ISSN:
0935-6304
Keywords:
HPLC
;
Injection valves
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
In liquid chromatography with “low-dispersion methods”, there is an increasing need to reproducibly inject nanoliter sample volumes. Low-dispersion methods produce very narrow peaks because of short column length, narrow column bore, small particle packing, low particle surface area, open tubular configuration, or combinations of these parameters.This paper describes a new injector method, the “groove-injector” which involves simple plumbing changes for use of a conventional multi-port valve (8-ports or more) to inject sample volumes approximating a single groove in such a valve (e.g 30 nanoliters with aceptable reproducibility, ca. 8% RSD). In addition, by changing a resistor, volumes between 30, and over 2,000 nanoliters (nearly two orders of magnitude) can be injected with reproducibilities generally below 2% RSD. Different samples can be injected by using an autosampler.Compared to commonly used 4-port valves for nanoliter injections, multi-port valves have a number of advantages. Multiport valves generally are more commonly available and they are a better financial investment because of their versatility for column switching, sample enrichment, or variable volume injections. Previously, submicroliter (nanoliter) injections have not been possible with multi-port valves in as direct and simple a manner as described here.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240110131
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