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  • 1
    Electronic Resource
    Electronic Resource
    Effects of growth conditions on the oxygen equivalence of microbial cells (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 17 (1975), S. 859-872 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A natural microbial population of sewage origin was grown at four temperatures (10, 20, 30, 40°C) and five mean residence times (3, 6, 12, 18, 24 hr) in a continuous stirred tank reactor. Samples of cells were removed during steady-state operation and analyzed for chemical oxygen demand (COD), ash content, protein, carbohydrate, and nucleic acids. The oxygen equivalence (OE) of the cells was measured directly as g COD/g ash-free cells and was determined indirectly by calculating the calorific content from the macromolecular composition. The results indicate that OE is a function of growth conditions. Furthermore the COD test is a poor measureof OE because of a high level of variation associated with the test. Additional studies should be performed using bomb calorimetry to establish a quantitative relationship between growth conditions and OE.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260170607
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  • 2
    Electronic Resource
    Electronic Resource
    An assessment of the role of physiological adaptation in the transient response of bacterial cultures (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 1427-1444 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The RNA-limiting theory of transient response states that the primary physiological adaptation which occurs when microbial cultures are grown at specific rates less than their maximum is a decrease in the cellular level of RNA. It predicts that, as a result of this decrease, the response of the culture to a shift-up in growth rate will be limited by its RNA level. In order to test the RNA-limiting theory and to investigate the role physiological adaptation in transient response, experiments were performed in which steady-state chemostat cultures of Pseudomonasputida grown at various specific rates were transferred to batch reactors containing sufficient carbon source (L-lysine) and nutrients to remove all external growth restrictions. Samples were collected during the subsequent transient period for determination of the macromolecular composition and the maximum instantaneous oxygen uptake rate. The results indicated that, while decreases in the RNA level did significantly affect the nature of the transient response, other unidentified components varied with the steady-state specific growth rate at which the culture had been grown prior to the shift-up and that the levels of those components affected the nature of the subsequent transient response. This implies that the RNA-limiting theory is inadequate for describing the transient responses of cultures grown over a wide range of specific growth rates.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260240614
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of growth rate and influent substrate concentration on effluent quality from chemostats containing bacteria in pure and mixed culture (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 14 (1972), S. 391-410 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Studies were performed using pure cultures of A. acrogenes and E. coli and a heterogeneous microbial population growing in carbon-limited chemostats with glucose as the sole carbon and energy source. A two-level factorial experimental design was employed to test the hypothesis that the concentration of growth-limiting substrate in a chemostat is controlled by the growth rate alone and is independent of the concentration of substrate entering the reactor. The pure culture experiments showed that the conclusions depend upon the measurement employed for growth-limiting substrate. When the concentration of glucose was measured directly, the hypothesis was found to be true within the limits of the study (500-1500 mg/liter). However, if the chemical oxygen demand (COD) test was used as the measure of growth-limiting substrate the hypothesis was found to be false. When heterogeneous cultures were employed the hypothesis was false regardless of the technique used to measure the concentration of growth-limiting substrate. Nevertheless, it was possible to generate regression equations which described the interactions among influent COD, growth rate, and effluent COD with a high level of correlation.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260140310
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  • 4
    Electronic Resource
    Electronic Resource
    Biodegradation: Its measurement and microbiological basis (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 660-674 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260270516
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  • 5
    Electronic Resource
    Electronic Resource
    Dual substrate removal by an axenic bacterial culture (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 33 (1989), S. 327-337 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A pure bacterial culture capable of utilizing either L-lysine or 2-chlorophenol (2-CP) as sole carbon source was isolated and used in continuous culture experiments to determine its response to dual substrate limitation by those two compounds. Dilution rate and feed composition were each set at three levels in a two factorial experimental design. The total chemical oxygen demand (COD) of the feed was fixed at 225 mg/L and its composition was varied by changing the ratio of lysine to 2-CP. The effects of the two independent variables (dilution rate and feed composition) on the concentrations of cells, lysine, COD, and dissolved organic carbon (DOC) in the reactors were systematic whereas the effects on the 2-CP concentration were less predictable. The concentrations of the two substrates responded to the two independent variables in a complex interactive manner which is not explained by existing models for dual, substitutable substrates. Rather, the results suggested that the prediction of the fate of a single organic component in a reactor receiving a multicomponent feed is a very difficult task.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260330312
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