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  • Cloning vectors  (1)
  • Copper  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Regulation of Saccharomyces cerevisiae catalase gene expression by copper (1993)
    Springer
    Current genetics 24 (1993), S. 388-393 
    Details
    ISSN: 1432-0983
    Keywords: Copper ; Catalase ; Metallothionein ; ACE1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Treatment of Saccharomyces cerevisiae cells with copper induces the activity of Cu/Zn superoxide dismutase (SOD) and catalase. To understand the level at which Cu regulates catalase, the expression of the S. cerevisiae CTA1 (encoding the peroxisomal catalase A) and CTT1 (encoding the cytosolic catalase T) genes was monitored as a function of Cu treatment. Copper was found to specifically induce transcription of CTT1, but not CTA1, mRNA. Moreover, genetic and biochemical studies demonstrate that this induction is independent of the ACE1 Cu trans-activator controlling the expression of yeast Cu/Zn SOD and metallothionein genes. Copper regulation of CTT1 thus appears to represent a novel metal regulatory pathway in S. cerevisiae cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351846
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  • 2
    Electronic Resource
    Electronic Resource
    Construction of a yeast plasmid cloning vector with high stability inSaccharomyces cerevisiae strains deficient in 2 µmDNA (1984)
    Springer
    Monatshefte für Chemie 115 (1984), S. 1229-1235 
    Details
    ISSN: 1434-4475
    Keywords: Cloning vectors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung PlasmidpCP1 wurde ausgehend von klonierterSaccharomyces cerevisiae-2 µmDNA und PlasmidpJDB207 konstruiert. Der Vektor enthält die vollständige, imFLP-Gen unterbrocheneB-Form der 2 µmDNA, vomEscherichia coli-Plasmidp AT153 abgeleiteteDNA und eine wenig aktive Variante desS. cerevisiae-LEU2-Gens. Der neue Vektor weist unter nichtselektiven Wachstumsbedingungen incir +- undcir 0-Stämmen eine niedrige Verlustrate auf und ist incir 0-Stämmen stabil gegen Umlagerungen. Seine Verwendbarkeit für die Umwandlung voncir +-Stämmen incir 0-Stämme durch Verdrängung endogener 2 µm-DNA wurde nachgewiesen.
    Notes: Abstract PlasmidpCP1 was constructed from cloned 2 µmDNA ofSaccharomyces cerevisiae and from plasmidpJDB207. VectorpCP1 contains the completeB form of 2 µmDNA interrupted in theFLP gene, together withDNA derived from theEscherichia coli plasmidpAT153 and a low expression variant of theS. cerevisiae LEU2 gene. The new vector is lost at a low frequency from yeastcir + orcir 0 strains under non-selective growth conditions and is stable against rearrangements incir 0 strains. Its usefulness for curingcir + strains from endogenous 2 µmDNA and for their conversion tocir 0 strains was demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00809354
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