ISSN:
1573-5028
Keywords:
Aminoglycoside-3″-adenyltransferase
;
chimeric gene
;
Nicotiana tabacum
;
transgenic plant
;
spectinomycin resistance
;
streptomycin resistance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The bacterial gene aad A encodes the enzyme aminoglycoside-3″-adenyltransferase that confers resistance to spectinomycin and streptomycin in Escherichia coli. Chimeric genes have been constructed for expression in plants, and were introduced into Nicotiana tabacum by Agrobacterium binary transformation vectors. Spectinomycin or streptomycin in selective concentrations prevent greening of N. tabacum calli. Transgenic clones, however, formed green calli on selective media containing spectinomycin, streptomycin, or both drugs. Resistance was inherited as a dominant Mendelian trait in the seed progeny. Resistance conferred by the chimeric aad A gene can be used as a color marker similar to the resistance conferred by the streptomycin phosphotransferase gene to streptomycin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00018560
Permalink
