ISSN:
1573-4919
Keywords:
creatine kinase
;
arginine kinase
;
protein sequence comparison
;
evolution
;
CK framework
;
‘diagnostic boxes’
;
secondary structure prediction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Abstract Comparisons of the protein sequences and gene structures of the known creatine kinase isoenzymes and other guanidino kinases revealed high homology and were used to determine the evolutionary relationships of the various guamidino kinases. A ‘CK framework’ is defined, consisting of the most conserved sequence blocks, and ‘diagnostic boxes’ are identified which are characteristic for anyone creatine kinase isoenzyme (e.g. for vertebrate B-CK) and which may serve to distinguish this isoenzyme from all others (e.g. from M-CKs and Mi-CKs). Comparison of the guanidino kinases by near-UV and far-UV circular dichroism further indicates pronounced conservation of secondary structure as well as of aromatic amino acids that are involved in catalysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01267958
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