Publication Date:
2000-09-23
Description:
When DNA replication is inhibited during the synthesis (S) phase of the cell cycle, a signaling pathway (checkpoint) is activated that serves to prevent mitosis from initiating before completion of replication. This replication checkpoint acts by down-regulating the activity of the mitotic inducer cdc2-cyclin B. Here, we report the relation between chromatin structure and induction of the replication checkpoint. Chromatin was competent to initiate a checkpoint response only after the DNA was unwound and DNA polymerase alpha had been loaded. Checkpoint induction did not require new DNA synthesis on the unwound template strand but did require RNA primer synthesis by primase. These findings identify the RNA portion of the primer as an important component of the signal that activates the replication checkpoint.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Michael, W M -- Ott, R -- Fanning, E -- Newport, J -- 52948/PHS HHS/ -- R01GM33523-16/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2000 Sep 22;289(5487):2133-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, University of California, San Diego, La Jolla, CA 92093-0349, USA. matt@mcb.harvard.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11000117" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Aphidicolin/pharmacology
;
*CDC2-CDC28 Kinases
;
Carrier Proteins/metabolism
;
Chromatin/*metabolism
;
Cyclin E/metabolism
;
Cyclin-Dependent Kinase 2
;
Cyclin-Dependent Kinases/metabolism
;
DNA Helicases/metabolism
;
DNA Polymerase I/antagonists & inhibitors/metabolism
;
DNA Primase/*metabolism
;
*DNA Replication/drug effects
;
DNA, Single-Stranded/metabolism
;
DNA-Binding Proteins/metabolism
;
Mitosis
;
Nuclear Proteins/metabolism
;
Phosphorylation
;
Protein Kinases/metabolism
;
Protein-Serine-Threonine Kinases/metabolism
;
RNA/*biosynthesis
;
Recombinant Proteins/metabolism
;
S Phase
;
*Saccharomyces cerevisiae Proteins
;
Signal Transduction
;
Xenopus
;
Xenopus Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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