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  • 1
    Electronic Resource
    Electronic Resource
    Large-scale cultivation of Solanum chrysotrichum cells: Production of the antifungal saponin SC-1 in 10-l airlift bioreactors (1997)
    Springer
    Plant cell reports 16 (1997), S. 653-656 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsSolanum chrysotrichum ; Cell suspension culture ; Spirostanol saponins ; Antimycotic ; Large-scale culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of two different cell lines: ccvx (cotyledon derived) and ccvz (hypocotyl derived) of Solanum chrysotrichum were cultivated in 10-l airlift bioreactors for the production of the human antimycotic compound SC-1. When using 3 g l–1 dry weight inoculum in a batch culture, higher levels of biomass were achieved with the ccvx cell line (14.6 g l–1) than with ccvz (7.7 g l–1), resulting in 23 and 12 mg g–1 of SC-1 after 17 days in culture for ccvx and ccvz, respectively. The maximum productivity of SC-1 in bioreactors was 0.025 g l–1 day–1 after 9 days in culture. When using a draw-fill mode, the productivity increased by 60% to a value of 0.041 g l–1 day–1, 4 days after 50% of the cell suspension was removed and replaced with fresh medium. This latter bioreactor system is a feasible alternative for the production of the antimycotic metabolite of S. chrysotrichum on a large scale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050296
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  • 2
    Electronic Resource
    Electronic Resource
    Large-scale cultivation ofSolanum chrysotrichum cells: Production of the antifungal saponin SC-1 in 10-I airlift bioreactors (1997)
    Springer
    Plant cell reports 16 (1997), S. 653-656 
    Details
    ISSN: 1432-203X
    Keywords: Solanum chrysotrichum ; Cell suspension culture ; Spirostanol saponins ; Antimycotic ; Large-scale culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of two different cell lines:ccvx (cotyledon derived) andccvz (hypocotyl derived) ofSolanum chrysotrichum were cultivated in 10-1 airlift bioreactors for the production of the human antimycotic compound SC-1. When using 3 g l-1 dry weight inoculum in a batch culture, higher levels of biomass were achieved with theccvx cell line (14.6 g l-1) than withccvz (7.7 g l-1), resulting in 23 and 12 mg g-1 of SC-1 after 17 days in culture forccvx andccvz, respectively. The maximum productivity of SC-1 in bioreactors was 0.025 g l-1 day-1 after 9 days in culture. When using a draw-fill mode, the productivity increased by 60% to a value of 0.041 g l-1 day, 4 days after 50% of the cell suspension was removed and replaced with fresh medium. This latter bioreactor system is a feasible alternative for the production of the antimycotic metabolite ofS. chrysotrichum on a large scale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275509
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  • 3
    Electronic Resource
    Electronic Resource
    A minimal multiconfigurational technique (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 7 (1986), S. 201-207 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: A direct minimization method previously presented by the authors is applied here to biconfigurational wave functions. A very moderate increasing in the time by iteration with respect to the one-determinant calculation and good convergence properties have been found. So qualitatively correct studies on singlet systems with strong biradical character can be performed with a cost similar to that required by Hartree-Fock calculations.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540070213
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  • 4
    Electronic Resource
    Electronic Resource
    Restricted Hartree-Fock approximation. I. Techniques for the energy minimization (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 4 (1983), S. 33-40 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: We present a new procedure for direct minimization of the RHF energy, which presents advantages over SCF methods with respect to convergence rate and computational cost. In this procedure we combine several techniques with the aim of obtaining best directions and step lengths for the iterative search for a minimum of the energy. In this article we develop the theory. Therefore, we analyze the variational function; we present a short description of the minimization techniques and we discuss in detail the way in which they are to be used. The computational aspects of the procedure will be treated in the following article.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540040106
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  • 5
    Electronic Resource
    Electronic Resource
    Approximate STO functions for the first-row transition metal atoms (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 12 (1991), S. 1172-1181 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: Slater type orbital (STO) basis sets for the atoms Sc-Zn have been derived using a technique based on the distance between subspaces. The accuracy for several properties of these basis sets has been tested. Basis sets studied are of both single- and double-zeta sizes, although this technique can be generalized for any size. Uniform quality criteria through the series of atoms Sc-Zn are difficulty to establish due to the varying number of d electrons. A comparative study at the atomic level of the quality of STO basis sets (both the two new basis sets and Clementi's basis sets) for the first-row transition elements has been carried out. Results show that the new basis sets provide better simulation for several properties. Molecular calculations on compounds with these atoms using a Gaussian expansion fitted according to the new values of optimized STOs are also included. The results obtained are similar to those reported when STO-3G basis set is used.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540121003
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