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  • Chemistry  (2)
  • 1995-1999  (2)
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  • 1995-1999  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Tension and mobility of a DNA fragment in the lakes-straits model (1995)
    Weinheim : Wiley-Blackwell
    Macromolecular Theory and Simulations 4 (1995), S. 145-154 
    Details
    ISSN: 1022-1344
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: We consider numerically, in the framework of the lakes-straits model, the mobility of a DNA chain under field-inversion gel-electrophoresis (FIGE). Here we investigate the role of the gel's structure on the mobility. We consider two situations: (i) a DNA chain fragment is bounded by two straits (narrow gateways) at a fixed distance, but is otherwise free, and (ii) the fragment is, in addition, confined with in a closed volume (pore). We calculate the tension on the DNA fragment in the two cases. For Gaussian chains we evaluate the corresponding statistical weights exactly, using a cellular automation algorithm. We find that the resulting tension differs drastically in the two models considered. Nonetheless this difference influences only weakly the overall FIGE mobility.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mats.1995.040040110
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  • 2
    Electronic Resource
    Electronic Resource
    Statistical features in the lakes-straits model and the influence of hernias (1996)
    Weinheim : Wiley-Blackwell
    Electrophoresis 17 (1996), S. 1060-1064 
    Details
    ISSN: 0173-0835
    Keywords: Field inversion gel electrophoresis ; Lakes-straits model ; Hernias ; Mobility ; DNA chains ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: We evaluate numerically the mobility of DNA chains under field-inversion gel electrophoresis (FIGE) conditions in the framework of the lakes-straits model introduced by Zimm (Phys. Rev. Lett. 1988, 61, 2965-2968; J. Phys. Chem. 1991, 94, 2197-2206). We extend the model by allowing both simple and also multiple-branched hernias; this is achieved by arranging the data structure used in the algorithm so that each fragment in a lake can be treated separately. We show that the existence of hernias allows the probe to migrate faster and that with hernias the mobility minimum in FIGE shifts to smaller field periods. These effects occur only if the electric field is strong enough. We also discuss the influence of the model's parameters on the mobility.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150170614
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