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Mass-spectrometric quantification of cytokinin nucleotides and glycosides in tobacco crown-gall tissue

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Abstract

the cytokinins of tobacco crown-gall tissue have been analysed by quantitative mass spectrometry using 2H2-labelled cytokinin riboside 5′-monophosphates and 15N4-labelled cytokinin glycosides as internal standards. The principal endogenous cytokinin of this tissue is zeatin riboside 5′-monophosphate. The biologically inactive 7-glucoside of zeatin is the most abundant basic cytokinin in the tissue. These findings expose the limitations of previously reported analyses of similar tissues, which were restricted to biologically active basic cytokinins. The present study demonstrates that the endogenous cytokinins of tobacco crowngall tissue show a clear correspondence to the range of metabolites formed when exogenous cytokinins are supplied to nontumorous tobacco cells.

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Abbreviations

DHZ:

dihydrozeatin

DHZ7G:

dihydrozeatin 7-glucoside

DHZMP:

dihydrozeatin 9-riboside 5′-monophosphate

DHZR:

dihydrozeatin 9-riboside

GC-MS:

coupled gas chromatography-mass spectrometry

HPLC:

high-performance liquid chromatography

Z7G:

zeatin 7-glucoside

Z9G:

zeatin 9-glucoside

ZOG:

zeatin O-glucoside

ZMP:

zeatin 9-riboside 5′-monophosphate

ZR:

zeatin 9-riboside

ZROG:

zeatin 9-riboside O-glucoside

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Authors and Affiliations

  1. Department of Botany and Microbiology, University College of Wales, SY23 3DA, Aberystwyth, Dyfed, UK

    Ian M. Scott & Roger Horgan

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  1. Ian M. Scott
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  2. Roger Horgan
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Scott, I.M., Horgan, R. Mass-spectrometric quantification of cytokinin nucleotides and glycosides in tobacco crown-gall tissue. Planta 161, 345–354 (1984). https://doi.org/10.1007/BF00398725

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  • DOI: https://doi.org/10.1007/BF00398725

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