ISSN:
1615-6102
Keywords:
Chromatin structure
;
DAPI
;
DNase I
;
Flow cytometry
;
Pea arbuscular mycorrhizas
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Lincoln and Frisson varieties of endomycorrhiza-forming pea plants and isogenic mycorrhiza-resistant Frisson mutant (P2) plants were inoculated withGlomus mosseae. Nuclei released from inoculated and non-inoculated (control) roots were analysed for chromatin structure and activity using flow cytometric techniques. Chromatin accessibility to the specific DNA fluorochrome DAPI at saturating and non-saturating concentrations was measured. DNA fluorescence of nuclei of mycorrhizal Lincoln and wild genotype Frisson plants was significantly increased, compared to the controls, at saturating and, more strongly, at non-saturating DAPI concentrations. In contrast, the nuclei of inoculated P2 mutant roots showed a much lower increase in fluorescence, compared to uninoculated controls. Nuclei released from mycorrhiza-infected Lincoln roots were more sensitive to DNase I than those of uninfected ones. These results indicate a dramatic increase in that portion of the genome which can be transcribed in response to AM infection.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01403279
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