ISSN:
1617-4623
Keywords:
Brassica napus
;
Chloroplast genome
;
Anacystis nidulans
;
Cyanobacterial transformation
;
Plasmid stability
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Brassica napus (cv. “Triton”) chloroplast (cp) DNA BamHI gragments were inserted into a bacteria-cyanobacteria shuttle vector pCB4. The chloroplast genomic library was screened in Escherichia coli and 28 individual clones, which represent 94% of the total chloroplast genome, were isolated. Cyanobacterium Anacystis nidulans R2 was transformed with each member of the clone bank by selection for ampicillin resistance. A study of transformation efficiency showed dramatic variation (up to 200-fold) among recombinant clones. Furthermore, plasmid DNA reisolated from some cyanobacterial transformants exhibited instability. Variations in transformation efficiency and plasmid instability were shown to be DNA sequence specific. B. napus cpDNA clones were thus classified into three types according to their stability in the cyanobacterial host.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00337726
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