Publication Date:
2009-03-31
Description:
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while simultanously generating villus-like epithelial domains in which all differentiated cell types are present. Single sorted Lgr5(+) stem cells can also initiate these cryptvillus organoids. Tracing experiments indicate that the Lgr5(+) stem-cell hierarchy is maintained in organoids. We conclude that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sato, Toshiro -- Vries, Robert G -- Snippert, Hugo J -- van de Wetering, Marc -- Barker, Nick -- Stange, Daniel E -- van Es, Johan H -- Abo, Arie -- Kujala, Pekka -- Peters, Peter J -- Clevers, Hans -- England -- Nature. 2009 May 14;459(7244):262-5. doi: 10.1038/nature07935. Epub 2009 Mar 29.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19329995" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Culture Techniques/*methods
;
Cell Lineage
;
Cell Separation
;
Gene Expression Regulation, Developmental
;
Intestines/*anatomy & histology/*cytology/metabolism
;
Mesoderm/cytology/metabolism
;
Mice
;
Multipotent Stem Cells/cytology/metabolism
;
Organoids/*cytology/growth & development/metabolism
;
Paneth Cells/metabolism
;
Receptors, G-Protein-Coupled/*metabolism
;
Receptors, Notch/metabolism
;
Regeneration
;
Stem Cell Niche
;
Stem Cells/*cytology/*metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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