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  • 1
    Digitale Medien
    Digitale Medien
    ConA induced changes in energy metabolism of rat thymocytes (1992)
    Springer
    Bioscience reports 12 (1992), S. 109-114 
    Details
    ISSN: 1573-4935
    Schlagwort(e): energy metabolism ; oxygen consumption ; thymocytes ; ConA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The influence of ConA on the energy metabolism of quiescent rat thymocytes was investigated by measuring the effects of inhibitors of protein synthesis, proteolysis, RNA/DNA synthesis, Na+K+-ATPase, Ca2+-ATPase and mitochondrial ATP synthesis on respiration. Only about 50% of the coupled oxygen consumption of quiescent thymocytes could be assigned to specific processes using two different media. Under these conditions the oxygen is mainly used to drive mitochondrial proton leak and to provide ATP for protein synthesis and cation transport, whereas oxygen consumption to provide ATP for RNA/DNA synthesis and ATP-dependent proteolysis was not measurable. The mitogen ConA produced a persistent increase in oxygen consumption by about 30% within seconds. After stimulation more than 80% of respiration could be assigned to specific processes. The major oxygen consuming processes of ConA-stimulated thymocytes are mitochondrial proton leak, protein synthesis and Na+K+-ATPase with about 20% each of total oxygen consumption, while Ca2+-ATPase and RNA/DNA synthesis contribute about 10% each. Quiescent thymocytes resemble resting hepatocytes in that most of the oxygen consumption remains unexplained. In contrast, the pattern of energy metabolism in stimulated thymocytes is similar to that described for Ehrlich Ascites tumour cells and splenocytes, which may also be in an activated state. Most of the oxygen consumption is accounted for, so the unexplained process(es) in unstimulated cells shut(s) off on stimulation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02351215
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    ConA induced changes in energy metabolism of rat thymocytes (1992)
    Springer
    Bioscience reports 12 (1992), S. 381-386 
    Details
    ISSN: 1573-4935
    Schlagwort(e): energy metabolism ; oxygen consumption ; thymocytes ; ConA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The influence of ConA on the energy metabolism of quiescent rat thymocytes was investigated by measuring the effects of inhibitors of protein synthesis, proteolysis, RNA/DNA synthesis, Na+K+-ATPase, Ca2+-ATPase and mitochondrial ATP synthesis on respiration. Only about 50% of the coupled oxygen consumption of quiescent thymocytes could be assigned to specific processes using two different media. Under these conditions the oxygen is mainly used to drive mitochondrial proton leak and to provide ATP for protein synthesis and cation transport, whereas oxygen consumption to provide ATP for RNA/DNA synthesis and ATP-dependent proteolysis was not measurable. The mitogen ConA produced a persistent increase in oxygen consumption by about 30% within seconds. After stimulation more than 80% of respiration could be assigned to specific processes. The major oxygen consuming processes of ConA-stimulated thymocytes are mitochondrial proton leak, protein synthesis and Na+K+-ATPase with about 20% each of total oxygen consumption, while Ca2+-ATPase and RNA/DNA synthesis contribute about 10% each. Quiescent thymocytes resemble resting hepatocytes in that most of the oxygen consumption remains unexplained. In constrast, the pattern of energy metabolism in stimulated thymocytes is similar to that described for Ehrlich Ascites tumour cells and splenocytes, which may also be in an activated state. Most of the oxygen consumption is accounted for, so the unexplained process(es) in unstimulated cells shut(s) off on stimulation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01121501
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 3
    Digitale Medien
    Digitale Medien
    Cytophysiology of neurosecretory axon terminals in the brain of an annelid (Ophryotrocha puerilis, Polychaeta) (1991)
    Springer
    Cell & tissue research 264 (1991), S. 339-345 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Neurosecretion ; Catecholamines ; HPLC ; Immunohistochemistry ; Glyoxylic acid fluorescence ; Ophryotrocha puerilis (Annelida)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In the posterior part of the brain of the protandric polychaete Ophryotrocha puerilis neurosecretory cells form prominent axon terminals. The terminals are arranged in two complexes. The perikarya of these presumably monopolar neurons are scattered in the anterior part of the cerebral perikaryal layer. In females the terminals store large amounts of neurosecretory material. It has been suggested earlier that neurosecretions of the terminals may play a role during sex reversal from females to males. Application of histamine caused the release of neurosecretory material from the respective terminals in females. However, this discharge was not followed by sex reversal. Application of reserpine had no influence on the terminals. Neither by in vivo observation nor by ultrastructural analysis any effect of reserpine on the terminal complexes could be observed. In isolated terminals filled with neurosecretory material from females, catecholamines could not be detected by HPLC. Also, polyclonal antibodies against dopamine did not stain the terminal complexes. Furthermore, the complexes did not develop any fluorescence after glyoxylic acid treatment. Therefore, the present results contradict the hypothesis that the neurosecretory material of the respective axon terminals is catecholaminergic and that it is involved in sex differentiation. The function of the secretory neurons studied here remains unclear.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00313972
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    The catecholaminergic system of an annelid (Ophryotrocha puerilis, Polychaeta) (1991)
    Springer
    Cell & tissue research 265 (1991), S. 175-184 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Nervous system ; Nervous system, peripheral ; Catecholamines ; Immunohistochemistry ; Glyoxylic acid fluorescence ; Ophryotrocha puerilis (Annelida)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The complex catecholaminergic (CA) nervous system of the polychaete Ophryotrocha puerilis is documented using glyoxylic acid induced fluorescence (GIF) and immunohistochemistry. CA-neurons are found both in the central and peripheral nervous system. In the brain, about 50 CA-neurons are present in the perikaryal layer together with numerous CA fibres. Two pairs of CA perikarya are characteristic for each ganglion of the ventral nerve cord. CA-neurites in the ventral nerve cord are mainly arranged in 4 strands paralleling the longitudinal axis of the worm. Fluorescent neurons with receptive ciliary structures are present in body appendages (antennae, palps, urites, parapodial cirri), in the body-wall, and within the oesophageal wall. Furthermore, a subepidermal nerve net of free CA nerve endings has been found. After incubation of specimens with dopamine prior to the development of GIF more fluorescent perikarya could be observed; the fluorescence was also intensified. Pre-incubation with reserpine reduced the intensity of GIF. Results of high pressure liquid chromatography and immunostaining with a polyclonal antibody against a dopamine-glutaraldehyde-complex suggest that dopamine is the major CA transmitter. It is thought that dopaminergic neurons together with ciliary receptive structures act as mechano- and/or chemoreceptors.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318152
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
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  • 5
    facet.materialart.
    Unbekannt
    Prompt neutron polarization asymmetries in photofission of Th232, U233,235,238, Np237, and Pu239,240 (2014)
    American Physical Society (APS)
    Details
    Publikationsdatum: 2014-03-20
    Beschreibung: Author(s): J. M. Mueller, M. W. Ahmed, R. H. France, III, M. S. Johnson, H. J. Karwowski, L. S. Myers, J. Randrup, M. H. Sikora, M. C. Spraker, S. Stave, J. R. Tompkins, R. Vogt, H. R. Weller, C. S. Whisnant, and W. R. Zimmerman Photofission experiments were performed on targets of Th232, U233,235,238, Np237, and Pu239,240 using nearly 100% linearly polarized, high intensity (∼5×106γ/s), and nearly-monoenergetic γ-ray beams having energies between 5.3 and 7.6 MeV at the High Intensity γ-ray Source (HIγS) located at Duke Uni... [Phys. Rev. C 89, 034615] Published Wed Mar 19, 2014
    Schlagwort(e): Nuclear Reactions
    Print ISSN: 0556-2813
    Digitale ISSN: 1089-490X
    Thema: Physik
    Publiziert von American Physical Society (APS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    facet.materialart.
    Unbekannt
    Measurement of prompt neutron polarization asymmetries in photofission of ^{235,238}U, ^{239}Pu, and ^{232}Th (2012)
    American Physical Society (APS)
    Details
    Publikationsdatum: 2012-01-12
    Beschreibung: Author(s): J. M. Mueller, M. W. Ahmed, B. Davis, J. M. Hall, S. S. Henshaw, M. S. Johnson, H. J. Karwowski, D. Markoff, L. S. Myers, B. A. Perdue, S. Stave, J. R. Tompkins, M. J. Tuffley, and H. R. Weller [Phys. Rev. C 85, 014605] Published Wed Jan 11, 2012
    Schlagwort(e): Nuclear Reactions
    Print ISSN: 0556-2813
    Digitale ISSN: 1089-490X
    Thema: Physik
    Publiziert von American Physical Society (APS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    facet.materialart.
    Unbekannt
    Compton scattering from Li6 at 86 MeV (2014)
    American Physical Society (APS)
    Details
    Publikationsdatum: 2014-08-27
    Beschreibung: Author(s): L. S. Myers, M. W. Ahmed, G. Feldman, A. Kafkarkou, D. P. Kendellen, I. Mazumdar, J. M. Mueller, M. H. Sikora, H. R. Weller, and W. R. Zimmerman Cross sections for Li6(γ,γ)Li6 have been measured at the High Intensity Gamma-Ray Source (HIγ⃗S) and the sensitivity of these cross sections to the nucleon isoscalar polarizabilities was studied. Data were collected using a quasimonoenergetic 86-MeV photon beam at photon scattering angles of 40∘–160... [Phys. Rev. C 90, 027603] Published Tue Aug 26, 2014
    Schlagwort(e): Nuclear Reactions
    Print ISSN: 0556-2813
    Digitale ISSN: 1089-490X
    Thema: Physik
    Publiziert von American Physical Society (APS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    facet.materialart.
    Unbekannt
    Polarization asymmetries in the ^{9} Be(γ,n_{0} ) reaction (2015)
    American Physical Society (APS)
    Details
    Publikationsdatum: 2015-09-11
    Beschreibung: Author(s): J. M. Mueller, M. W. Ahmed, B. J. Davis, H. J. Karwowski, D. M. Markoff, L. S. Myers, M. C. Spraker, S. Stave, J. R. Tompkins, H. R. Weller, and W. R. Zimmerman Measurements of the Be 9 ( γ , n 0 ) reaction were performed using nearly 100% linearly polarized, high-intensity, and nearly monoenergetic γ -ray beams having energies between 5.5 and 15.5 MeV at the High Intensity γ -ray Source located at Duke University and Triangle Universities Nuclear Laboratory. Eighte… [Phys. Rev. C 92, 034604] Published Thu Sep 10, 2015
    Schlagwort(e): Nuclear Reactions
    Print ISSN: 0556-2813
    Digitale ISSN: 1089-490X
    Thema: Physik
    Publiziert von American Physical Society (APS)
    Standort Signatur Erwartet Verfügbarkeit
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