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  • Engineering General  (16)
  • Biochemistry and Biotechnology  (10)
  • Wiley-Blackwell  (26)
  • 1
    Electronic Resource
    Electronic Resource
    Design, construction, and operation of some all-glass pilot-plant fermetors (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 20 (1978), S. 1345-1375 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The design, Construction, and operation of a 400 liter all-glass fermentor, made from industrial glass Components, is described in detail. Outline details are also given for 100 and liter vessels of similar construction. The performance of the 400 liter fermentor with a variety of organisms is discussed. Harvesting performance. Using a disk-stak centrifuge, is also described.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260200904
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  • 2
    Electronic Resource
    Electronic Resource
    Two-step immobilized enzyme conversion of cephalosporin C to 7-aminocephalosporanic acid (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 46 (1995), S. 510-513 
    Details
    ISSN: 0006-3592
    Keywords: cephalosporin C ; production of 7ACA ; immobilized D-amino acid oxidase ; immobilized GL-acylase ; industrial enzyme process ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The first large-scale production of 7-aminocephalosporanic acid (7ACA) from cephalosporin C (CPC) using a wholly enzymatic synthesis method is reported here. We produced 7ACA from CPC in as high a molar yield as 85% using the immobilized enzymes D-amino acid oxidase (D-AOD) and glutaryl-7-ACA acylase (GL-acylase). In the first reactor, CPC is converted to keto-adipyl-7-aminocephalosporanic acid (keto-7ACA) using an immobilized D-AOD isolated from a yeast, Trigonopsis variabilis. The keto-7ACA is then spontaneously converted to glutaryl-7-aminocephalosporanic acid (GL-7ACA) via a chemical reaction with hydrogen peroxide. The hydrogen peroxide is also a product of the D-AOD reaction. Near quantitative conversion of the keto-7ACA to GL-7ACA was observed. The second reactor converts GL-7ACA to 7ACA using an immobilized GL-acylase, which was isolated from a reconbinant Escherichia coli. The final 7ACA crystalline product is a high quality product. The reactions are conducted under very mild aqueous conditions: pH 8.0 and 20° to 25°C. The production of desacetyl side products is minimal. This process is currently being implemented on an industrial scale to produce 7ACA. © 1995 John Wiley & Sons, Inc.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260460603
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  • 3
    Electronic Resource
    Electronic Resource
    The human myocardial two-dimensional gel protein database: Update 1994 (1994)
    Weinheim : Wiley-Blackwell
    Electrophoresis 15 (1994), S. 1459-1465 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: An updated human heart protein two-dimensional electrophoresis (2-DE) database is presented. The database, which contains some 1388 protein spots characterised in terms of Mr and pI, has been analysed further by Western immunoblotting and protein sequencing. From a total of 103 protein spots analysed, 49 have been identified by immunoblotting and 32 have been identified by protein sequencing. A further six proteins have tentatively been assigned by comparison with the human heart 2-DE protein database of Jungblut et al. (Electrophoresis) 1994, 15, 685-607). This database is being used in studies of alterations in protein expression in the diseased and transplanted human heart.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501501209
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  • 4
    Electronic Resource
    Electronic Resource
    Evaluation of membranes used for electroblotting of proteins for direct automated microsequencing (1991)
    Weinheim : Wiley-Blackwell
    Electrophoresis 12 (1991), S. 342-348 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Protein purification and characterisation have been age-old problems for the biochemist. A new era has arisen with the advent of one- and two-dimensional gel electrophoresis and high sensitivity automated protein microsequencing. These two tools along with electroblotting have made it possible to separate and analyse complex protein mixtures. We studied six different membranes compatible with Edman degradation chemistry to determine their efficiencies at binding proteins electroblotted from one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Their overall blotting-sequencing properties were also evaluated. We found that the polyvinylidene difluoride-based membranes out-performed the glass-based and polypropylene-based membranes under our selec ted experimental conditions. The problems associated with electroblotting and microsequencing are discussed.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150120505
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  • 5
    Electronic Resource
    Electronic Resource
    On a finite element CFD algorithm for compressible, viscous and turbulent aerodynamic flowsBased as an invited lecture. (1987)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 7 (1987), S. 1235-1259 
    Details
    ISSN: 0271-2091
    Keywords: Fluid Mechanics ; CFD ; Finite Element ; Accuracy/Convergence ; Stability ; PNS/TLNS ; Turbulent ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper develops and analyses individual construction aspects of an efficient and accurate finite element algorithm for prediction of viscous and turbulent flow fields of impact in aerodynamics. The theoretical construction employs a Taylor weak statement (TWS) for coincident embedding of stability mechanisms within a classic Galerkin finite element formulation of semi-discrete approximation error orthogonalization. A wide variety of the stabilizing mechanisms of independently derived CFD algorithms are contained within the TWS theory. An implicit construction that meets the requirement of efficient convergence to steady state is developed. The theoretical asymptotic error estimates of the TWS finite element algorithm for supersonic and viscous boundary layer flows are verified. Application to a three-dimensional turbulent flow is cited.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650071107
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  • 6
    Electronic Resource
    Electronic Resource
    A globally well-posed finite element algorithm for aerodynamics applications (1991)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 12 (1991), S. 407-441 
    Details
    ISSN: 0271-2091
    Keywords: Compressible Navier-Stokes equations ; Taylor weak statement ; Curvilinear co-ordinate dissipation ; Lyapunov stability theory ; Well-posed boundary conditions ; Finite element semi-discretization ; Implicit Rosenbrock-Runge-Kutta scheme ; Tensor matrix product factorization ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A finite element CFD algorithm is developed for Euler and Navier-Stokes aerodynamic applications. For the linear basis, the resultant approximation is at least second-order-accurate in time and space for synergistic use of three procedures: (1) a Taylor weak statement, which provides for derivation of companion conservation law systems with embedded dispersion-error control mechanisms; (2) a stiffly stable second-order-accurate implicit Rosenbrock-Runge-Kutta temporal algorithm; and (3) a matrix tensor product factorization that permits efficient numerical linear algebra handling of the terminal large-matrix statement. Thorough analyses are presented regarding well-posed boundary conditions for inviscid and viscous flow specifications. Numerical solutions are generated and compared for critical evaluation of quasi-one- and two-dimensional Euler and Navier-Stokes benchmark test problems. Of critical importance, essentially non-oscillatory solutions are uniformly attained for a range of supercritical flow situations with shocks.
    Additional Material: 24 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650120502
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  • 7
    Electronic Resource
    Electronic Resource
    Determination of the critical slip surface in slope stability computations (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 4 (1980), S. 333-359 
    Details
    ISSN: 0363-9061
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: The analysis of the stability of slopes using limiting equilibrium considerations necessitates the determination of the critical slip surface which yields the minimal factor of safety. The numerous methods currently available for slope stability analysis provide a procedure for assigning a factor of safety to a given slip surface, but do not consider the problem of identifying the critical conditions.This paper presents an effective minimization procedure based on dynamic programming by which the minimal factor of safety, and the corresponding surface, are determined simultaneously. This procedure SSDP (Slope Stability by Dynamic Programming), couples the minimization scheme with Spencer's method of slope stability analysis. It may be applied to slopes of any geometry, layering, pore pressure and external load distributions. No arbitrary restrictions are placed on the shape of the slip surfaces, and the analysis satisfies all equilibrium equations.Application of the procedure to slope stability problems reported in the literature shows that for a given slip surface the procedure yields factors of safety which are almost identical to those reported, but in every case a more critical slip surface, with a lower factor of safety, may be found.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nag.1610040405
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  • 8
    Electronic Resource
    Electronic Resource
    Consequences of deviatoric normality in plasticity with isotropic strain hardening (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 6 (1982), S. 383-390 
    Details
    ISSN: 0363-9061
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: For many soils experimental evidence suggests that deviation from normality between the plastic strain increments and the yield function is exhibited mainly in the volumetric behaviour, whereas the deviatoric behaviour exhibits normality. An analysis of the consequence of this observation is presented, and it leads to an expression for the plastic potential function as composed of two functions, a yield function and another dependent only on the hydrostatic pressure; the latter is considered to constitute the concept of correction functions presented recently by Desai and Siriwardane13.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nag.1610060308
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  • 9
    Electronic Resource
    Electronic Resource
    Slope stability analysis for undrained loading conditions (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 17 (1993), S. 15-43 
    Details
    ISSN: 0363-9061
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: The current state of art for limit equilibrium analysis of slope stability problems lacks a satisfactory procedure for stability evaluation under general, rapid (undrained) loading conditions. Some procedures are available for the analysis of rapid drawdown, but these suffer from several shortcomings and, furthermore, are not applicable to other types of rapid loading. An approach is presented which overcomes these limitations. The approach integrates four components-establishment of soil behaviour on the basis of laboratory testing, estimation of steady-state conditions in the slope using a boundary value analysis, estimation of distribution of undrained strength in the slope using undrained stress paths, and identification of the critical slip surface followed by calculation of its factor of safety. The approach is illustrated through its application to the stability analysis of an earth dam under rapid drawdown and earthquake conditions.
    Additional Material: 17 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nag.1610170103
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  • 10
    Electronic Resource
    Electronic Resource
    On Taylor weak statement finite element methods for computational fluid dynamics (1995)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 21 (1995), S. 273-294 
    Details
    ISSN: 0271-2091
    Keywords: finite element ; finite volume ; Taylor weak statement ; Taylor-Galerkin method ; phase velocity ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A Taylor series augmentation of a weak statement (a ‘Taylor weak statement’ or ‘Taylor-Galerkin’ method) is used to systematically reduce the dispersion error in a finite element approximation of the one-dimensional transient advection equation. A frequency analysis is applied to determine the phase velocity of semi-implicit linear, quadratic and cubic basis one-dimensional finite element methods and of several comparative finite difference/finite volume algorithms. The finite element methods analysed include both Galerkin and Taylor weak statements. The frequency analysis is used to obtain an improved linear basis Taylor weak statement finite element algorithm. Solutions are reported for verification problems in one and two dimensions and are compared with finite volume solutions. The improved finite element algorithms have sufficient phase accuracy to achieve highly accurate linear transient solutions with little or no artificial diffusion.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650210402
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