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  • Allium sativum L.  (1)
  • Androgenesis  (1)
  • Root segments  (1)
  • 1995-1999  (2)
  • 1955-1959
  • 1910-1914
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Schlagwörter
Verlag/Herausgeber
Erscheinungszeitraum
  • 1995-1999  (2)
  • 1955-1959
  • 1910-1914
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    Continuous callus production and regeneration of garlic (Allium sativum L.) using root segments from shoot tip-derived plantlets (1998)
    Springer
    Plant cell reports 17 (1998), S. 726-730 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key words Garlic ; Allium sativum L. ; Root segments ; Regeneration ; Picloram
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Root segments from shoot tip-derived plantlets of the garlic (Allium sativum L.) clones `DDR7099', `PI383819', and `Piacenza' were utilized as an explant source for continuous, friable callus production. The best callus production occurred on root segments initially cultured on medium with 4,5 μm 2,4-dichlorophenoxyacetic acid (2,4-D) for 8 weeks, then subcultured to medium with 4.7 μm 4-amino-3,5,6-trichloropicolinic acid (picloram) +0.49 μm 6-(γ-γ-dimethylallylamino)purine (2iP) for 8 weeks. Embryogenic, friable callus was transferred to liquid medium for 1 month and then transferred to solid regeneration medium for 14 weeks. The best shoot and root regeneration (85.3% and 35.8%, respectively) occurred on 4-month-old calli from the clone `DDR7099'. In all clones, regeneration rate decreased as callus age increased.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990050473
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Induction of diploid androgenetic and mitotic gynogenetic Nile tilapia (Oreochromis niloticus L.) (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 205-210 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Androgenesis ; Gynogenesis ; Tilapia Gene bank ; Chromosome manipulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Androgenesis is a potentially valuable technique for recovering fish from gene banks composed of cryopreserved sperm, developing inbred lines, and analyzing patterns of inheritance. The procedure for producing diploid organisms whose nuclear DNA is wholly of paternal origin is dependent on: (1) the denucleation of “host” eggs, and (2) the inhibition of the first mitotic division in order to double the haploid sperm chromosome complement following fertilization of host eggs. Denucleation of tilapia (Oreochromis niloticus L.) eggs was carried out using UV irradiation. Treatment durations of 5–8 min (total dose of 450–720 J/m2) produced acceptable yields of viable denucleated eggs [22.9±1.6% (±SE) of controls] as estimated by the survival of haploid androgenetic tilapia to 48 h post-fertilization. Successful mitotic inhibition was accomplished using a heat-shock of 42.5 °C for 3–4 min, applied at 2.5-min intervals from 22.5 to 30 min post-fertilization (mpf). The mean survival of androgenetic diploid fish to yolk-sac absorption for treatment groups varied from 0.4% to 5.3%, relative to the controls. Differences in the suceptibility of eggs from different females to UV irradiation were a significant factor in the overall yield of androgenetic diploids. Paternal effects did not significantly influence the androgenetic yield, suggesting that individual males would not be selected against. For comparative purposes mitotic gynogenetic “mitogyne” diploids were produced from UV-irradiated sperm. Mean survival to yolk-sac absorption varied from 0.5% to 10.64%, relative to controls. Similar optima for androgenetic and gynogenetic induction were found in the period 25–27.5 mpf (minutes post-fertilization). Induction treatments would appear to be operating on the same developmental events in both these techniques, and the results suggest that the UV irradiations used do relatively little damage to the eggs beyond nuclear inactivation. The results indicate that the production of androgenetic O. niloticus is possible on a consistent basis and that the application of this technique may be useful in quantitative and conservation genetics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222203
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
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