ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 156 (1991), S. 129-134 
    ISSN: 1432-072X
    Keywords: δ-Aminolevulinic acid synthase ; Bacteriochlorophyll ; Promoter activity ; Oxygen regulation ; Rhodobacter capsulatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The promoter of the Rhodobacter capsulatus hemA gene, coding for the enzyme δ-aminolevulinic acid synthase (ALAS), was identified by trans-complementation of a δ-aminolevulinic acid (ALA)-dependent mutant and found to be located within a 170 bp region proximal to the hemA gene. The activity of the hemA promoter was demonstrated by lacZ fusion and in vitro transcription-translation. An open reading frame (ORFX) was found downstream of hemA. The activity of the hemA promoter, but not that of the ORFX promoter, increased when oxygen tension was lowered in the culture. Deletions upstream of the hemA promoter region did not affect ALAS activity and formation of pigment-protein complexes in R. capsulatus.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 149 (1987), S. 12-18 
    ISSN: 1432-072X
    Keywords: Oxidase regulation ; Cytochrome c oxidase ; Cytochrome c 2 ; Regulatory DNA sequence ; Rhodobacter capsulatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to distinguish between the regulatory effects of oxygen tension and light intensity on cytochrome c oxidase protein and enzymatic activity cells of Rhodobacter capsulatus were shifted from phototrophic (anaerobic, light) growth to aerobic-light, aerobic-dark and to anaerobic-dark conditions, respectively. During shift-experiments the formation of oxidase protein and regulation of oxidase activity was followed by immunological and enzymatic means. The results support the idea, that the formation of oxidase protein is regulated by oxygen tension and light intensity changes, whereas the regulation of oxidase activity seems only to be correlated to the oxygen tension. A DNA sequence involved in the oxygen-dependent regulation of cytochrome oxidase could be identified in the regulation-deficient oxidase mutant H41 of R. capsulatus. Immunological investigations of cytochrome c 2 from mutant H41 demonstrated at the same time the participation of the c 2-polypeptide in the regulation of cytochrome c oxidase.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1432-072X
    Keywords: Protein phosphorylation ; Antenna protein ; Rhodobacter capsulatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Labelling of Rhodobacter capsulatus cells with (32P)Pi in a phototrophic culture results in phosphorylation of a membrane-bound polypeptide identified as the α subunit of the LHI antenna complex of the photosynthetic apparatus. Phosphorylation of the same polypeptide was also observed by incubation of chromatophores with (32P)ATP or under conditions of photophosphorylation with ADP and (32P)Pi. The identity of the phosphorylated LHI-α subunit was demonstrated by N-terminal protein sequencing of the phosphorylated polypeptide and by failure of labelling in LHI-defective mutants. Pre-aeration of the samples or addition of the oxidant potassium ferrcyanide stimulated the kinase activity whereas the presence of soluble cytoplasmic proteins impaired phosphorylation in an in vitro assay. No effect resulted from addition of reductants to the assay medium. The results indicate the presence of a membrane-bound protein kinase in R. capsulatus that phosphorylates the α subunit of the LHI antenna complex under redox control.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 0014-5793
    Keywords: LHI α polypeptide ; Membrane insertion ; Rhodobacter capsulatus ; Signal peptide ; Site-directed mutagenesis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1573-5079
    Keywords: light-harvesting complex I ; Rhodobacter capsulatus ; chaperone DnaK ; GroEL ; membrane insertion ; cell-free translation system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transcripts of the genes pufBA, pufB or pufA from Rhodobacter capsulatus were translated in a cell-free system of R. capsulatus. The incorporation of the nascent polypeptides LHIα and β in various types of membranes and the assembly of the light-harvesting (LH) complex I (B870) were investigated. The highest rate of stable incorporation of LHIα and β into the membrane was observed with membranes from the wild type strain grown under chemotrophic conditions. Addition of membranes from cells defective in biosynthesis of pigment-binding proteins resulted in a less efficient or less stable incorporation of LHIαβ. The single polypeptides LHIα or β were synthesized and inserted into the membrane but were extractable to a higher percentage by 6 M urea than the pairwise inserted LHI polypeptides. If the ribosomes and the S135 extract were depleted of DnaK the rate of synthesis of both polypeptides, LHIα and β, was strongly reduced. Removal of GroEL from the cell-free system did not impair the synthesis and membrane association of both proteins, but affected the stable insertion. A high percentage of the LHIαβ polypeptides became extractable by 6 M urea if the cell-free system was depleted of GroEL. Addition of GroEL to the cell-free system restored the capacity of stable insertion of both proteins into the membrane. GroEL interacted with LHIα and β before membrane targeting as shown by immunological means. A protein fraction, which can be removed from the membrane with a low-salt buffer, supported the effective and stable incorporation of LHIαβ into the membrane. It is concluded that the assembly of the LHI complex in the membrane system of R. capsulatus is a multistep process guided and supported by polypeptides located in the cytoplasm and in the membrane. In the cell-free in vitro system not only the correct insertion of the LHI polypeptides but also an assembly with bacteriochlorophyll was observed. BChl was synthesized from δ-amino levulinate in the cell free system.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1617-4623
    Keywords: hemA ; δ-Aminolevulinic acid synthase ; Tetrapyrrole biosynthesis ; Phototrophic bacteria ; Rhodobacter capsulatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The Rhodobacter capsulatus hemA gene, coding for the enzyme δ-aminolevulinic acid synthase (ALAS), was isolated from a genome bank by hybridization with a hemT probe from Rhodobacter sphaeroides. Subcloning of the initial 3.9 kb HindIII fragment allowed the isolation of a 2.5 kb HindIII-BglII fragment which was able to complement the δ-aminolevulinic acid-requiring (ALA-requiring) Escherichia coli mutant SHSP19. DNA sequencing revealed an open reading frame coding for a protein with 401 amino acids which displayed similarity to the amino acid sequences of other known ALASs. However, no resemblance was seen to the HemA protein of E. coli K12. Based on the sequence data, an ALA-requiring mutant strain of R. capsulatus was constructed by site-directed insertion mutagenesis. Introduction of a plasmid, containing the hemA gene of R. capsulatus on the 3.9 kb HindIII fragment, restored ALA-independent growth of the mutant indicating that there is only one gene for ALA biosynthesis in R. capsulatus. Transfer of the R′ factor pRPS404 and hybridization analysis revealed that the ALAS gene is not located within the major photosynthetic gene cluster.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 7
    Publication Date: 2021-04-21
    Description: Ice shelves around Antarctica can provide back stress for outlet glaciers and control ice sheet mass loss. They often contain narrow bands of thin ice termed ice shelf channels. Ice shelf channel morphology can be interpreted through surface depressions and exhibits junctions and deflections from flowlines. Using ice flow modeling and radar, we investigate ice shelf channels in the Roi Baudouin Ice Shelf. These are aligned obliquely to the prevailing easterly winds. In the shallow radar stratigraphy, syncline and anticline stacks occur beneath the upwind and downwind side, respectively. The structures are horizontally and vertically coherent, except near an ice shelf channel junction where patterns change structurally with depth. Deeper layers truncate near basal incisions. Using ice flow modeling, we show that the stratigraphy is ∼9 times more sensitive to atmospheric variability than to oceanic variability. This is due to the continual adjustment toward flotation. We propose that syncline‐anticline pairs in the shallow stratigraphy are caused by preferential snow deposition on the windward side and wind erosion at the downwind side. This drives downwind deflection of ice shelf channels of several meters per year. The depth variable structures indicate formation of an ice shelf channel junction by basal melting. We conclude that many ice shelf channels are seeded at the grounding line. Their morphology farther seaward is shaped on different length scales by ice dynamics, the ocean, and the atmosphere. These processes act on finer (subkilometer) scales than are captured by most ice, atmosphere, and ocean models, yet the dynamics of ice shelf channels may have broader implications for ice shelf stability.
    Description: Plain Language Summary Ice flows from Antarctica's interior toward the coast. At the contact point between ice and ocean, the ice becomes afloat and forms fast‐flowing ice shelves. Snowfall continuously accumulates at the ice shelf surface, and at the ice shelf bottom the relatively warm ocean water can melt ice from below. Ice shelves sometimes exhibit a network of surface depressions resembling a river network. At the base, the depressions are accompanied by large incisions termed ice shelf channels. Using radar as a tool for echolocation, we investigate how the shape of this network is formed. We find that snow preferentially collects in the upwind side of the surface depressions. This makes ice shelf channels move to the downwind side. We also find that ice shelf channels can form junctions through localized ocean melting. This is important because it helps us to better understand how the Antarctic ice sheet interacts with the surrounding ocean.
    Description: Key Points The radar stratigraphy in ice shelves is 9 times more sensitive to variability in snow deposition than to variability in basal melting Some ice shelf channels at Roi Baudouin Ice Shelf deflect from flowlines; the radar stratigraphy reflects related processes Variable snow deposition causes slow deflection, and basal melting can form ice shelf channel junctions far from the grounding line
    Description: InBev Baillet Latour Antarctica Fellowship
    Description: Belgian Science Policy Office http://dx.doi.org/10.13039/501100002749
    Description: DFG Emmy Noether
    Description: Academy of Finland http://dx.doi.org/10.13039/501100002341
    Description: Deutsche Forschungsgemeinschaft (DFG) http://dx.doi.org/10.13039/501100001659
    Description: “Antarctic Research with comparative investigations in Arctic ice areas”
    Description: Academy of Finnland
    Description: Belgium Science Policy Office
    Description: Deutsche Forschungsgemeinschaft (DFG) http://dx.doi.org/10.13039/501100001659
    Description: InBev Baillet Latour Antarctica Fellowship
    Keywords: 551 ; glaciology ; geophysics ; Antarctica ; ice shelves ; ice‐ocean interaction ; ice shelf channels
    Type: article
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 8
    Publication Date: 2021-07-03
    Description: The shape of ice shelf cavities are a major source of uncertainty in understanding ice‐ocean interactions. This limits assessments of the response of the Antarctic ice sheets to climate change. Here we use vibroseis seismic reflection surveys to map the bathymetry beneath the Ekström Ice Shelf, Dronning Maud Land. The new bathymetry reveals an inland‐sloping trough, reaching depths of 1,100 m below sea level, near the current grounding line, which we attribute to erosion by palaeo‐ice streams. The trough does not cross‐cut the outer parts of the continental shelf. Conductivity‐temperature‐depth profiles within the ice shelf cavity reveal the presence of cold water at shallower depths and tidal mixing at the ice shelf margins. It is unknown if warm water can access the trough. The new bathymetry is thought to be representative of many ice shelves in Dronning Maud Land, which together regulate the ice loss from a substantial area of East Antarctica.
    Description: Plain Language Summary: Antarctica is surrounded by floating ice shelves, which play a crucial role in regulating the flow of ice from the continent into the oceans. The ice shelves are susceptible to melting from warm ocean waters beneath them. In order to better understand the melting, knowledge of the shape and depth of the ocean cavity beneath ice shelves is crucial. In this study, we present new measurements of the sea floor depth beneath Ekström Ice Shelf in East Antarctica. The measurements reveal a much deeper sea floor than previously known. We discuss the implications of this for access of warm ocean waters, which can melt the base of the ice shelf and discuss how the observed sea floor features were formed by historical ice flow regimes. Although Ekström Ice Shelf is relatively small, the geometry described here is thought to be representative of the topography beneath many ice shelves in this region, which together regulate the ice loss from a substantial area of East Antarctica.
    Description: Key Points: Vibroseis seismic surveys used to map the ice shelf cavity beneath Ekström Ice Shelf in Antarctica. Deep trough with transverse sills and overdeepenings provide evidence of past ice streaming and retreat. Two ocean circulation regimes inferred in the shallow and deep parts of the cavity.
    Description: Belgian Science Policy Contract
    Description: Deutsche Forschungsgemeinschaft (DFG) http://dx.doi.org/10.13039/501100001659
    Description: DFG Cost S2S project
    Description: RD http://dx.doi.org/10.13039/100009936
    Keywords: 550.28 ; 551.31 ; Ice shelf ; Antarctica ; Bathymetry ; Ice‐Ocean Interaction ; Ice dynamics ; Seismics
    Type: article
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 9
    Publication Date: 2024-01-11
    Description: Two ice-dynamic transitions of the Antarctic ice sheet – the boundary of grounded ice features and the freelyfloating boundary – are mapped at 15-m resolution by participants of the International Polar Year project ASAID using customized software combining Landsat-7 imagery and ICESat/GLAS laser altimetry. The grounded ice boundary is 53 610 km long; 74% abuts to floating ice shelves or outlet glaciers, 19% is adjacent to open or sea-ice covered ocean, and 7% of the boundary ice terminates on land. The freelyfloating boundary, called here the hydrostatic line, is the most landward position on ice shelves that expresses the full amplitude of oscillating ocean tides. It extends 27 521 km and is discontinuous. Positional (one-sigma) accuracies of the grounded ice boundary vary an order of magnitude ranging from ±52m for the land and open-ocean terminating segments to ±502m for the outlet glaciers. The hydrostatic line is less well positioned with errors over 2 km. Elevations along each line are selected from 6 candidate digital elevation models based on their agreement with ICESat elevation values and surface shape inferred from the Landsat imagery. Elevations along the hydrostatic line are converted to ice thicknesses by applying a firn-correction factor and a flotation criterion. BEDMAP-compiled data and other airborne data are compared to the ASAID elevations and ice thicknesses to arrive at quantitative (one-sigma) uncertainties of surface elevations of ±3.6, ±9.6, ±11.4, ±30 and ±100m for five ASAID-assigned confidence levels. Over one-half of the surface elevations along the grounded ice boundary and over one-third of the hydrostatic line elevations are ranked in the highest two confidence categories. A comparison between ASAID-calculated ice shelf thicknesses and BEDMAP-compiled data indicate a thin-ice bias of 41.2±71.3m for the ASAID ice thicknesses. The relationship between the seaward offset of the hydrostatic line from the grounded ice boundary only weakly matches a prediction based on beam theory. The mapped products along with the customized software to generate them and a variety of intermediate products are available from the National Snow and Ice Data Center.
    Description: Published
    Description: 569-588
    Description: 3.8. Geofisica per l'ambiente
    Description: JCR Journal
    Description: open
    Keywords: Antarctica ; ice sheet ; photoclinometry ; freely-floating boundaries ; 02. Cryosphere::02.02. Glaciers::02.02.05. Ice dynamics ; 02. Cryosphere::02.02. Glaciers::02.02.06. Mass balance ; 02. Cryosphere::02.02. Glaciers::02.02.07. Ocean/ice interaction
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...