Publication Date:
2013-07-24
Description:
The dynamic nature of gene expression enables cellular programming, homeostasis and environmental adaptation in living systems. Dissection of causal gene functions in cellular and organismal processes therefore necessitates approaches that enable spatially and temporally precise modulation of gene expression. Recently, a variety of microbial and plant-derived light-sensitive proteins have been engineered as optogenetic actuators, enabling high-precision spatiotemporal control of many cellular functions. However, versatile and robust technologies that enable optical modulation of transcription in the mammalian endogenous genome remain elusive. Here we describe the development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana. LITEs do not require additional exogenous chemical cofactors, are easily customized to target many endogenous genomic loci, and can be activated within minutes with reversibility. LITEs can be packaged into viral vectors and genetically targeted to probe specific cell populations. We have applied this system in primary mouse neurons, as well as in the brain of freely behaving mice in vivo to mediate reversible modulation of mammalian endogenous gene expression as well as targeted epigenetic chromatin modifications. The LITE system establishes a novel mode of optogenetic control of endogenous cellular processes and enables direct testing of the causal roles of genetic and epigenetic regulation in normal biological processes and disease states.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856241/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856241/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Konermann, Silvana -- Brigham, Mark D -- Trevino, Alexandro E -- Hsu, Patrick D -- Heidenreich, Matthias -- Cong, Le -- Platt, Randall J -- Scott, David A -- Church, George M -- Zhang, Feng -- DP1 MH100706/MH/NIMH NIH HHS/ -- DP1-MH100706/DP/NCCDPHP CDC HHS/ -- P50-HG005550/HG/NHGRI NIH HHS/ -- R01 DK097768/DK/NIDDK NIH HHS/ -- R01 NS073124/NS/NINDS NIH HHS/ -- R01-NS073124/NS/NINDS NIH HHS/ -- England -- Nature. 2013 Aug 22;500(7463):472-6. doi: 10.1038/nature12466. Epub 2013 Aug 23.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23877069" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Arabidopsis Proteins/metabolism
;
Basic Helix-Loop-Helix Transcription Factors/metabolism
;
Cells, Cultured
;
Chromatin/genetics/radiation effects
;
Cryptochromes/metabolism
;
Epigenesis, Genetic/*genetics/*radiation effects
;
Gene Expression Regulation/genetics/*radiation effects
;
Genetic Vectors/genetics
;
*Light
;
Male
;
Mice
;
Mice, Inbred C57BL
;
Neurons/metabolism/radiation effects
;
Optogenetics/*methods
;
Time Factors
;
Transcription, Genetic/genetics/*radiation effects
;
Two-Hybrid System Techniques
;
Wakefulness
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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