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Aminoglycoside-3″-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum

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Abstract

The bacterial gene aad A encodes the enzyme aminoglycoside-3″-adenyltransferase that confers resistance to spectinomycin and streptomycin in Escherichia coli. Chimeric genes have been constructed for expression in plants, and were introduced into Nicotiana tabacum by Agrobacterium binary transformation vectors. Spectinomycin or streptomycin in selective concentrations prevent greening of N. tabacum calli. Transgenic clones, however, formed green calli on selective media containing spectinomycin, streptomycin, or both drugs. Resistance was inherited as a dominant Mendelian trait in the seed progeny. Resistance conferred by the chimeric aad A gene can be used as a color marker similar to the resistance conferred by the streptomycin phosphotransferase gene to streptomycin.

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Author notes

  1. Zora Svab & Pal Maliga

    Present address: Waksman Institute, Rutgers, The State University of New Jersey, 08855-0759, Piscataway, NJ, USA

  2. Jonathan D. G. Jones

    Present address: The Sainsbury Laboratory, John Innes Institute, Colney Lane, NR4 7UH, Norwich, U.K.

Authors and Affiliations

  1. DNA Plant Technology Corporation, 6701 San Pablo Avenue, 94608, Oakland, CA, USA

    Zora Svab, Elisabeth C Harper, Jonathan D. G. Jones & Pal Maliga

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  1. Zora Svab
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  2. Elisabeth C Harper
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  3. Jonathan D. G. Jones
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  4. Pal Maliga
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Svab, Z., Harper, E.C., Jones, J.D.G. et al. Aminoglycoside-3″-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum . Plant Mol Biol 14, 197–205 (1990). https://doi.org/10.1007/BF00018560

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  • DOI: https://doi.org/10.1007/BF00018560

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