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  • 1
    Electronic Resource
    Electronic Resource
    Fusion characteristics of plant protoplasts in electric fields (1985)
    Springer
    Planta 165 (1985), S. 205-216 
    Details
    ISSN: 1432-2048
    Keywords: Cell division ; Dielectrophoresis ; Electrofusion (electrical parameters) ; Heterokaryon ; Protoplast (mesophyll and suspension) ; Somatic hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The electrical parameters important in the fusion of plant protoplasts aligned dielectrophoretically in high-frequency alternating electric fields have been established. Protoplasts were aligned in an alternating electric field between two relatively distant (1 mm) electrodes, by dielectrophoresis induced by field inhomogeneities caused by the protoplasts themselves. This arrangement allowed ease of manipulations, large throughput and low loss of protoplasts. In analytical experiments, sufficiently large samples could be used to study pulse duration-fusion response relations at different pulse voltages for protoplasts of different species, tissues and size (mesophyll protoplasts of Solanum brevidens, Triticum aestivum, Hordeum vulgare; suspension-culture protoplasts of Nicotiana sylvestris, N. rustica, Datura innoxia and S. brevidens; root-tip protoplasts of Vicia faba, hypocotyl protoplasts of Brassica napus). The percentage of aligned protoplasts that fused increased with increasing pulse parameters (pulse duration; voltage) above a threshold that was dependant on pulse voltage. The maximum fusion values obtained depended on a number of factors including protoplast origin, size and chain length. Leaf mesophyll protoplasts fused much more readily than suspension-culture protoplasts. For both types, there was a correlation of size with fusion yield: large protoplasts tended to fuse more readily than small protoplasts. In short chains (≦five protoplasts), fusion frequency was lower, but the proportion of one-to-one products was greater than in long chains (≧ten protoplasts). In formation by electrofusion of heterokaryons between mesophyll and suspension-culture protoplasts, the fusion-frequency response curves reflected those of homofusion of mesophyll protoplasts rather than suspension-culture protoplasts. There was no apparent limitation to the fusion of the smallest mesophyll protoplast with the largest suspension-culture protoplasts. Based on these observations, it is possible to direct fusion towards a higher frequency of one-to-one (mesophyll/suspension) products by incorporating low densities of mesophyll protoplasts in high densities of suspensionculture protoplasts and by using a short fusion pulse. The viability of fusion products, assessed by staining with fluorescein diacetate, was not impaired by standard fusion conditions. On a preparative scale, heterokaryons (S. brevidens mesophyll-N. sylvestris or D. innoxia suspension-culture) were produced by electrofusion and cultured in liquid or embedded in agar, and were capable of wall formation, division and growth. It is concluded that the electrode arrangement described is more suitable for carrying out directed fusions of plant protoplasts than that employing closer electrodes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395043
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Somatic hybridization of amino acid analogue-resistant cell lines of potato (Solanum tuberosum L.) by electrofusion (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 451-458 
    Details
    ISSN: 1432-2242
    Keywords: Amino acid analogue-resistance ; Electrofusion ; Potato ; Solanum tuberosum L. ; Somatic hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intraspecific somatic hybridization between amino acid analogue-resistant cell lines of potato (Solanum tuberosum L.) has been carried out following electrofusion of protoplasts. In initial analytical electrofusion experiments (1 mm electrode separation) optimal fusion conditions were determined by changing the fusion medium (addition of Ca and/or spermine) and the electrical parameters. Subsequently, in large scale experiments, cell suspension protoplasts of aec-1, a variant resistant to AEC, were fused with the same type of protoplasts of 5mt-26 or 5mt-27, both variants resistant to 5MT and cross-resistant to 3 FT. After an extensive selection procedure only somatic hybrid lines of aec-1 + 5mt-26 were obtained. The resistance traits of aec-1 and 5mt-26 were expressed fully, indicating that the variant characters involved are transmitted dominantly. Quantitative examination of the free amino acid content revealed characteristics of both the parental cell lines in most of the somatic hybrids. However, initially selected double resistant colonies from fusions of aec-1 + 5mt-27 lines appeared not to be somatic hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262515
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  • 3
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    Exploratory Study of the Acoustic Performance of Piezoelectric Actuators (1989)
    In:  CASI
    Details
    Publication Date: 2018-06-05
    Description: The proposed ducted fan engine has prompted the need for increasingly lightweight and efficient noise control devices. Exploratory tests at the NASA Langley Research Center were conducted to evaluate three piezoelectric specimens as possible control transducers: a Polyvinylidene Flouride (PVDF) piezofilm sample and two composite samples of Lead Zirconate Titanate (PZT) rods embedded in fiberglass. The tests measured the acoustic output efficiency and evaluated the noise control characteristics when interacting with a primary sound source. The results showed that a PZT sample could diminish the reflected acoustic waves. However, the PZT acoustic output must increase by several orders of magnitude to qualify as a control transducer for the ducted fan engine.
    Keywords: Acoustics
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  • 4
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    Evaluation of Variable-Depth Liner Configurations for Increased Broadband Noise Reduction (2015)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: This paper explores the effects of variable-depth geometry on the amount of noise reduction that can be achieved with acoustic liners. Results for two variable-depth liners tested in the NASA Langley Grazing Flow Impedance Tube demonstrate significant broadband noise reduction. An impedance prediction model is combined with two propagation codes to predict corresponding sound pressure level profiles over the length of the Grazing Flow Impedance Tube. The comparison of measured and predicted sound pressure level profiles is sufficiently favorable to support use of these tools for investigation of a number of proposed variable-depth liner configurations. Predicted sound pressure level profiles for these proposed configurations reveal a number of interesting features. Liner orientation clearly affects the sound pressure level profile over the length of the liner, but the effect on the total attenuation is less pronounced. The axial extent of attenuation at an individual frequency continues well beyond the location where the liner depth is optimally tuned to the quarter-wavelength of that frequency. The sound pressure level profile is significantly affected by the way in which variable-depth segments are distributed over the length of the liner. Given the broadband noise reduction capability for these liner configurations, further development of impedance prediction models and propagation codes specifically tuned for this application is warranted.
    Keywords: Acoustics
    Type: NF1676L-20142 , AIAA/CEAS Aeroacoustics Conference; Jun 22, 2015 - Jun 26, 2015; Dallas, TX; United States
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  • 5
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    Impedance Eduction in Sound Fields With Peripherally Varying Liners and Flow (2015)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: A two-dimensional impedance eduction theory is extended to three-dimensional sound fields and peripherally varying duct liners. The approach is to first measure the acoustic pressure field at a series of flush-mounted wall microphones located around the periphery of the flow duct. The numerical solution for the acoustic pressure field at these microphones is also obtained by solving the three-dimensional convected Helmholtz equation using the finite element method. A quadratic objective function based on the difference between the measured and finite element solution is constructed and the unknown impedance function is obtained by minimizing this objective function. Impedance spectra educed for two uniform-structure liners (a wire-mesh and a conventional liner) and a hard-soft-hard peripherally varying liner (for which the soft segment is that of the conventional liner) are presented. Results are presented at three mean flow Mach numbers and fourteen sound source frequencies. The impedance spectra of the uniform-structure liners are also computed using a two-dimensional impedance eduction theory. The primary conclusions of the study are: 1) when measured data is used with the uniform-structure liners, the three-dimensional theory reproduces the same impedance spectra as the two-dimensional theory except for frequencies corresponding to very low or very high liner attenuation; and 2) good agreement between the educed impedance spectra of the uniform structure conventional liner and the soft segment of the peripherally varying liner is obtained.
    Keywords: Acoustics
    Type: NF1676L-19964 , AIAA Aviation 2015; Jun 22, 2015 - Jun 26, 2015; Dallas, TX; United States
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  • 6
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    Optimization of Microphone Locations for Acoustic Liner Impedance Eduction (2015)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: Two impedance eduction methods are explored for use with data acquired in the NASA Langley Grazing Flow Impedance Tube. The first is an indirect method based on the convected Helmholtz equation, and the second is a direct method based on the Kumaresan and Tufts algorithm. Synthesized no-flow data, with random jitter to represent measurement error, are used to evaluate a number of possible microphone locations. Statistical approaches are used to evaluate the suitability of each set of microphone locations. Given the computational resources required, small sample statistics are employed for the indirect method. Since the direct method is much less computationally intensive, a Monte Carlo approach is employed to gather its statistics. A comparison of results achieved with full and reduced sets of microphone locations is used to determine which sets of microphone locations are acceptable. For the indirect method, each array that includes microphones in all three regions (upstream and downstream hard wall sections, and liner test section) provides acceptable results, even when as few as eight microphones are employed. The best arrays employ microphones well away from the leading and trailing edges of the liner. The direct method is constrained to use microphones opposite the liner. Although a number of arrays are acceptable, the optimum set employs 14 microphones positioned well away from the leading and trailing edges of the liner. The selected sets of microphone locations are also evaluated with data measured for ceramic tubular and perforate-over-honeycomb liners at three flow conditions (Mach 0.0, 0.3, and 0.5). They compare favorably with results attained using all 53 microphone locations. Although different optimum microphone locations are selected for the two impedance eduction methods, there is significant overlap. Thus, the union of these two microphone arrays is preferred, as it supports usage of both methods. This array contains 3 microphones in the upstream hard wall section, 14 microphones opposite the liner, and 3 microphones in the downstream hard wall section.
    Keywords: Acoustics
    Type: NF1676L-20029 , AIAA/CEAS Aeroacoustics Conference; Jun 22, 2015 - Jun 26, 2015; Dallas, TX; United States
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  • 7
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    Impedance Eduction for Multisegment Liners (2018)
    In:  CASI
    Details
    Publication Date: 2019-07-20
    Description: This paper explores the validity of an indirect method for impedance eduction of multisegment liners. This is accomplished via results obtained with two uniform liners and one two-segment liner, where each segment is constructed to match the geometry of one of the uniform liners. Each uniform liner is evaluated using direct and indirect impedance eduction methods. An indirect impedance eduction method is used to educe the impedance for each segment of the two-segment liner, and the results are compared with those educed for the uniform liners. These impedance spectra are shown to compare favorably for the majority of test conditions. Poorer comparisons are achieved for those test conditions where one segment of the two-segment liner provides little attenuation. Poor attenuation is a wellknown cause for impedance eduction difficulties. Overall, this multisegment impedance eduction method offers the potential to study complicated liners in a more efficient manner (i.e., without the requirement to build and test separate liners to duplicate each unique segment of the multisegment liner). More detailed studies are required to further validate this tool, and are intended to be the focus of future research.
    Keywords: Acoustics
    Type: NF1676L-28585 , AIAA Aviation Forum; Jun 25, 2018 - Jun 29, 2018; Atlanta, GA; United States
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  • 8
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    Applications of Parallel-Element, Embedded Mesh-Cap Acoustic Liner Concepts (2018)
    In:  CASI
    Details
    Publication Date: 2019-07-20
    Description: This study explores progress achieved with 2DOF, 3DOF, and MDOF acoustic liners constructed with mesh caps embedded within a honeycomb core. These liner configurations offer potential for broadband noise reduction, and are suitable for conventional aircraft implementation. Samples for each configuration are tested in the NASA normal incidence tube and grazing flow impedance tube, with and without a wire mesh facesheet. Impedances based on these measured data compare favorably with those predicted using a transmission line impedance prediction model. Predicted impedances are then used as input for an aeroacoustic propagation code to compute axial acoustic pressure distributions in the grazing flow tube. These predicted distributions compare favorably with the corresponding measured distributions at frequencies away from the frequency of peak attenuation, but suffer slight degradation for frequencies very near the peak attenuation frequency, where the predicted results are sensitive to input impedance changes. As expected, the noise reduction frequency range increases as more degrees of freedom are included. Although the specific results achieved herein may differ from those that would be achieved with other 2DOF, 3DOF, and MDOF liners, this comparison highlights some of the key features that can be exploited in the design of parallel-element, embedded mesh-cap liners.
    Keywords: Acoustics
    Type: NF1676L-28554 , AIAA Aviation Forum 2018; Jun 25, 2018 - Jun 29, 2018; Atlanta, GA; United States
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  • 9
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    A Method for Optimizing Non-Axisymmetric Liners for Multimodal Sound Sources (2002)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: Central processor unit times and memory requirements for a commonly used solver are compared to that of a state-of-the-art, parallel, sparse solver. The sparse solver is then used in conjunction with three constrained optimization methodologies to assess the relative merits of non-axisymmetric versus axisymmetric liner concepts for improving liner acoustic suppression. This assessment is performed with a multimodal noise source (with equal mode amplitudes and phases) in a finite-length rectangular duct without flow. The sparse solver is found to reduce memory requirements by a factor of five and central processing time by a factor of eleven when compared with the commonly used solver. Results show that the optimum impedance of the uniform liner is dominated by the least attenuated mode, whose attenuation is maximized by the Cremer optimum impedance. An optimized, four-segmented liner with impedance segments in a checkerboard arrangement is found to be inferior to an optimized spanwise segmented liner. This optimized spanwise segmented liner is shown to attenuate substantially more sound than the optimized uniform liner and tends to be more effective at the higher frequencies. The most important result of this study is the discovery that when optimized, a spanwise segmented liner with two segments gives attenuations equal to or substantially greater than an optimized axially segmented liner with the same number of segments.
    Keywords: Acoustics
    Type: AIAA Paper 2002-2516 , 8th AIAA/CEAS Aeroacoustics Conference; Jun 17, 2002 - Jun 19, 2002; Breckenridge, CO; United States
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  • 10
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    Comparison of Acoustic Impedance Eduction Techniques for Locally-Reacting Liners (2003)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: Typical acoustic liners used in current aircraft inlets and aft-fan ducts consist of some type of perforated facesheet bonded to a honeycomb core. A number of techniques for determining the acoustic impedance of these locallyreacting liners have been developed over the last five decades. In addition, a number of models have been developed to predict the acoustic impedance of locallyreacting liners in the presence of grazing flow, and to use that information together with aeroacoustic propagation codes to assess the noise absorption provided by these liners. These prediction models have incorporated the results from databases acquired with specific impedance eduction techniques. Thus, while these prediction models are acceptable for liners that are similar to those tested in these databases, their application to new liner configurations must be viewed with caution. The primary purpose of this paper is to provide a comparison of impedance eduction techniques that have been implemented at various aerospace research laboratories in the United States (NASA Langley Research Center, General Electric Aircraft Engines, B. F. Goodrich and Boeing). A secondary purpose is to provide data for liner configurations that extend the porosity range beyond that which has been previously used in common aircraft engine nacelles. Two sets of liners were designed to study the effects of three parameters: perforate hole diameter, facesheet thickness and porosity. These two sets of liners were constructed for testing in each of the laboratories listed above. The first set of liners was designed to fit into the NASA Langley and Boeing test facilities. The second set was designed to fit into the General Electric Aircraft Engines and B. F. Goodrich test facilities. By using the same parent material, both sets of liners were identical to within the limits of material and fabrication variability. Baseline data were obtained in the normal incidence impedance tubes at NASA Langley and B. F. Goodrich. The results were found to compare extremely well. The samples were then tested in the grazing flow ducts of each of the four laboratories. Perhaps the most significant result of these comparisons is that the educed acoustic resistances for the liners used in this study increase as the mean flow profile is modified from uniform to 3-D shear. This realization has demonstrated the need for an frequency-dependent impedance eduction technique that incorporates 3-D shear flow and is efficient.
    Keywords: Acoustics
    Type: AIAA Paper 2003-3306 , 9th AIAA/CEAS Aeroacoustics Conference and Exhibit; May 12, 2003 - May 14, 2003; Hilton Head, SC; United States
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