Summary
Lincoln and Frisson varieties of endomycorrhiza-forming pea plants and isogenic mycorrhiza-resistant Frisson mutant (P2) plants were inoculated withGlomus mosseae. Nuclei released from inoculated and non-inoculated (control) roots were analysed for chromatin structure and activity using flow cytometric techniques. Chromatin accessibility to the specific DNA fluorochrome DAPI at saturating and non-saturating concentrations was measured. DNA fluorescence of nuclei of mycorrhizal Lincoln and wild genotype Frisson plants was significantly increased, compared to the controls, at saturating and, more strongly, at non-saturating DAPI concentrations. In contrast, the nuclei of inoculated P2 mutant roots showed a much lower increase in fluorescence, compared to uninoculated controls. Nuclei released from mycorrhiza-infected Lincoln roots were more sensitive to DNase I than those of uninfected ones. These results indicate a dramatic increase in that portion of the genome which can be transcribed in response to AM infection.
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Abbreviations
- AM:
-
arbuscular mycorrhizas
- CRBCs:
-
chicken red blood cells
- CV:
-
coefficient of variation
- DAPI 4′:
-
6-diamidino-2-phenylindole
- DNase I:
-
deoxyribonuclease I
- EDTA:
-
ethylenediamine tetraacetic acid
- FCM:
-
flow cytometry
- TMN:
-
Tris MgCl2 NaCl buffer
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Sgorbati, S., Berta, G., Trotta, A. et al. Chromatin structure variation in successful and unsuccessful arbuscular mycorrhizas of pea. Protoplasma 175, 1–8 (1993). https://doi.org/10.1007/BF01403279
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DOI: https://doi.org/10.1007/BF01403279