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  • 1
    Electronic Resource
    Electronic Resource
    Hotspots of homologous recombination (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 234-241 
    Details
    ISSN: 1420-9071
    Keywords: Homologous recombination ; hotspots ; nucleases ; meiosis ; Escherichia coli ; Chi ; Schizosaccharomyces pombe ; M26
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Homologous recombination occurs at higher than average frequency at and near hotspots. Hotspots are special nucleotide sequences recognized by proteins that promote, directly or indirectly, a rate limiting step of recombination. This review focuses on two well-studied examples, the Chi sites of the bacteriumEscherichia coli and the M26 site of the fission yeastSchizosaccharomyces pombe. Chi, 5′ G-C-T-G-G-T-G-G 3′, is recognized by the RecBCD enzyme, which nicks the DNA near Chi and produces a 3′-ended single-stranded DNA ‘tail’; this tail is a potent substrate for homologous pairing by RecA and single-stranded DNA binding proteins. M26, 5′ A-T-G-A-C-G-T 3′, is recognized by a heterodimeric protein and stimulates, by an as-yet-unknown mechanism, meiotic recombination at and near theade6 gene. Additional hotspots in bacteria, fungi, and mammals enhance recombination directly or indirectly via a variety of mechanisms. Although hotspots are widespread among organisms, the biological role of their localized enhancement of recombination remains a matter of speculation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01924006
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  • 2
    Electronic Resource
    Electronic Resource
    Nitrogen transfer from arrowleaf clover to ryegrass in field plantings (1990)
    Springer
    Plant and soil 128 (1990), S. 293-297 
    Details
    ISSN: 1573-5036
    Keywords: arrowleaf clover ; labelled N ; Lolium multiflorum ; 15N depleted ammonium nitrate ; N transfer ; ryegrass ; Trifolium vesiculosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Arrowleaf clover (Trifolium vesiculosum Savi) and annual ryegrass Lolium multiflorum Lam.) commonly are overseeded in dormant bermudagrass (Cynodon dactylon L. Pers.) sod on coastal plain soils in the southeastern United States. Two field experiments were conducted in consecutive years at different sites to estimate the amount of N transferred from the clover to the annual grass. Nitrogen treatments included 50 kg N ha-1 as 15N depleted ammonium nitrate applied in either February or April, and a check (no N applied). Three clippings were made during the cool-season from March to June. In both experiments, less than 5 kg N ha-1 were transferred from the clover to the grass. Ryegrass yields of dry matter and total N were not increased by growing with clover. Clover growth was typical for the region; average dry matter yield in pure stand was 2,615 kg ha-1 over the two-year period. Clover in mixed stand fixed between 20 and 60 kg N/ha. Less than 13% of N contained in ryegrass was transferred from arrowleaf clover to ryegrass at any clipping while clover was actively growing. The quantity of N transferred over the entire season was not statistically significant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00011122
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    Paper (German National Licenses)
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