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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 50 (1994), S. 148-152 
    ISSN: 1420-9071
    Keywords: Calliactis parasitica ; nematocytes ; discharge ; hyposmotic shock ; Gd3+
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract On acontia ofCalliactis parasitica it was observed that mechanical stimuli applied by a gelatin probe, a method effective in tentacles of Anthozoa, do not induce the discharge of nematocytes. Hyposmotic shock, performed by treatment with NaCl solution 35% hyposmotic with respect to sea water, induces, in the presence of Ca2+, the discharge that spreads along the acontial filament, as previously observed following treatment with SCN−. The hyposmotic shock-induced discharge is blocked by Gd3+ at a concentration of 1 μM. 10 μM Gd3+ prevents also the SCN−-induced discharge. These results suggest the presence of stretch activated cation channels either in nematocytes and/or in supporting cells as well as a possible effect of SCN− on this class of ion channels.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5117
    Keywords: Calliactis parasitica ; Aiptasia mutabilis ; anemones ; Anthozoa ; nematocysts ; discharge ; trypsin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Glycerol-isolated basitrichs of Calliactis parasitica responsive to thioglycolate had open apical flaps, while unresponsive capsules isolated with Triton X100 or by freezing had closed apical flaps. Limited treatment with trypsin induced the apical flaps to open without causing discharge, suggesting that nematocysts can maintain the resting condition even with open flaps. Trypsin-treated basitrichs acquire a high responsiveness to thioglycolate. Microbasic mastigophores of Aiptasia mutabilis are more responsive to distilled water after controlled trypsin treatment but the apical flaps are unchanged. Ca2+ is inhibitory regardless of trypsin treatment. It is proposed that the capsule tip may control the penetration of the discharging agents rather than providing mechanical resistance to inner pressure.
    Type of Medium: Electronic Resource
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