ISSN:
1432-2048
Keywords:
Crown-gall
;
Cytokinin in crown-gall
;
Mass spectrometry (cytokinins)
;
Nicotiana (cytokinins)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract the cytokinins of tobacco crown-gall tissue have been analysed by quantitative mass spectrometry using 2H2-labelled cytokinin riboside 5′-monophosphates and 15N4-labelled cytokinin glycosides as internal standards. The principal endogenous cytokinin of this tissue is zeatin riboside 5′-monophosphate. The biologically inactive 7-glucoside of zeatin is the most abundant basic cytokinin in the tissue. These findings expose the limitations of previously reported analyses of similar tissues, which were restricted to biologically active basic cytokinins. The present study demonstrates that the endogenous cytokinins of tobacco crowngall tissue show a clear correspondence to the range of metabolites formed when exogenous cytokinins are supplied to nontumorous tobacco cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00398725
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