Publikationsdatum:
2014-07-22
Beschreibung:
The c-myc proto-oncogene product, Myc, is a transcription factor that binds thousands of genomic loci. Recent work suggested that rather than up- and downregulating selected groups of genes, Myc targets all active promoters and enhancers in the genome (a phenomenon termed 'invasion') and acts as a general amplifier of transcription. However, the available data did not readily discriminate between direct and indirect effects of Myc on RNA biogenesis. We addressed this issue with genome-wide chromatin immunoprecipitation and RNA expression profiles during B-cell lymphomagenesis in mice, in cultured B cells and fibroblasts. Consistent with long-standing observations, we detected general increases in total RNA or messenger RNA copies per cell (hereby termed 'amplification') when comparing actively proliferating cells with control quiescent cells: this was true whether cells were stimulated by mitogens (requiring endogenous Myc for a proliferative response) or by deregulated, oncogenic Myc activity. RNA amplification and promoter/enhancer invasion by Myc were separable phenomena that could occur without one another. Moreover, whether or not associated with RNA amplification, Myc drove the differential expression of distinct subsets of target genes. Hence, although having the potential to interact with all active or poised regulatory elements in the genome, Myc does not directly act as a global transcriptional amplifier. Instead, our results indicate that Myc activates and represses transcription of discrete gene sets, leading to changes in cellular state that can in turn feed back on global RNA production and turnover.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110711/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110711/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sabo, Arianna -- Kress, Theresia R -- Pelizzola, Mattia -- de Pretis, Stefano -- Gorski, Marcin M -- Tesi, Alessandra -- Morelli, Marco J -- Bora, Pranami -- Doni, Mirko -- Verrecchia, Alessandro -- Tonelli, Claudia -- Faga, Giovanni -- Bianchi, Valerio -- Ronchi, Alberto -- Low, Diana -- Muller, Heiko -- Guccione, Ernesto -- Campaner, Stefano -- Amati, Bruno -- 10-0245/Worldwide Cancer Research/United Kingdom -- 268671/European Research Council/International -- England -- Nature. 2014 Jul 24;511(7510):488-92. doi: 10.1038/nature13537. Epub 2014 Jul 9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉1] Center for Genomic Science of IIT@SEMM, Fondazione Istituto Italiano di Tecnologia (IIT), Via Adamello 16, 20139 Milan, Italy [2] Department of Experimental Oncology, European Institute of Oncology (IEO), Via Adamello 16, 20139 Milan, Italy [3]. ; 1] Center for Genomic Science of IIT@SEMM, Fondazione Istituto Italiano di Tecnologia (IIT), Via Adamello 16, 20139 Milan, Italy [2]. ; Center for Genomic Science of IIT@SEMM, Fondazione Istituto Italiano di Tecnologia (IIT), Via Adamello 16, 20139 Milan, Italy. ; Department of Experimental Oncology, European Institute of Oncology (IEO), Via Adamello 16, 20139 Milan, Italy. ; Institute of Molecular and Cell Biology, Singapore 138673, Singapore. ; 1] Center for Genomic Science of IIT@SEMM, Fondazione Istituto Italiano di Tecnologia (IIT), Via Adamello 16, 20139 Milan, Italy [2] Department of Experimental Oncology, European Institute of Oncology (IEO), Via Adamello 16, 20139 Milan, Italy.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25043028" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Animals
;
B-Lymphocytes/metabolism/pathology
;
*Cell Proliferation
;
Cell Transformation, Neoplastic/*genetics/pathology
;
Chromatin/genetics/metabolism
;
Chromatin Immunoprecipitation
;
Disease Progression
;
Down-Regulation/genetics
;
Female
;
Fibroblasts/cytology/metabolism
;
Gene Expression Profiling
;
*Gene Expression Regulation, Neoplastic/genetics
;
Genome/genetics
;
Lymphoma, B-Cell/*genetics/metabolism/*pathology
;
Male
;
Mice
;
Mitogens/pharmacology
;
Promoter Regions, Genetic/genetics
;
Proto-Oncogene Proteins c-myc/genetics/*metabolism
;
RNA, Messenger/biosynthesis/genetics/metabolism
;
Transcription Factors/metabolism
;
*Transcription, Genetic/genetics
;
Up-Regulation/genetics
Print ISSN:
0028-0836
Digitale ISSN:
1476-4687
Thema:
Biologie
,
Chemie und Pharmazie
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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