Publication Date:
2005-02-01
Description:
DNA translocases are molecular motors that move rapidly along DNA using adenosine triphosphate as the source of energy. We directly observed the movement of purified FtsK, an Escherichia coli translocase, on single DNA molecules. The protein moves at 5 kilobases per second and against forces up to 60 piconewtons, and locally reverses direction without dissociation. On three natural substrates, independent of its initial binding position, FtsK efficiently translocates over long distances to the terminal region of the E. coli chromosome, as it does in vivo. Our results imply that FtsK is a bidirectional motor that changes direction in response to short, asymmetric directing DNA sequences.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pease, Paul J -- Levy, Oren -- Cost, Gregory J -- Gore, Jeff -- Ptacin, Jerod L -- Sherratt, David -- Bustamante, Carlos -- Cozzarelli, Nicholas R -- GM07232-27/GM/NIGMS NIH HHS/ -- GM08295-15/GM/NIGMS NIH HHS/ -- GM31657/GM/NIGMS NIH HHS/ -- GM32543/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2005 Jan 28;307(5709):586-90.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3204, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15681387" target="_blank"〉PubMed〈/a〉
Keywords:
Algorithms
;
Bacteriophage lambda
;
Base Sequence
;
Chromosomes, Bacterial
;
DNA, Bacterial/chemistry/*metabolism
;
DNA, Superhelical/chemistry/metabolism
;
DNA, Viral/chemistry/*metabolism
;
Escherichia coli/*metabolism
;
Escherichia coli Proteins/isolation & purification/*metabolism
;
Kinetics
;
Membrane Proteins/isolation & purification/*metabolism
;
Models, Biological
;
Molecular Motor Proteins/isolation & purification/*metabolism
;
Nucleic Acid Conformation
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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