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  • 1
    Electronic Resource
    Electronic Resource
    Evidence for an ammonium transport system in the N2-fixing phototrophic bacteriumRhodospirillum rubrum (1982)
    Springer
    Archives of microbiology 132 (1982), S. 79-81 
    Details
    ISSN: 1432-072X
    Keywords: Ammonium transport ; Regulation ; Rhodospirillum rubrum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An ammonium transport system in the phototrophic N2-fixing bacteriumRhodospirillum rubrum was characterized by using the uptake of14C-methylamine as a probe. Uptake showed saturation kinetics with an apparentK m =110 μM. It was competitively inhibited by ammonium (K i =7 μM). Uptake exhibited a narrow pH maximum around pH 7.0. Up to 200-fold gradients across the membrane were formed within 40–60 min. Gradient formation was inhibited by carbon starvation, azide or cyanide. Pre-accumulated methylamine was released by ammonium pulses to more than 80%, indicating only minor metabolization. The synthesis of the transport system was repressed by ammonium in high concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690822
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  • 2
    Electronic Resource
    Electronic Resource
    Regulatory aspects of inorganic nitrogen metabolism in the Rhodospirillaceae (1982)
    Springer
    Archives of microbiology 133 (1982), S. 239-241 
    Details
    ISSN: 1432-072X
    Keywords: Rhodsopirillaceae ; Ammonium transport ; Nitrogenase ; Nitrate reduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Regulatory aspects of the assimilation of inorganic nitrogen compounds (ammonia, nitrate, nitrogen) were studied in 12 strains belonging to the Rhodospirillaceae. All strains possessed an ammonium transport system, as demonstrated by 14C-methylammonium uptake. This uptake showed saturation kinetics (K m between 50–150 μM), and was competitively inhibited by ammonium (K i between 5–18 μM). The ammonium transport systems were repressed by ammonium in the growth medium. The nitrogenase activity of all strains was reversibly inhibited by ammonium (“switch-off”). This effect was not shown under nitrogen starvation conditions with the exception of some strains of Rhodopseudomonas capsulata, the nitrogenase of which was always susceptible to switch-off by ammonium. Assimilation of nitrate was confined to some strains of Rhodopseudomonas capsulata.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00415009
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  • 3
    Electronic Resource
    Electronic Resource
    Some properties of a Klebsiella pneumoniae ammonium transport negative mutant (Amt−) (1984)
    Springer
    Archives of microbiology 139 (1984), S. 245-247 
    Details
    ISSN: 1432-072X
    Keywords: Klebsiella pneumoniae ; Amt - mutant ; Ammonium excretion ; Ammonium transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The main property of an Amt- (ammonium transport negative) mutant of Klebsiella pneumoniae is its inability to accumulate NH 4 + intracellularly. When growing on nitrogen sources other than NH 4 + , the mutant constantly looses NH3 by diffusion. This loss results in poor growth. The NH3 excretion suggests the existence of a futile cycle (NH3 loss/NH 4 + reabsorption) in the wild type and possibly other bacterial strains, which do not constantly excrete NH3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402008
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  • 4
    Electronic Resource
    Electronic Resource
    Regulation of methylammonium transport inParacoccus denitrificans (1985)
    Springer
    Archives of microbiology 142 (1985), S. 285-288 
    Details
    ISSN: 1432-072X
    Keywords: Paracoccus denitrificans ; Methylammonium transport ; Ammonium transport ; Nitrogen control ; Carbon control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Depending on the growth conditionsParacoccus denitrificans synthesizes two different carriers mediating uptake of methylamine. When used as a nitrogen source, methylamine is transported via a NH 4 + carrier, and its transport is inhibited by NH 4 + but not by ethylamine. When used as a carbon source, methylamine is transported by a specific alkylamine carrier, and its transport is inhibited by ethylamine but not by NH 4 + . The NH 4 + carrier is under nitrogen control, the alkylamine carrier under carbon control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00693405
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