ISSN:
0935-6304
Schlagwort(e):
Supercritical fluid chromatography, SFC
;
Fused silica capillary columns
;
Low temperature analysis
;
Glycerides
;
Trinervonin
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
The separation of mixed mono-, di-, and triglycerides by capillary supercritical fluid chromatography is described. The separations were performed at low operating temperatures, using a carbon dioxide mobile phase, a conventional flame ionization detector, and two different stationary phases, DB-5 (95% dimethyl-(5%)-diphenylpolysiloxane) and DB-225 (50% cyanopropyl-methyl-(50%)-methylphenylpolysiloxane). Because the separations were performed at low operating temperatures, no thermal degradation of the glycerides was observed. Even under these mild operating conditions, trinervonin, a triglyceride having a molecular weight over 1100 amu, was readily eluted. When rapid pressure programming of the carbon dioxide mobile phase was employed, trinervonin was eluted in less than two minutes. On DB-5, the mono-, di-, and triglycerides eluted in order of increasing molecular weight. A graph of t′r (on DB-5) versus molecular weight is linear. When DB-225 was used as the stationary phase, triglycerides eluted in the order of increasing unsaturation. Thus, the order of elution on DB-225 was tristearin, triolein, trilinolein, and trilinolenin.
Zusätzliches Material:
4 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/jhrc.1240080605
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