ISSN:
1573-9368
Keywords:
transformation vector
;
pBIN19
;
transgenic plants
;
T-DNA
;
Agrobacterium tumefaciens
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number inE. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01969123
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