ISSN:
1432-072X
Keywords:
Ammonium metabolism
;
glnAntrBC
;
Nitrogenase regulation
;
Nitrogen control
;
Ammonia excretion
;
Amino acid utilization
;
Ntr
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Enterobacterial mutants defective in the nitrogen control regulatory system (Ntr) generally display a pleiotropic phenotype with regard to expression and regulation of several enzymes and transport systems involved in the assimilation of N sources. This report describes the isolation and characterization of similar pleiotropic mutants ofKlebsiella pneumoniae that cannot be complemented byntr genes. The strains excreted ammonia, were unable to grow on a number of N sources, and contained low glutamine:2-oxoglutarate amino transferase and normal, but unmodifiable glutamine synthetase activities and a nitrogenase level largely unaffected by ammonium, but still repressible by an amino acid mixture. Genetic studies suggested that this phenotype is due to overexpression of an unknown regulatory protein.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01682984
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