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New plant promoter and enhancer testing vectors (1995)

Szabados, Làszlò ; Charrier, Bénédicte ; Kondorosi, Adam ; [et al.]

Springer

Molecular breeding 1 (1995), S. 419-423

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ISSN: 1572-9788

Keywords: binary vectors ; gus ; T-DNA ; Agrobacterium

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract We describe here a set of binary vectors suitable forAgrobacterium-mediated gene transfer and specially designed for studying plant promoters. These vectors are based on the use of thegus reporter gene, contain multiple unique restriction sites upstream of thegus gene, and minimal promoters for testing the effect of enhancers or activator elements. In addition, an intron-containinggus (uidA) gene was introduced into one of these vectors in order to examine reporter gene activity in tissues whereAgrobacterium contamination may be a problem or in transient expression assays.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01248419

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