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  • 1
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    Chemical and microbiological changes of salted Caspian Kutum (Rutilus frisii kutum) roe (2018)
    Details
    Publication Date: 2021-05-19
    Description: Salted Kutum roe was prepared by soaking in brine to a traditional procedure. Proximate composition (moisture, protein, lipid, pH, ash and salt content), total volatile basic nitrogen (TVB-N), fatty acid profiles and microbiological characteristics of raw and salted roe of Caspian white fish (Rutilus frisii kutum) were determined. The results indicated that protein and moisture contents were significantly higher in raw roe compared to salted one (28.81 and 61.07% to 23.99 and 51.57%, respectively). Significant differences in ash and salt contents were obtained among different treatments. TVB-N in all samples was less than 25mg/100g. The gas chromatography (GC) analysis showed that the major saturated fatty, monounsaturated fatty acids and poly unsaturated fatty acids were 16:0, 18:1n-9, and 22:6n-3, respectively. Aerobic plate count (APC) and Total Coliform Count (TC) in all samples were significantly higher (p〈0.05) in raw roe (5.33 logCFU/g and 210 MPN/g) compared to salted roe (1.23 log CFU/g and 1.2 MPN/g). None of the samples contained Escherichia coli, Salmonella, Clostridium perfringens or yeast.
    Description: Published
    Keywords: Biology ; Physiology ; Clostridium perfringens ; Salmonella ; Escherichia coli ; Caspian Kutum ; Rutilus frisii kutum ; Roe ; Salting ; Microbial population ; Chemical ; Microbiological
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.176-187
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  • 2
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    Microbial and biochemical characteristics of fermented fish sausage from common carp (Cyprinus carpio) mince by application of Pediococcus pentasaceus at different incubation temperatures (2021)
    In:  http://aquaticcommons.org/id/eprint/21949 | 18721 | 2018-01-18 09:32:14 | 21949 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-05
    Description: Fermented sausage is a favorite kind of meat-product that has allocated great proportions of meat consumption in the world. For the first time in Iran in this study the production of Fermented sausage from minced meat of common carp was assessed by means of lactic acid bacteria at different incubating temperatures as 15, 25, and 35˚C. To prepare the fish sausage, common carp mince was grounded and mixed with NaCl (3%), glucose (3%) and lactic acid bacteria at 5 log CFU/g and afterward were incubated for 48 h. During the incubation of fish sausage, microbiological tests, moisture and protein content, and TVB-N were measured. According to the results, the higher temperature of 35˚C stimulated the rapid growth of lactic acid bacteria, resulting in a rapid decline in pH, and consequently suppressed the growth of pseudomonas, Micrococcaceae and Enterobacteriacea.
    Keywords: Biology ; Fisheries ; fish sausage ; common carp ; lactic acid bacteria ; fermentation ; Microbial ; biochemical ; Cyprinus carpio ; application ; Pediococcus pentasaceus ; temperature ; Enterobacteriacea ; Micrococcaceae ; Pseudomonas ; growth ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 35-46
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  • 3
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    Effects of starvation and refeeding on the hematological and serum parameters and body proximate composition of Caspian salmon (Salmo trutta caspius) fingerligs (2021)
    In:  http://aquaticcommons.org/id/eprint/22106 | 18721 | 2018-02-08 04:00:18 | 22106 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-06-26
    Description: This study was conducted to investigate the effects of periods of starvation and refeeding on some hematological, serum parameters and whole body proximate composition in Caspian salmon (Salmo trutta caspius). 240 samples of the Caspian salmon with an average weight of 13.73 ± 0.63 g were kept in 300 liter polyethylene tanks. Samples were fed twice a day with Rainbow trout commercial feed to the point of satiation. This experiment lasted for 10 weeks with control (without starvation) and 3 starvation treatments including 2, 4 and 6 weeks of starvation followed by 4 weeks of refeeding with 3 replicates. During rearing period, the average of water temperature, pH and dissolved oxygen were 14.15±0.27 ° C, 8.44±0.17 and 6.34±0.24 mgl-1, respectively. At the end of starvation period, the results of blood parameters showed that starvation significantly increased red blood cells and hematocrit in treatment with 2 weeks of starvation (0.893±0.58 × 106/mm3) and 6 weeks of starvation (42.33±3.21 %, P〈0.05), respectively. At the end of trial, the highest value of white blood cells belonged to 6 weeks starvation treatment (14.2±0.12 × 103/mm3) while the lowest (10±0.60 × 103/mm3) was observed in the control. Results of the biochemical serum parameters indicated the lowest amount of total protein (1.71±0.88 mg/dl) and glucose (41.35±1.44 mg/dl) were obtained in 6 weeks starvation treatment, right after the starvation period (P〈0.05). At the end of the starvation period, the whole body protein content of control was lower than other treatments (P〈0.05), but after 2 weeks of refeeding, whole body protein content of 2 and 4 weeks starvation treatments didn't show any significant difference compared to control (P〉0.05). At the end of the experiment, the lowest value of lipid belonged to starvation treatments while the highest value was observed in control (P〈0.05). Generally, this can be inferred that Caspian salmon fish can tolerate starvation for 2 weeks without any negative impact on blood and serum biochemical parameters and for 4 weeks on whole body proximate composition.
    Keywords: Aquaculture ; Biology ; Chemistry ; Caspian salmon ; starvation ; refeeding ; proximate composition ; blood ; parameters ; Salmo trutta caspius ; fingerligs ; hematological ; serum ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 161-174
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  • 4
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    Effects of fennel (Foeniculum vulgare) essential oil of diet on some biochemical parameters and salinity stress resistance of kutum (Rutilus kutum) fry (2021)
    In:  http://aquaticcommons.org/id/eprint/22153 | 18721 | 2018-02-15 21:17:35 | 22153 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-06-28
    Description: This study was carried out to investigate the effects of different levels of fennel essential oil (Foeniculum vulgare) on some biochemical parameters and salinity stress resistance of Caspian Kutum (Rutilus kutum) fry. For this purpose, Kutum fry with an average initial weight 0.6±0.002 gr were divided randomly in 5 treatments including 0 (control), 100, 200,400 and 600 mg fennel essential oil/kg diet and fed 3 times a day at a ratio of 7-12% body weight for 60 days. At the end of the experiment, biochemical parameters and salinity stress resistance were assessed. Results of serum biochemical analyze showed that the highest level of total protein and globulin was observed in 600 mg fennel essential oil/kg diet. The highest level of albumin and cholesterol was observed in 100 and 400 mg fennel essential oil/kg diet and the highest level of triglyceride, glucose and cortisol was observed in control (p〈0.05). Results of salinity stress (6, 13 and 20 g/l) showed that 100 and 400 mg fennel essential oil/kg diet treatments showed the lowest response to stress and there were the lowest changes in cortisol and glucose levels before and after stress compare to control. Highest survival was observed in 100 and 400 mg fennel essential oil/kg diet. In conclusion results suggested that Fennel essential oil can improve immune system of fries by promoting biochemical parameters (total protein, albumin, globulin, triglyceride, glucose and cortisol). Also plays important role in increasing stress resistance of Kutum fry and the best operation is related to 100 mg fennel essential oil/kg diet.
    Keywords: Biology ; Fisheries ; Kutum (Rutilus kutum) ; Fennel essential oil ; Biochemical ; parameters ; Survival ; Salinity stress ; Foeniculum vulgare ; fennel ; immune ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 1-16
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  • 5
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    The effect of photoperiod on stomach and intestinal digestive enzymes in rainbow trout (Oncorhynchus mykiss) larvae and juvenile (2021)
    In:  http://aquaticcommons.org/id/eprint/22009 | 18721 | 2018-01-21 12:27:32 | 22009 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-06
    Description: This study investigated the ontogeny of some digestive enzyme and explored the effects of photoperiod on stomach (pepsin) and intestine enzyme activity (alkaline phosphatase and Namino peptidase) in rainbow trout (Oncorhynchus mykiss) larvae juvenile. About 3600 larval rainbow trout in first feeding (18 days after hatching) with an average initial weight 119±0.009 mg were distributed in plastic tanks in four treatments (300 larvae in each replicate). Four light treatments consisting of 14 to 10 h, 10 to 14 h, 4 to 20 h and 24 to 0 h (light: dark), were compared in triplicates for 6 weeks. Finally, a random sampling from larvae was undertaken on days 1, 3, 5, 8, 10, 15, 20, 25, 30, 39 and 44 days after the onset of first feeding. According to the results, the digestive enzymes activity in fish larvae and juveniles had a similar change trend with age at all photoperiods. In addition, at the end of experiment, the specific activity of pepsin and Namino peptidase were the highest in 24h Light treatment, but there was no significant difference in specific activities of alkaline phosphatase (p〉0.05). The results demonstrated that growth and digestive enzymes activity of rainbow trout larvae and juvenile are improved by applying of longer photoperiod in rearing conditions.
    Keywords: Aquaculture ; Biology ; Photoperiod ; Digestive ; enzyme ; Pepsin ; N-amino peptidase ; Alkaline phosphatase ; Rainbow trout ; Oncorhynchus mykiss ; larvae ; juvenile ; hatching ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 103-115
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  • 6
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    Chemical and microbiological changes of salted Caspian kutum (Rutilus frisii kutum) roe (2021)
    In:  http://aquaticcommons.org/id/eprint/22765 | 18721 | 2018-05-21 18:25:04 | 22765 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-09
    Description: Salted kutum roe was prepared by soaking in brine to a traditional procedure. Proximate composition (moisture, protein, lipid, pH, ash and salt content), total volatile basic nitrogen (TVB-N), fatty acid profiles and microbiological characteristics of raw and salted roe of Caspian white fish (Rutilus frisii kutum) were determined. The results indicated that protein and moisture contents were significantly higher in raw roe compared to salted one (28.81 and 61.07% to 23.99 and 51.57%, respectively). Significant differences in ash and salt contents were obtained among different treatments. TVB-N in all samples was less than 25mg/100g. The gas chromatography (GC) analysis showed that the major saturated fatty, monounsaturated fatty acids and poly unsaturated fatty acids were 16:0, 18:1n-9, and 22:6n-3, respectively. Aerobic plate count (APC) and Total Coliform Count (TC) in all samples were significantly higher (p〈0.05) in raw roe (5.33 logCFU/g and 210 MPN/g) compared to salted roe (1.23 log CFU/g and 1.2 MPN/g). None of the samples contained Escherichia coli, Salmonella, Clostridium perfringens or yeast.
    Keywords: Biology ; Chemistry ; Fisheries ; Rutilus frisii kutum ; Roe ; Salting ; Microbial population ; Biology ; Physiology ; Caspian Sea ; Iran ; Chemical ; microbiological
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 176-187
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  • 7
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    Seasonal variation of chemical composition and fatty acid profile of ovary in wild common carp (Cyprinus carpio) of southeastern Caspian Sea (2021)
    In:  http://aquaticcommons.org/id/eprint/23776 | 18721 | 2018-07-27 13:25:54 | 23776 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: Chemical composition and fatty acid profile of common carp's gonad (ovary) were assessed. Protein, lipid, fatty acid profile and moisture content were determined during 4 seasons’ summer, autumn, winter in 2007 and spring in 2008. For each season 10 samples were examined. Average of gonadosomatic index in wild common carp was 7.53 ±5.02. Proximate composition of ovary during the study period showed the lipid at 8.06 ±2.20; protein at 23.26 ±4.85; and moisture at 67.12 ±3.85. The results showed lipid content of wild fish ovary increased from summer to spring (summer 6.875 ±0.53; autumn 7.07 ±2.12; winter 7.96 ±1.22; spring 9.44 ±3.62), protein content also increased from summer to spring (summer 12.920.09; autumn 23 ±1.32; winter 25.16 ±0.63; spring 27.11 ±0.63), moisture content decreased in this period (summer 75.235 ±1.75; autumn 68.25 ±2.28; winter 65.685 ±0.40; spring 63.43 ±0.11). Significant differences (P0.05). Saturated and polyunsaturated fatty acids increased from summer to spring (spawning season). The major fatty acids identified in common carp ovary were Oleic (C18:1), palmitic (C16:0), Docosahexanoic acid (C22:6 DHA), Palmitoleic (C16:1), Arachidonic, AA (C20:4), Stearic (C18:0), Eicosapentaenoic acid (C20:5 EPA) and Linoleic (C18:2) in all seasons. Lipid, protein and omega 3 PUFA increased during gonad maturation. It seems that these resources of energy are necessary for embryogenesis.
    Keywords: Biology ; Cyprinus carpio ; Ovary ; Fatty Acid ; Iran ; Caspian Sea ; Caspian Sea Eurasia
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 151-160
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  • 8
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    Insulin-like growth factor I gene polymorphism associated with growth traits in beluga (Huso huso) fish (2021)
    In:  http://aquaticcommons.org/id/eprint/23158 | 18721 | 2018-06-18 22:41:32 | 23158 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-13
    Description: The aim of the present study was to detect polymorphism in Insulin like growth factor-I (IGF-I) gene of beluga (Huso huso) fish using PCR-SSCP technique and also investigation of its association with growth traits (condition factor, body length and weight). A total of 150 specimens of beluga were randomly selected and DNA was isolated from caudal fin using modified salting out method. Then two fragments of 171 and 362 bp from 5'-UTR and 3'-UTR regions of IGF-I gene were amplified, respectively. Genotyping of individuals by SSCP technique showed five banding patterns of A, B, C, D and E for 5'-UTR region with the frequencies of 29.2, 0.76, 16.92, 51.53 and 10% respectively in one year-old and three banding patterns of A, C and D with the frequency of 45, 10 and 45% for two year-old fish. Also three banding patterns (A, B and C) were seen for 3'-UTR region with the frequency of 62.3, 27.69 and 10.76% in one-year-old and 20, 60 and 20% in two year-old fish. The A banding pattern in 3'-UTR and D banding pattern in 5'-UTR sites were the most frequent pattern in the studied beluga population. The association analysis using SAS statistical software indicated no significant association between observed banding patterns and growth traits (body length, weight, and condition factor) in beluga. Considering the important role of IGF-I as a probable candidate gene affecting growth related traits, these marker sites should be studied more in larger sample sizes and also in other regions of the gene.
    Keywords: Biology ; Polymorphism ; Beluga ; 3'-UTR ; 5'-UTR ; PCR-SSCP ; Growth traits ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 1257-1266
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