK-3β, as well as with altered cingulate response and reduced behavioral accuracy during attentional processing. On the other hand, interaction of these two alleles was associated with greater improvement of Positive and Negative Syndrome Scale scores in patients with schizophrenia after treatment with olanzapine. The present results indicate that these functional polymorphisms are epistatically associated with multiple phenotypes of relevance to schizophrenia. Our results also lend support to further investigation of this downstream molecular pathway in the etiology and treatment of this disorder.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 2
    Publication Date: 1999-08-28
    Description: The selenoprotein phospholipid hydroperoxide glutathione peroxidase (PHGPx) changes its physical characteristics and biological functions during sperm maturation. PHGPx exists as a soluble peroxidase in spermatids but persists in mature spermatozoa as an enzymatically inactive, oxidatively cross-linked, insoluble protein. In the midpiece of mature spermatozoa, PHGPx protein represents at least 50 percent of the capsule material that embeds the helix of mitochondria. The role of PHGPx as a structural protein may explain the mechanical instability of the mitochondrial midpiece that is observed in selenium deficiency.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ursini, F -- Heim, S -- Kiess, M -- Maiorino, M -- Roveri, A -- Wissing, J -- Flohe, L -- New York, N.Y. -- Science. 1999 Aug 27;285(5432):1393-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Dipartmento di Chimica Biologica, Universita di Padova, Viale G. Colombo 3, I-35121 Padova, Italy.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10464096" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Electrophoresis, Gel, Two-Dimensional ; Electrophoresis, Polyacrylamide Gel ; Glutathione Peroxidase/chemistry/isolation & purification/*physiology ; Infertility, Male/metabolism ; Male ; Mitochondria/chemistry/enzymology ; Oxidation-Reduction ; Proteins/chemistry/isolation & purification/*physiology ; Rats ; Rats, Wistar ; Selenium/deficiency/*physiology ; Selenoproteins ; Solubility ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Spermatids/chemistry/enzymology ; *Spermatogenesis ; Spermatozoa/chemistry/enzymology/*physiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
    Publication Date: 2014-07-06
    Description: Supermassive black holes in the nuclei of active galaxies expel large amounts of matter through powerful winds of ionized gas. The archetypal active galaxy NGC 5548 has been studied for decades, and high-resolution x-ray and ultraviolet (UV) observations have previously shown a persistent ionized outflow. An observing campaign in 2013 with six space observatories shows the nucleus to be obscured by a long-lasting, clumpy stream of ionized gas not seen before. It blocks 90% of the soft x-ray emission and causes simultaneous deep, broad UV absorption troughs. The outflow velocities of this gas are up to five times faster than those in the persistent outflow, and, at a distance of only a few light days from the nucleus, it may likely originate from the accretion disk.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kaastra, J S -- Kriss, G A -- Cappi, M -- Mehdipour, M -- Petrucci, P-O -- Steenbrugge, K C -- Arav, N -- Behar, E -- Bianchi, S -- Boissay, R -- Branduardi-Raymont, G -- Chamberlain, C -- Costantini, E -- Ely, J C -- Ebrero, J -- Di Gesu, L -- Harrison, F A -- Kaspi, S -- Malzac, J -- De Marco, B -- Matt, G -- Nandra, K -- Paltani, S -- Person, R -- Peterson, B M -- Pinto, C -- Ponti, G -- Pozo Nunez, F -- De Rosa, A -- Seta, H -- Ursini, F -- de Vries, C P -- Walton, D J -- Whewell, M -- New York, N.Y. -- Science. 2014 Jul 4;345(6192):64-8. doi: 10.1126/science.1253787. Epub 2014 Jun 19.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉SRON Netherlands Institute for Space Research, Sorbonnelaan 2, 3584 CA Utrecht, Netherlands. Department of Physics and Astronomy, Universiteit Utrecht, Post Office Box 80000, 3508 TA Utrecht, Netherlands. Leiden Observatory, Leiden University, Post Office Box 9513, 2300 RA Leiden, Netherlands. j.kaastra@sron.nl. ; Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, MD 21218, USA. Department of Physics and Astronomy, Johns Hopkins University, Baltimore, MD 21218, USA. ; Istituto Nazionale di Astrofisica (INAF)-Istituto di Astrofisica Spaziale e Fisica Cosmica (IASF) Bologna, Via Gobetti 101, I-40129 Bologna, Italy. ; SRON Netherlands Institute for Space Research, Sorbonnelaan 2, 3584 CA Utrecht, Netherlands. Mullard Space Science Laboratory, University College London, Holmbury St. Mary, Dorking, Surrey RH5 6NT, UK. ; Universite Grenoble Alpes, Institut de Planetologie et d'Astrophysique de Grenoble (IPAG), F-38000 Grenoble, France. CNRS, IPAG, F-38000 Grenoble, France. ; Instituto de Astronomia, Universidad Catolica del Norte, Avenida Angamos 0610, Casilla 1280, Antofagasta, Chile. Department of Physics, University of Oxford, Keble Road, Oxford OX1 3RH, UK. ; Department of Physics, Virginia Tech, Blacksburg, VA 24061, USA. ; Department of Physics, Technion-Israel Institute of Technology, 32000 Haifa, Israel. ; Dipartimento di Matematica e Fisica, Universita degli Studi Roma Tre, via della Vasca Navale 84, 00146 Roma, Italy. ; Department of Astronomy, University of Geneva, 16 Chemin d'Ecogia, 1290 Versoix, Switzerland. ; Mullard Space Science Laboratory, University College London, Holmbury St. Mary, Dorking, Surrey RH5 6NT, UK. ; SRON Netherlands Institute for Space Research, Sorbonnelaan 2, 3584 CA Utrecht, Netherlands. ; Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, MD 21218, USA. ; SRON Netherlands Institute for Space Research, Sorbonnelaan 2, 3584 CA Utrecht, Netherlands. European Space Astronomy Centre, Post Office Box 78, E-28691 Villanueva de la Canada, Madrid, Spain. ; Cahill Center for Astronomy and Astrophysics, California Institute of Technology, Pasadena, CA 91125, USA. ; Universite de Toulouse, Universite Paul Sabatier (UPS)-Observatoire Midi-Pyrenees (OMP), Institut de Recherche en Astrophysique et Planelogie (IRAP), Toulouse, France. CNRS, IRAP, 9 Avenue colonel Roche, BP 44346, 31028 Toulouse Cedex 4, France. ; Max-Planck-Institut fur extraterrestrische Physik, Giessenbachstrasse, D-85748 Garching, Germany. ; 22 Impasse du Bois Joli, 74410 St. Jorioz, France. ; Department of Astronomy, Ohio State University, 140 West 18th Avenue, Columbus, OH 43210, USA. Center for Cosmology and AstroParticle Physics, Ohio State University, 191 West Woodruff Avenue, Columbus, OH 43210, USA. ; Institute of Astronomy, University of Cambridge, Madingley Road, Cambridge CB3 0HA, UK. ; Astronomisches Institut, Ruhr-Universitat Bochum, Universitatsstrasse 150, 44801, Bochum, Germany. ; INAF/Istituto di Astrofisica e Planetologia Spaziali, Via Fosso del Cavaliere 100, I-00133 Roma, Italy. ; Research Center for Measurement in Advanced Science, Faculty of Science, Rikkyo University 3-34-1 Nishi-Ikebukuro, Toshima-ku, Tokyo, Japan. Department of Physics, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24994647" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
    ISSN: 0392-6737
    Keywords: Biophysical instrumentation and techniques
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Description / Table of Contents: Riassunto È stato usato un apparato di misurazione a un fotone per misurare la chemiluminescenza emessa durante la perossidazione dei fosfolipidi della membrana cellulare di un cuore di ratto isolato e perfuso sottoposto ad uno stress perossidativo. Lo stress perossidativo è stato ottenuto aggiungendo idroperossidi al liquido di perfusione. I differenti idroperossidi usati hanno prodotto tipiche evoluzioni temporali di chemi-luminescenza altamente riproducibili. La disponibilità di ossigeno influenza fortemente l'intensità e la cinetica di emissione della chemiluminescenza. Nell'àmbito di uno studio sull'effetto di differenti idroperossidi sul cuore di ratto isolato e perfuso, la misura della chemiluminescenza si è dimostrata un interessante e promettente strumento per il monitoraggio in continuo dei danni perossidativi nelle membrane biologiche. La sensibilità di questo metodo per la valutazione della perossidazione dei fosfolipidi delle biomembrane è circa dieci volte maggiore rispetto agli ordinari metodi di chimica analitica.
    Abstract: Резюме В этой статъе описывается аппаратура для ретистрации отделъных фотонов для непрерывного мониторнта улътраслабой хемилюминесценции, испус-каемой во время пероксидации фосфолипидов клеточной мембраны изолированного и перфузированного сердца крысы. В частности, аппаратура для ретистрации отделъ-ных фотонов исполъзуется для оценки напряжениь, индуцированных в сердце при добавлении тидроперекиси в пербузирующую жидкостъ. для этого исполъзуутся различные тидропероксиды, которые дают типичные временные эволюции улътса-слабой хемиюминесценции. Также получается, что наличие кислорода силъно влияет на интенсивиостъ и кинетику улътраслабои хемилюминесценции. Показывается, что улътраслабая хемилюминесценция представляет оченъ интересный и перспективный инструмент для мониторинга пероксидативных повреждений биологических мембран. Чувствителъностъ этого метода для пценки пероксидации босболинидов биомембран почти в десятъ раз выше, чем обычнын методов аналитической химии.
    Notes: Summary This paper describes the use of a single-photon counting apparatus (SPCA) for the continuous monitoring of the ultraweak chemiluminescence (UWC) emitted by isolated and perfused rat hearts and deriving from phospholipid peroxydation. Specifically the SPCA was used to assess the peroxidative stress induced in the heart by the addition of an hydroperoxide to the perfusing medium. To this purpose, different hydroperoxides were used and each one produced a tipical and highly reliable temporal evolution of UWC. It was also found that the oxygen availability strongly influences the intensity and the kinetic of the UWC emission. UWC was proved to be a very interesting and promising tool for monitoring the peroxidative damage of biological membranes, both because it makes a continuous assessment possible and because the sensitivity of this method is approximately ten times greater than usual analytical chemistry methods.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 63 (1998), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of carnosine on irradiation inactivation kinetics of Aeromonas hydrophilawas studied. In phosphate buffer, carnosine had no effect on survival of the pathogen, whereas in turkey meat supplemented with carnosine the adverse effect of irradiation on A. hydrophila was reduced. Peroxy radicals were toxic to A. hydrophila, and this effect was counteracted by carnosine. Accordingly, in a kinetic test carnosine was capable of scavenging peroxy radicals. These results indicate that a free radical scavenger antioxidant such as carnosine can increase the radiation resistance of A. hydrophila in meat due to the inhibition of lipid peroxidation caused by radiolysis.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Archives of Biochemistry and Biophysics 297 (1992), S. 265-270 
    ISSN: 0003-9861
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
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  • 7
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Archives of Biochemistry and Biophysics 290 (1991), S. 458-462 
    ISSN: 0003-9861
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
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  • 8
    ISSN: 0003-9861
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Archives of Biochemistry and Biophysics 299 (1992), S. 330-333 
    ISSN: 0003-9861
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Archives of Biochemistry and Biophysics 295 (1992), S. 404-409 
    ISSN: 0003-9861
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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