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  • Articles  (11)
  • Aquaculture  (11)
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  • Articles  (11)
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  • 1
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25191 | 18721 | 2018-09-03 17:30:30 | 25191 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The present study was conducted to survey the artificial spawning of European catfish (Silurus glanis L.) by synthetic hormones include Carp pituitary extract (CPE), Ovo fact [D-Ala6 des Gly10] GnRHa Ethyl amid in combination with domperidone diluted in propylene glycol, Human gonadotropin chryonic hormone (HCG) and LHRHa (des-Gly10, [D-Ala6] LH-RH Ethyl amide). In this research work 44 male and 72 female brood stokes that transferred to the Shaheed Dr Ansari hatchery and Sefid rod research station were studied on 2 years and injected with those stimulated maturation. Total length, total weight, gonad weight were measured to the nearest 0.1 cm, 1g and 0.01g respectively. The experiments were conducted using different doses of hormones on 4 treatments. In treatment CPE that brood stocks injected with 2.5, 3, 4 and 5 mg/kg/BW, the most responded (83.33%) was with 5mg/kg/Bw of CPE. The mean fertilization in this treatment was 92.5 4.51 and after that there are groups which were stimulated by 4 mg/kg/BW of CPE .In treatment ovafact that brood fish were injected by 10, 20, 30, 40 μg/kg/BW of this stimulation hormone showed 100 percent of fish ovulated in the group treated with 40 μg/kg/BW of ovofact. The mean fertilization rate was 86.08 6.27 in this group. 33.3 % of brood fish ovulated in the group that injected with 40 μg/kg/BW of LHRHa stimulation, but none of brood stock reached to spawning with injection of 30 μg/kg/BW of LHRHa. The brood stocks were injected by 800, 850, 900, 950 IU/kg/BW of HCG, only 33.3 percent of fish in groups that received 900 & 950 IU/kg/BW of this Stimulation hormone reached to spawning stages. According to our results and statistical analysis between trial treatments in percent of Embryo viability showed significant difference (P〈0.05) between HCG treatment with Ovofact and CPE groups. This results indicates that embryo viability was better in CPE and Ovofact rate to LHRHa and HCG treatments. In the other cases include the working fecundity, fertilization percent, latency period of maturation, latency period of incubation eggs and ratio of weigh stripped egg to body weight did not show any significant difference (P〈0.05) in experimental treatments for stripping the male brood stock of European catfish were injected by 3,4,5 mg/kg/BW of CPE, but not one of male brood fish didn’t reaches to stripping stage , so for eggs fertilization were used of milt from macerated Testes of dead males. So in anther experiment the male brood fish were injected by 5 mg/kg/BW CPE repeatedly at once every 5 days for Period 20 day’s period and milt were collected from of all of the males.
    Keywords: Aquaculture ; Iran ; Silurus glanis ; Artificial propagation ; CPE ; Ova fact ; HCG ; LHRHa ; Spawning ; Hormones ; Carp ; Maturation ; Brood stocks ; Fecundity ; Fertilization ; European Catfish ; Fingerling
    Repository Name: AquaDocs
    Type: monograph
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  • 2
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25612 | 18721 | 2018-10-07 16:15:05 | 25612 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Kutum fish (Rutilus kutum) is an anadromous cyprinid species that spawns in adjusted rivers to the Caspian Sea. Many million fingerlings were artificially produced and annually released in these rivers specifically in Sefidrud River by Iranian Fisheries Organization. In this study were estimated the staying period of Kutum fingerlings in Sefidrud River and its diet and growth. A small piece of the caudal fin was cut as a tagging method. About 50000 and 5200 individuals were marked during July and September 2013, respectively. The marked fish had an average total length 53.2±5.6 mm and body weight 1.11±0.26 g and were released in river around two kilometers of the estuary. The sampling of fishes had been earlier started from Feb. 2012 and continued until Feb. 2013 at five stations. Four stations were along river from three Kilometer of estuary to estuary and one station was in right side of estuary in the sea coast. The results showed that marked samples after releasing time were distributed in all studied area from 3 kilometers of estuary to estuary but they migrate mainly toward Caspian Sea in a short time. However the marked samples were observed in estuary and the sea coast after 6 and 24 hours of their releasing time, respectively, Kutum fingerlings remained in Sefidrud river ecosystem in a longer time, at least until end of our sampling period i.e. 105 days. The coefficient of vacuity index of the natural feeding study was 0.0, 49.0 and 30.6 % for phytoplankton, zooplankton and benthos, respectively. The average of GastroSomatic index, intensity of fullness and condition factor were calculated 6.30±3.25, 174.6±153.2 and 0.92±0.09, respectively. A temporal and spatial variation on main food items were observed in gut content of fish fingerlings from different locations and seasons. Gut contents were generally included 59 genera of phytoplankton, 15 groups of zooplankton and 10 groups of benthos. Nitzschia, Navicula and Synedra were dominated among phytoplankton genera with 45.83, 18.02 and 15.99 %, respectively. Among zooplankton groups; the Rotaria, Moeina and Difflugia were dominated with 22.30, 20.86 and 15.83%, respectively. Chironomidae and Gammaridae were the main food items among fed benthic animals with 83.67 and 11.15%, respectively. Furthermore 48 phytoplankton genera, 32 zooplankton genera and 6 benthic families were identified in ecosystem of Sefidrud river. Bacillariophyta phylum (with 23 genus), Rotatoria (with17 genus) and Chironomidae family were dominated in each mentioned groups, respectively. It is concluded that the Kutum fingerlings stay in Sefidrud River for a long time, although most of them migrate to Caspian Sea during first month after releasing. However dietary indices were normal in studied samples, the condition factor index seems to be low.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Rutilus kutum ; Fingerlings ; Kutum ; Fish ; Rotaria ; Moeina ; Difflugia ; Phytoplankton
    Repository Name: AquaDocs
    Type: monograph
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  • 3
    Publication Date: 2021-07-16
    Description: Rutilus frisii kutum is one of the important fish of Caspian Sea which has significant economical role in the region .The objective of this project is a comparative study on traditional culture of fishes versus slurry. This survey were conducted on nine pound of 1.7 hec triplicate with their treatment with stocking densities of 1.7 million fish larva at Dr yousefpoor center (Affiliated of Shahid Beheshti culture and propagation) at Siakal village , 32 km far from Rasht city in North of Iran, the first treatment considered as control which practice traditionally (using concentrated food plus grinded kilka fish) .In the second treatment for 13 days the pound supplied only by slurry then followed by concentrated fish food only .The tired treatment started with slurry for 13 days and followed by slurry plus concentrated food. The physical and chemical parameters of water, plankton, biometry of fish, growth indices such as daily weight growth (DWG), daily length growth (DLG) as well as specific ratio (SGR) were monitored weekly. The result indicate that net fish production was 1.7 to 2 times higher in slurry than traditional treatment also the survival rate were 1.7 times higher in slurry treatment . The treatment of slurry follow by addition of concentrated food plus slurry showed higher yield camper to others. Slurry with several active substances is more effective and can promote the growth of zooplanktons which is the food of larval stage of rutilus frisi kutum. In second phase of this project the effect of slurry was surveyed on Chinese carp. This experiment was conducted in triplicate with two treatment (one treatment is control which use cow manure plus chemical fertilizer and second treatment was conducted with slurry) and stocked with 2375 N/hec (Silver carp 60%, Big head 15%, common carp 17% and grass carp 8%) at June. In this survey common carp and grass carp were feed with concentrate food and fresh grasses respectively. Chemical factors were measured once for each two week and density of plankton was determined when it was necessarily. Nutritional content of phosphorous, nitrogen, calcium, potassium and magnesium after anaerobic fermentation as well as protein and lipid content with 1.4and 1.9 times respectively were higher in slurry treatment than control. The results showed that survival rate and yield were higher in slurry than control. The increasing percent of yield were 13.5, 2.6, 18.4 and 85.3 in Silver carp, Big head, common carp and grass carp respectively. The survival rate of grass carp was twice higher in slurry treatment than control. Zooplankton abundant in slurry pounds was more but blue- green algae density was less than control. Protein and lipid analysis reviled no significant difference between control and slurry. In general the result indicate that the slurry with higher nutritional content is more effective on the survival and growth rate of fishes and also is more efficient in proliferation of plankton in particular zooplankton.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Slurry ; Rutilus frisii kutum ; Cow fertilize ; Larviculture ; Carp ; Culture ; Plankton ; SGR ; Zooplanktons ; Silver Carp ; Big head carp ; Common carp ; Grass carp ; Phosphorous ; Nitrogen ; Survival rate ; Protein ; Lipid
    Repository Name: AquaDocs
    Type: monograph
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  • 4
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25530 | 18721 | 2018-10-05 15:55:00 | 25530 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This project was conducted to goal of optimum dosage determination of ovaprim injection to artificial spawning efficiency of Esox lucius. The research implemented by 4 treatments with 3 replicates for each ones. 3 female and 6 male brooders injected in each replicate. The animals in 1, 2 and 3 treatments injected by 10, 20 and 30 µg/kg BW, respectively, and 4th treatment as a control injected with 4 mg/kg BW pituitary gland extract. Average weight of brooders were 1361±521, 1376±954, 1009±160 and 1100 ±422 g in 1, 2, 3 and 4 treatments in females and 689±145, 734±197, 547±118 and 794±238 g in males, respectively. In addition, positive response percent to hormone injection were measured 77.8 ±19.24 , 88.9 ± 19.24 , 55.5 ±50.91 and 55.5 ± 19.24 % in 1, 2, 3 and 4 treatments in female and 94.4 ± 9.58, 88.9 ±19.26 , 83.3±28.86 and 88.9 ± 19.26 % in male brooders, respectively, but there was no significant different between all of treatments (p〈0.05). Incubation period from fertilization till hatching step in 7 to 15 ˚C was 5 to 10 days with average of 7±1.5 days. Fertilization content was in 1 to 4 treatments measured 87.1±10, 88.04±7.7, 83.9±5.2 and 72.4±19.7 %, respectively and also the treatments didn’t show any different significantly together (p〈0.05). Average percentage of eyed eggs 66.6±15.9 in treat 1, 61.2±22.3 in treat 2, 58.3±10.7 in treat 3 and 56.1±15.04 in treat 4, without any significant different between of them (p〈0.05). Hatching of eggs mean were measured 27.41±19.8 in treat 1, 39.53±26.9 in treat 2, 95.18±5.6 in treat 3 and 26.78±12.4 in treat 4, and significant different observed between of them too (p〈0.05).In the other hand, mean percent of larvae with active feeding in these treatments were measured 18.77±14.6, 20.1±8.51, 55.6±11.6 and 14.51±7.72 as the treatments had significant different (p〈0.05). Also, the best temperature and dosage injection of ovaprim hormone was 9 to 12.5 ˚C and 20µg/kg BW, respectively. The end of trial, from 103740 larvae introduced to earthen pond obtained 8000 fingerlings with weight of 2.68±0.6 g and length of 6.96±0.51 cm.
    Keywords: Aquaculture ; Iran ; Esox Lucius ; Reproduction ; Spawning ; Ovaprim Injection ; Fecundations ; Hatching ; Brooders ; Females ; Males ; Fertilization ; Hormone ; Larvae ; Earthen pond ; Fingerlings
    Repository Name: AquaDocs
    Type: monograph
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  • 5
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25589 | 18721 | 2018-10-07 10:35:13 | 25589 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The study was done in Nutrition and Live Food Station was located in Bandar Anzali Ghaziyan. Juveniles weighted average 0.5, 1, 2.5, 5, 10, 15 and 20 g were randomly selected in three water conditions with a salinity of 11 ppt (Caspian sea water), water 7 ppt and freshwater (with three replicates per group) were included. At intervals of 0, 3, 6, 12, 24, 72, 168, 240 hours, blood samples were heparinized capillary tubes by caudal juveniles and ion concentrations Mg, Ca,Cl using the spectrophotometer and sodium and potassium ions with Flaym photometry (flame photometric), the osmotic pressure of blood plasma by osmometer and cortisol levels were measured by RIA method. To study the microstructure of gill and kidney tissue for each treatment , tissue samples by classical histological methods and stained with hematoxylin - eosin slides were prepared. The frequency and location of the enzyme Na^+, K^+ - ATPase and chloride cells with immunohistochemical localization was performed. Studies micrometric gill chloride cells and renal glomerular networks by software Image tool (version 2.0) was performed. Measurement of enzyme Na^+, K^+-ATPase, by Zaugg (1982) method was conducted. Data analyzed by one-way ANOVA (Oneway ANOVA) with Tukey's test was performed. Overall, the results of measuring ions and osmotic pressure on the tenth day of treatment, the osmotic potential juveniles 2.5, 5, 10, 20 gr in Caspian sea water and all groups except the 0.5 in water of 7 ppt confirmed. But in case of unfavorable conditions for the release in estuaries river and river, fish with weight 1 to 3 release directly to beach (where the salinity is 7 grams per liter) and fishes with weight from 10 to 20 gr to sea. Although suitable river conditions necessary condition for release of juveniles in riverine areas to adaptation juveniles occur gradually.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Rutilus frisii kutum ; Osmolarity ; Suitable size ; Release
    Repository Name: AquaDocs
    Type: monograph
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  • 6
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    In:  http://aquaticcommons.org/id/eprint/22022 | 18721 | 2018-01-25 06:53:02 | 22022 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-06
    Description: This project was conducted to explore the effect of optimum dosage of Ovaprim injection on the artificial spawning of Esox lucius. The research implemented by 4 treatments with 3 replicates for each one. Three female and six male brooders were injected in each replicate. The animals in 1, 2 and 3 treatments were injected by 10, 20 and 30 µg/kg BW, respectively, and 4 the treatment was considered as the control being injected with 4 mg/kg BW pituitary gland extract. Average (±SE) weights were 1361±521, 1376±954, 1009±160 and 1100 ±422 g in 1, 2, 3 and 4 treatments in females, respectively. In addition, positive response percent to hormone injection were measured as 77.8 ±19.24, 88.9 ± 19.24, 55.5 ±50.91 and 55.5 ± 19.24 % in 1, 2, 3 and 4 treatments in female and 94.4 ± 9.58, 88.9 ±19.26, 83.3±28.86 and 88.9 ± 19.26 % in male brooders, respectively. However, there was no significant different between all treatments. Fertilization content (±SE) in one to four treatments measured as 87.1±10, 88.04±7.7, 83.9±5.2 and 72.4±19.7 %, respectively. No significant differences were found among pairwise treatments. Average (±SE) percentage of eyed eggs were 66.6±15.9 for treatment one, 61.2±22.3 in treatment two, 58.3±10.7 in treatment three, and 56.1±15.04 in treatment four, with no significant pairwise differences. The average (±SE) hatching eggs were measured as 27.41±19.8 in treatment one, 39.53±26.9 in treatment two, 95.18±5.6 in treatment three and 26.78±12.4 in treatment four, with no significant pairwise differences. Also, the best dosage injection of ovaprim hormone was 10 and 20 µg/kgBW.
    Keywords: Aquaculture ; Biology ; Chemistry ; Esox Lucius ; Reproduction ; Spawning ; Ovaprim hormon ; Fecundations ; Eyed eggs ; female ; hormone ; hatching ; Iran
    Repository Name: AquaDocs
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  • 7
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25354 | 18721 | 2018-09-13 13:02:58 | 25354 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: 500000 juvenile Indian majour carps (Labeo rohita,Cirhinus merigala, Catla catla) imported (2004) and transferred to the earthen ponds in Shahid Ansari Carp Breeding Center , In order to assess the viability of rearing under the climatic condition of north part of Iran and also achieve some growth parameters from the larval stage to market size.The fry with 300 mg weight, were released in to 8 ponds.specimens were separated to tree experimental categories: 1= (Labeo rohita) , 2= (Cirhinus merigala) , 3= (Catla catla). fishes were fed with artificial food and also with the natural products of the ponds which were enriched with organic fertilizers, after being equally stocked in the ponds .During the experiment, oxygen level ,PH, turbidity, and temperature were measured . Monthly fry samples were taken, for weight and length biometrical analysis and also determination of the weight gain percentage, average daily growth rate, specific growth rate. Results have indicated adaptations and considerable growth in this species, with an increase from the initial weight of 300 mg to an average of 13.5±1.6 mg in 50 days period until the end of November for fingerlings. Also the average weight of growing phase (second year of production) was 647g. For Labeo rohita ,420 g. for Cirhinus merigala and 734g. For Catla catla. The special growth rate(SGR)in Roho was 4.51 %.and 3.9 % for merigal and6.2 % for Catla while for Chinees carps SGR was 4.9, 6.7, 7.6 and 5.8 for silver carp,grass carp, common carp and big head respectively. Results revealed that the Indian majour carps compatibility with the condition in Gilan province was succesfull and the combination of species and optimization of their compatibility were remind for further studies.
    Keywords: Aquaculture ; Iran ; Indian carp ; Growth ; Labeo Rohita ; Cirhinus merigala ; Catla catla ; Juvenile ; Breeding ; Rearing ; Growth ; Larval stage ; Fry ; Artificial food ; Oxygen level ; pH ; Turbidity ; Temperature ; Species
    Repository Name: AquaDocs
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  • 8
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25467 | 18721 | 2018-09-26 09:41:56 | 25467 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: According to the importance of micro-algae in aquatic feed such as fish in this study have been investigated Preparation of useful algae powder or concentrate for breeding silver carp , their impact on the growth of silver carp , rate per unit area and estimate n economic Development. Different algal species were isolated from hydrothermal fish farms, then were purified and mass culture . The next step microalgae were dried and powdered by spray dryer and were examined the fish feeding on them. During this study, 6 species of chlorophyt( Scenedesmus obliquus, Scenedesmus acuminatus, Chlorella vulgaris, Pediastrum boryanum, Pandorina morum, Ankistrodesmus falcatus) ,3 species of cyanophyta ( Anabaena flosaquae, Oscillatoria agardhi and Spirulina platensis) and 1 species of Bacillariophta ( Cyclotella meneghiniana were isolated from.Green algae and Blue -green algae were cultured in Zaindr medium, diatoms were cultured in Zaindr medium but with water of Anzali logoon and also in F2 medium with artificial sea water and spirulina was cultured in Zarouk medium. Microalgae were cultures then concentrated.Then the impact was examined on fish silver carp 2 to 3 grams. The results showed that Cyclotella has a greater role in the growth of silver carp and Anabaena floes aquae and Spirulina platensis tend to growth less than cyclotella. Scenedesmus obliquus and Scenedesmus acuminatus were respectively next algae that showed the greatest impact on fish growth. Scenedesmus obliquus feed rate was greater than any other algae for Daphnia.
    Keywords: Aquaculture ; Iran ; Silver carp ; Algal concentrate ; Micro-algae ; Scenedesmus obliquus ; Scenedesmus acuminatus ; Chlorella vulgaris ; Pediastrum boryanum ; Pandorina morum ; Ankistrodesmus falcatus ; Spirulina platensis ; Oscillatoria agardhi ; Cyclotella meneghiniana ; Preparation ; Feed
    Repository Name: AquaDocs
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  • 9
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25235 | 18721 | 2018-09-07 06:41:04 | 25235 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Goal of project implementation was survey of biological roles of Northern pike, Esox lucius, in decreasing of Coarse fish and other un economics organisms in carps ponds and also increasing of final crops in hectare and determination of Suitable ration of pike in ponds. This project implemented by 5 treatment with 3 replicates for every ones in 2 years. The pike density was 200, 350, 500 and 650 fish individuals per hectare for 1, 2, 3 and 4 treatment, respectively, and fifth treatment was without pike. The treatments of 1 and 3 conducted in first year and other in second year of test period. At first, about 26 brooders Caught from Anzali Lagoon, and reproduced by artificial and semi - natural methods in Sefidroud Fisheries Research Station in 12–14 °c temperature. Produced larvae reared in earthen ponds and fries reached to weight of 10 -12 g after 45 days. Mean weight, length and survival rates of fish was 12.27 and 32.5 g, 11.44 and 15.83 cm, and 40 and 21% after 45 and 60 days, respectively. The carp density in ponds was 3500 fish individuals per hectare and stocking rates were 55 % silver carp, 20 % common carp, 10 % bighead carp and 15 % grass carp. In throughout of the experiment period, physico– chemical factors measured and plankton (phyto and zoo), benthos, un wanted fish and other organisms investigated. The biomass of unwanted fishes reduced to 76/81 and 60/6 % in first and second year of experiments respectively. The results showed that Esox lucius had effect in increasing of objective fish produce about 17.9 % and 3.9 % in first and second period of project implementation respectively. The average of fish produce in pond unit (2400 m^2)was 842 kg (3508 kg/ha ), 825 kg (3439 kg/ha) and 776 kg (3232 kg/ha) in first , second and control treatments first year of test period , and also in second year was 865 kg (3603 kg/ha), 877 kg (3652 kg/ha) and 848 kg (3531 kg/ha) in second , fourth and control treatments , respectively. A significant different found in final weight mean of common carp between treatments and Control ponds (P 〈0/05), as the weight of common carp in treatments ponds was 220 % in first year and 191 % in second year more than control pond. In end of the trial, the mean weight of Esox lucius was 265 ± 74, 276 ± 104, 159 ± 33 and 265 ± 89 g in 1, 2, 3 and 4 treatment, respectively. Survival rate of Northern pike in 1 to 4 treatment was 43.8, 55.2, 40 and 35.6 %, respectively.
    Keywords: Aquaculture ; Iran ; Sefidroud River ; Polyculture ; Pike ; Esox lucius ; Carp ; Survey ; Organisms ; Density ; Temperature ; Larvae ; Earthen ponds ; Plankton ; Survival rate
    Repository Name: AquaDocs
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  • 10
    Publication Date: 2021-07-16
    Description: Caspian trout (Salmo trutta caspius ) is one of the migratory fish species which lives and feeds in the Caspian Sea .This species distributed in south and western of the Caspian Sea . In order to rehabilitation of the Caspian trout in western area, the operation of the project started from the beginning of the fishing season of the 20112012. Specimens of the Caspian trout obtained from beach seine cooperatives and have been transferred to the fish farm in Asalem region .Totally, 44 specimens of the Caspian trout has been caught in two month period .Most of the fishes was from Kargan roud river . The mean length and weight of the brood fishes were 67.7 cm and 3122.7 g respectively .As at present Shilat uses just the broods of the Tonekaboon region for restocking of this species ,we recommend to use the broods of the western region separately for rehabilitation of the stocks of this region .
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Guilan Province ; Karganroud river ; Caspian trout ; Salmo trutta caspius ; Broods ; Rehabilitation ; Broodstock ; Migratory fish ; Species ; Specimens
    Repository Name: AquaDocs
    Type: monograph
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