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  • 1
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    Simultaneous saccharification and fermentation of dilute alkaline-pretreated corn stover for enhanced butanol production by Clostridium saccharobutylicum DSM 13864 (2016)
    Oxford University Press
    Details
    Publication Date: 2016-02-03
    Description: Simultaneous saccharification and fermentation (SSF) process was applied for biobutanol production by Clostridium saccharobutylicum DSM 13864 from corn stover (CS). The key influential factors in SSF process, including corn steep liquor concentration, dry biomass and enzyme loading, SSF temperature, inoculation size and pre-hydrolysis time were optimized. In 5-L bioreactor with SSF process, butanol titer and productivity of 12.3 g/L and 0.257 g/L/h were achieved at 48 h, which were 20.6% and 21.2% higher than those in separate hydrolysis and fermentation (SHF), respectively. The butanol yield reached 0.175 g/g pretreated CS in SSF, representing 50.9% increase than that in SHF (0.116 g/g pretreated CS). This study proves the feasibility of efficient and economic production of biobutanol from CS by SSF.
    Keywords: Biotechnology & Synthetic Biology
    Print ISSN: 0378-1097
    Electronic ISSN: 1574-6968
    Topics: Biology
    Published by Oxford University Press
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  • 2
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    Construction and characterization of three protein-targeting expression system in Lactobacillus casei (2016)
    Oxford University Press
    Details
    Publication Date: 2016-03-13
    Description: We previously reported that the β-1,4-Mannanase ( manB ) gene from Bacillus pumilus functions as a good reporter gene in Lactobacillus casei . Two vectors were constructed. One carries the signal peptide of secretion protein Usp45 (SP Usp45 ) from Lactococcus lactis (pELSH), and the other carries the full-length S-layer protein, SlpA, from L. acidophilus (pELWH). In this work, another vector, pELSPH, was constructed to include the signal peptide of protein SlpA (SP SlpA ), and the capacity of all three vectors to drive expression of the manB gene in L. casei was evaluated. The results showed that SP Usp45 is functionally recognized and processed by the L. casei secretion machinery. The SP Usp45 -mediated secretion efficiency was ~87%, and SP SlpA drove the export of secreted ManB with ~80% efficiency. SP SlpA secretion was highly efficient, and expressed SlpA was anchored to the cell wall by an unknown secretion mechanism. Full-length SlpA drove the cell wall-anchored expression of an SlpA-ManB fusion protein but at a much lower level than that of protein SlpA.
    Keywords: Biotechnology & Synthetic Biology
    Print ISSN: 0378-1097
    Electronic ISSN: 1574-6968
    Topics: Biology
    Published by Oxford University Press
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  • 3
    Electronic Resource
    Electronic Resource
    On the computation of complex equilibria (1972)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 18 (1972), S. 299-304 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method is described for solving the problem: given fixed pressure, temperature, the amounts of the chemical elements, and the chemical potentials of possible chemical species at the pressure and temperature specified, determine the equilibrium composition. The method requires a minimum of judgment on the part of the user.A modification of Naphtali's method for direct minimization of Gibbs' function provides an estimate of the composition of sufficient accuracy to insure convergence of solution, by the Newton-Raphson method, of the nonlinear equations describing the equilibrium.Problems in treating multiple phaes of unknown stability and chemical species present in small amouts are handled by ignoring unstable phases and small quantitites in the direct minimization until the amounts of the major consitituents have been at least approximately determined. To accomplish this, species temporarily ignored are assigned a ficitious mol fraction so that their re-entry into the calculation can be established. Truncation errors in the direct minimziation can be tolerated because of the two-step method.Examples of some of the problems solved are given.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690180209
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  • 4
    Electronic Resource
    Electronic Resource
    Multicomponent rates of sorption of SO2 and CO2 in sodium mordenite (1973)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 19 (1973), S. 1055-1059 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690190531
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  • 5
    Electronic Resource
    Electronic Resource
    Transient diffusion in solids with a bipore distribution (1976)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 22 (1976), S. 147-152 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Transient diffusion of n-butane, iso-butane, and 1-butene in synthetic CaX(Na) spherical pellets is studied in a constant volume, well-stirred system. The intracrystalline diffusion coefficients are in the order of 10-14 cm2/s, and the activation energy is about 7.0 Kcal/g-mole. Experimental data agree well with a mathematical model developed to describe transient diffusion.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690220118
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  • 6
    Electronic Resource
    Electronic Resource
    Analysis of liquid chromatography with nonuniform crystallite particles (1990)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 36 (1990), S. 1569-1576 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A mathematical analysis for the pulse responses of a liquid chromatographic column packed with crystal powders having a particle size distribution and a nonlinear adsorption isotherm is presented. The mathematical model is solved numerically by the orthogonal collocation method. Based on the parametric analysis of the model, the effects of a symmetrical and moderately asymmetric PSD on the LC responses are shown to be negligible in comparison with the effects of other parameters, such as isotherm nonlinearity, whose effects are much more profound. The simulated responses are compared with the experimental response data for an LC column packed with silicalite crystals, and a good agreement is found between the theoretical and experimental results. Using the nonlinear LC model, the simultaneous determination of nonlinear adsorption isotherms and intraparticle diffusivities from LC pulse responses is demonstrated for liquids in porous adsorbents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690361013
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  • 7
    Electronic Resource
    Electronic Resource
    Theory of gas diffusion and permeation in inorganic molecular-sieve membranes (1995)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 41 (1995), S. 58-67 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Theoretical models of gas diffusion and permeation in microporous molecularsieve membranes are presented. The effect of the adsorbed diffusant on the total transmembrane flow is insignificant for permanent gases. For highly adsorbable gases the effect of the adsorbed molecules on the total transmembrane flux may be high at low temperatures. The activation energy of diffusion increases when the kinetic diameter of the diffusant increases. The activation energy of gas diffusion compares well with the values calculated based on the Lennard-Jones potential. Maximum possible permeability coefficients calculated for He in the molecular-sieve membranes do not exceed ˜30,000 Barrer at room temperature. The experimentally observed value for He permeability is ˜1,000 Barrer (T=30°C) because of the high tortuosity (τ≍ 25) and low porosity (θ = 0.22) of the membrane porous structure.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690410107
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  • 8
    Electronic Resource
    Electronic Resource
    Diffusion in synthetic faujasite powder and pellets (1974)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 20 (1974), S. 279-284 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Transient sorption rates of allene and methylacetylene in synthetic faujasite (Linde 13X) powder and self-bonded pellets are studied in a constant-volume, constant-pressure system at three temperatures. The intracrystalline diffusion coefficients are found to be in the order of 10-11 cm2/s and the activation energy for diffusion is 4.0 kcal/g-mole.A simple bipore distribution model for transient diffusion in macropores and micropores is presented. It agrees well with the experimental data and quantitatively predicts the macropore diffusion coefficients. The flow in the macropores is found to be in the Knudsen flow regime.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690200211
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  • 9
    Electronic Resource
    Electronic Resource
    Xylene isomerization using zeolites in a gradientless reactor system (1987)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 33 (1987), S. 1233-1240 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A gradientless reactor was used to study the xylene isomerization reaction over a series of cation-exchanged (H+, Li+, Na+, and K+) ultrastable faujasite and on ZSM5 and silicalite to observe the effects of site modification on the reaction. Adsorption, diffusion, and kinetic parameters were determined in the presence of the reaction; the results generally agreed with the available literature data. The gradientless reactor and associated modeling techniques are shown to be effective tools in investigating catalyst behavior. Precise analytical techniques are required to directly measure the diffusion coefficient in the presence of reaction.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690330802
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