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  • 1
    Electronic Resource
    Electronic Resource
    Studies on the regulation of assimilatory nitrate reductase in Ankistrodesmus braunii (1977)
    Springer
    Planta 137 (1977), S. 231-234 
    Details
    ISSN: 1432-2048
    Keywords: Ankistrodesmus ; Diaphorase ; Enzyme inactivation ; Nitrate reductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the green alga Ankistrodesmus braunii, all the activities associated with the nitrate reductase complex (i.e., NAD(P)H-nitrate reductase, NAD(P)H-cytochrome c reductase and FMNH2-or MVH-nitrate reductase) are nutritionally repressed by ammonia or methylamine. Besides, ammonia or methylamine promote in vivo the reversible inactivation of nitrate reductase, but not of NAD(P)H-cytochrome c reductase. Subsequent removal of the inactivating agent from the medium causes reactivation of the inactive enzyme. Menadione has a striking stimulation on the in vivo reactivation of the inactive enzyme. The nitrate reductase activities, but not the diaphorase activity, can be inactivated in vitro by preincubating a partially purified enzyme preparation with NADH or NADPH. ADP, in the presence of Mg2+, presents a cooperative effect with NADH in the in vitro inactivation of nitrate reductase. This effect appears to be maximum at a concentration of ADP equimolecular with that of NADH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388155
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  • 2
    Electronic Resource
    Electronic Resource
    Large-scale cultivation of Solanum chrysotrichum cells: Production of the antifungal saponin SC-1 in 10-l airlift bioreactors (1997)
    Springer
    Plant cell reports 16 (1997), S. 653-656 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsSolanum chrysotrichum ; Cell suspension culture ; Spirostanol saponins ; Antimycotic ; Large-scale culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of two different cell lines: ccvx (cotyledon derived) and ccvz (hypocotyl derived) of Solanum chrysotrichum were cultivated in 10-l airlift bioreactors for the production of the human antimycotic compound SC-1. When using 3 g l–1 dry weight inoculum in a batch culture, higher levels of biomass were achieved with the ccvx cell line (14.6 g l–1) than with ccvz (7.7 g l–1), resulting in 23 and 12 mg g–1 of SC-1 after 17 days in culture for ccvx and ccvz, respectively. The maximum productivity of SC-1 in bioreactors was 0.025 g l–1 day–1 after 9 days in culture. When using a draw-fill mode, the productivity increased by 60% to a value of 0.041 g l–1 day–1, 4 days after 50% of the cell suspension was removed and replaced with fresh medium. This latter bioreactor system is a feasible alternative for the production of the antimycotic metabolite of S. chrysotrichum on a large scale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050296
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  • 3
    Electronic Resource
    Electronic Resource
    Large-scale cultivation ofSolanum chrysotrichum cells: Production of the antifungal saponin SC-1 in 10-I airlift bioreactors (1997)
    Springer
    Plant cell reports 16 (1997), S. 653-656 
    Details
    ISSN: 1432-203X
    Keywords: Solanum chrysotrichum ; Cell suspension culture ; Spirostanol saponins ; Antimycotic ; Large-scale culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of two different cell lines:ccvx (cotyledon derived) andccvz (hypocotyl derived) ofSolanum chrysotrichum were cultivated in 10-1 airlift bioreactors for the production of the human antimycotic compound SC-1. When using 3 g l-1 dry weight inoculum in a batch culture, higher levels of biomass were achieved with theccvx cell line (14.6 g l-1) than withccvz (7.7 g l-1), resulting in 23 and 12 mg g-1 of SC-1 after 17 days in culture forccvx andccvz, respectively. The maximum productivity of SC-1 in bioreactors was 0.025 g l-1 day-1 after 9 days in culture. When using a draw-fill mode, the productivity increased by 60% to a value of 0.041 g l-1 day, 4 days after 50% of the cell suspension was removed and replaced with fresh medium. This latter bioreactor system is a feasible alternative for the production of the antimycotic metabolite ofS. chrysotrichum on a large scale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275509
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    Paper (German National Licenses)
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