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  • Articles  (10)
  • 04. Solid Earth::04.08. Volcanology::04.08.06. Volcano monitoring  (6)
  • Cell & Developmental Biology  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Prostaglandin-stimulated second messenger signaling in bone-derived endothelial cells is dependent on confluency in culture (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 160 (1994), S. 585-595 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: New bone formation is associated with an increase in blood flow by the invasion of capillaries. Endothelial cells that line the capillaries can produce paracrine factors that affect bone growth and development, and in turn, could be affected by products produced by bone cells, in particular the osteoblasts. Since osteoblasts produce prostaglandins E2 and F2α (PGE2, PGF2α), it was investigated if these PGs were agonists to bone-derived endothelial cells (BBE) by assessing changes in cAMP and free cytosolic calcium concentration ([Ca2+]i) second messenger generation. We found that confluent cultures of BBE cells, a clonal endothelial cell line derived from bovine sternal bone, responded to 1 μM PGE2 by an increase in cAMP. PGF2α at the same concentration was less potent in stimulating an increase in cAMP production in confluent BBE cells. Subconfluent cells with a morphology similar to that of fibroblastic cells were not as sensitive to PGE2-stimulated cAMP generation. PGF2α failed to elicit any cAMP production in subconfluent cultures. PGE2 and PGF2α both stimulated an increase in [Ca2+]i concentration in a dose-dependent manner. The potency of PGE2 was similar to that of PGF2α in stimulating an increase in [Ca2+]i. The Ca2+ response was mostly independent of extracellular Ca+, was unchanged even with prior indomethacin treatment, was unaffected by caffeine pretreatment, but was abolished subsequent to thapsigargin pretreatment. The PG-induced increase in [Ca2+]i was also dependent on the confluency of the cells. In a subconfluent state, the responses to PGE2 or PGF2α were either negligible, or only small increases in [Ca2+]i were noted with high concentrations of these two PGs. Consistent, dose-dependent increases in [Ca2+]i were stimulated by these PGs only when the cells were confluent and had a cobblestoned appearance. Since it was previously demonstrated that BBE cells respond to parathyroid hormone (PTH) by the production of cAMP, we tested if bovine PTH(1-34) amide bPTH(1 - 34) also increased [Ca2+]i in these cells. No change in [Ca2+]i was found in response to bPTH (1 - 34), although bPTH (1 - 34) stimulated a nine to tenfold increase in cAMP. We conclude that BBE cells respond to PGE2 and PGF2α but not to bPTH(1 - 34) by an increase in [Ca2+]i probably secondary to stimulation of phospholipase C and that the cAMP and [Ca2+]i second messenger responses in BBE cells are dependent on the state of confluency of the cells. © 1994 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041600322
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  • 2
    Electronic Resource
    Electronic Resource
    In vitro structural and functional relationships between preosteoclastic and bone endothelial cells: A juxtacrine model for migration and adhesion of osteoclast precursors (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 162 (1995), S. 199-212 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The role of vascularization in the process of bone resorption has not been clarified. The interactions between vascular endothelium and osteoclast progenitors were analyzed using clonal cell lines of bone-derived endothelial and preosteoclastic cells. Insulin-like growth factor I is a major chemotactic stimulator of preosteoclastic cell migration mediated by bone endothelial cells. Osteoclast precursors rapidly adhered to bone endothelial monolayers. This phenomenon appeared to be cell-specific and mediated through the binding of vitronectin and fibronection receptors to fibronectin. In addition, direct contact with bone endothelial cells induced osteoclast progenitors to differentiate into more mature elements, with the tendency to cluster together to form large multinucleated cells. These findings demonstrated specific in vitro interactions between bone endothelial cells and osteoclast progenitors, offering a new model for understanding the molecular mechanisms which direct the processes of osteoclast recruitment and ontogeny. © 1995 Wiley-Liss, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041620206
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  • 3
    Electronic Resource
    Electronic Resource
    Role for autocrine TGF-β1 in regulating differentiation of a human leukemic cell line toward osteoclast-like cells (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 160 (1994), S. 482-490 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Increasing evidence suggests that transforming growth factor-β (TGF-β) is involved in bone formation during remodeling. Using a recently cloned human leukemic cell line (FLG 29.1 cells) we demonstrate that these cells synthesize and secrete TGF-β1 and that exogenous or autocrine TGF-β1 can induce the same features of osteoclastic-like cells, exerting its effects through the binding to TGF-β specific receptors. Scatchard analysis of 125I-labeled TGF-β1 to FLG 29.1 cells revealed the presence of a single high affinity binding site with a Kd value of ∼25 pM and a binding capacity of ∼900 sites/cell. Affinity labeling experiments showed that FLG 29.1 cells express type I and type II TGF-β receptors. Stimulation of FLG 29.1 cells with low TGF-β1 doses reduced cell proliferation and increased cell adhesion and tartrate resistant acid phosphatase (TRAcP) activity. Pretreatment of FLG 29.1 cells with TGF-β1 caused a significant and dose-dependent response to calcitonin. Northern blot of total mRNA and analysis of the conditioned media (CM) showed that TGF-β1 was synthesized by FLG 29.1 cells. TPA treatment, which induces partial differentiation of these cells, markedly increased TGF-β1 mRNA expression and growth factor release. The majority of TGF-β1 secreted by TPA-treated cells was in its latent form. However, anti-TGF-β antibodies inhibited TGF-β1 and TPA-induced growth inhibition, calcitonin responsiveness, and TRAcP activity, suggesting that the TPA effect is mediated in part by autocrine TGF-β1 and indicating that the cells can activate and respond to the TGF-β that they secrete. These findings support a potential autocrine role for TGF-β1 in osteoclast differentiation. © 1994 Wiley-Liss, Inc.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041600311
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  • 4
    Electronic Resource
    Electronic Resource
    Apoptosis in C3H-10T1/2 cells: Roles of intracellular pH, protein kinase C, and the Na+/H+ antiporter (1997)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 67 (1997), S. 231-240 
    Details
    ISSN: 0730-2312
    Keywords: 5-(N, N-hexamethylene)-amiloride (HMA) ; 12-O-tetradecanoylphorbol-13-acetate (TPA) ; chelerythrine ; protein kinase C (PKC) ; serum withdrawal ; internucleosomal DNA cleavage ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Changes in intracellular ion concentrations have been correlated with the activation of an endogenous endonuclease and thus internucleosomal DNA cleavage during apoptosis in many cell types. We investigated whether intracellular pH could play a significant role in apoptotic initiation and progression in C3H-10T1/2 cells, a cell strain that does not exhibit double-stranded DNA cleavage during apoptosis. Protein kinase C and the Na+/H+ antiporter, known regulators of intracellular pH, also were assessed for their involvement in apoptosis of C3H-10T1/2 cells. When a H+ ionophore was used to clamp intracellular pH to 6.0 or below, a significant level of apoptosis was induced in these cells within 6 h, whereas clamping at pH 6.75 did not induce significant amounts of apoptosis until 36 h after acidification. The acidified cells exhibited classic apoptotic morphology and chromatin condensation, similar to serum withdrawn cells, but failed to show internucleosomal DNA cleavage with electrophoresis of genomic DNA. Our results also suggest that the 12-O-tetradecanoylphorbol-13-acetate (TPA)-mediated inhibition of apoptosis in serum withdrawn C3H-10T1/2 cells functions through a sequential activation of protein kinase C and the Na+/H+ antiporter; thus, an alkalinization or an inhibition of acidification is involved in this apoptotic block. Serum withdrawal itself does not appear to act through a negative effect on either protein kinase C or the Na+/H+ antiporter. TPA was also capable of inhibiting the apoptosis induced by specific inhibitors of protein kinase C and the Na+/H+ antiporter, but the inhibition was successful only if the TPA was administered at least 20 min prior to the addition of the enzyme inhibitor. These results indicate that apoptosis in C3H-10T1/2 cells follows a pathway that involves intracellular acidification, but is independent of detectable endonuclease activity. J. Cell. Biochem. 67:231-240, 1997. © 1997 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
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  • 5
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    AREA VULCANICA NAPOLETANA: 10 ANNI DI OSSERVAZIONI GPS (2007)
    Details
    Publication Date: 2017-04-04
    Description: L’area napoletana è una delle zone a più alto rischio vulcanico, per la presenza di tre strutture vulcaniche attive (il Somma-Vesuvio, la caldera dei Campi Flegrei e l’Isola d’Ischia) e per l’intensa urbanizzazione della zona. La concentrazione dei suddetti vulcani attivi in un’area molto urbanizzata rende fondamentale la presenza di un sistema di monitoraggio che registri i fenomeni connessi al processo vulcanico in atto e che dia informazioni utili per modellarne il comportamento. Tra i vari fenomeni che generalmente sono associati ai processi vulcanici, le deformazioni statiche giocano un ruolo importante per lo studio dei parametri delle sorgenti magmatiche e per la loro modellazione. Nell’area vulcanica napoletana è presente una estesa rete GPS (sia permanente che discreta) che negli ultimi 10 anni ha permesso di raccogliere una mole importante di dati e di avere informazioni circa la dinamica in atto. I dati raccolti in tale periodo, a causa dei rapidi sviluppi della tecnologia GPS, della strumentazione, delle metodologie di processamento, presentavano alcune disomogeneità nella qualità, nell’archiviazione e nell’elaborazione. Pertanto è stato necessario un notevole lavoro di verifica, correzione ed omogeneizzazione dal punto di vista qualitativo di tutti i dati disponibili. Successivamente si è potuto procedere al riprocessamento dei dati, utilizzando il software GPS Bernese v. 5.0, secondo i più recenti standard IGS. Questo lavoro ha permesso di creare un database accurato di tutti i dati GPS disponibili per l’intera area vulcanica napoletana e il conseguente miglioramento della qualità dei risultati ha fornito un utile contributo all’interpretazione dei movimenti del suolo avvenuti negli ultimi anni. In particolare, i nuovi dati ottenuti hanno permesso un’accurata descrizione dell’andamento temporale dei modesti fenomeni di sollevamento (mini-uplift) che hanno interessato la caldera flegrea negli ultimi anni.
    Description: Published
    Description: Centro Congressi Lingotto, Torino
    Description: open
    Keywords: area napoletana ; osservazioni GPS ; 04. Solid Earth::04.03. Geodesy::04.03.06. Measurements and monitoring ; 04. Solid Earth::04.08. Volcanology::04.08.06. Volcano monitoring ; 05. General::05.01. Computational geophysics::05.01.01. Data processing
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: Conference paper
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  • 6
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    Monitoraggio del Vesuvio con antenne sismiche: primi risultati dell’array VAS (2015)
    Details
    Publication Date: 2017-04-04
    Description: Nei primi mesi del 2012 è stato installato, sul versante sud del Vesuvio, un array di sismometri, denominato VAS (Vesuvius Array South) con lo scopo di migliorare il monitoraggio dell'attività sismica del vulcano. L'array è composto da 10 sensori a corto periodo, di cui uno a tre componenti, per un totale di 12 canali acquisiti da due stazioni sismiche (ciascuna a 6 canali), che operano in modalità continua su supporto locale con frequenza di campionamento di 100 campioni/secondo e range dinamico di 24 bit. I dati, prelevati periodicamente, sono elaborati con quattro diverse tecniche di array in varie bande di frequenza nell'intervallo 1 – 5 Hz al fine di evidenziare ogni segnale potenzialmente legato all'attività vulcanica. I risultati preliminari dell'analisi di terremoti locali mostrano che l'array ha una buona risoluzione in termini di slowness e backazimuth nella banda di frequenza 2 – 5 Hz. A frequenza maggiore i risultati possono essere affetti da errori di elevata entità, mentre a frequenza minore la coerenza del rumore può essere equivalente a quella del segnale prodotto dai terremoti, e quindi mascherare l'individuazione di eventi naturali potenzialmente legati a variazioni nella dinamica del vulcano.
    Description: Published
    Description: 1-18
    Description: 5V. Sorveglianza vulcanica ed emergenze
    Description: N/A or not JCR
    Description: open
    Keywords: array sismico ; sismicità vulcanica ; 04. Solid Earth::04.08. Volcanology::04.08.06. Volcano monitoring
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 7
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    Somma Vesuvius volcano: ground deformations from CGPS observations (2001-2012) (2015)
    INGV, Istituto Nazionale di Geofisica e Vulcanologia
    Details
    Publication Date: 2017-04-04
    Description: This paper is a contribution to the evaluation of ground deformations at Somma-Vesuvius volcano by means GPS measurements from 2001 to 2012. In this study we use a dataset from nine continuous GPS stations of the Neapolitan Volcanoes Continuous GPS network (NeVoCGPS), which covers the Neapolitan volcanic area, and is operated by the Istituto Nazionale di Geofisica e Vulcanologia. The GPS data processing is performed by the Bernese software v. 5.0. The results of the data processing show that the dynamics of the Somma-Vesuvio volcano, between 2001 and 2012, is characterized by a general subsidence, with maximum values on the Gran Cono at BKNO (−11.7 ± 0.65 mm/year) and BKE1 (−4.92 ± 0.36 mm/year) stations. The subsidence decrease from the crater down to the coast and the horizontal displacements are concentrated in Gran Cono area, the youngest part of the volcano. The parameters of the principal strain components indicate that Somma-Vesuvius is affected by a predominant contraction phase, which is concentrated in the areas with the greatest altitudes.
    Description: Published
    Description: S0456
    Description: 2V. Dinamiche di unrest e scenari pre-eruttivi
    Description: JCR Journal
    Description: open
    Keywords: Ground deformation, CGPS, Somma-Vesuvius. ; 04. Solid Earth::04.08. Volcanology::04.08.06. Volcano monitoring
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
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  • 8
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    NeVoCGPS network: contributions to the Deformation Analysis in Neapolitan Volcanic area based on Continuous GPS measurements (2012)
    Details
    Publication Date: 2017-04-04
    Description: Osservatorio Vesuviano, department of Istituto Nazionale di Geofisica e Vulcanologia, installed a permanent GPS network (NeVoCGPS), constituted of 25 stations, in Neapolitan volcanic area, where three active volcanoes (Somma-Vesuvius, Campi Flegrei caldera and Ischia Island) rise, each characterized by a peculiar type of ground movements activity. The Somma-Vesuvius system exhibits now a low level of ground deformation; the Campi Flegrei, caracterized from over 2000 years by slow up and down vertical movements (bradyseism), at present is in a very slow uplift phase; Ischia, finally, shows subsidence in the specific areas (Southern and North-West sectors of the island). The presence of these volcanoes and the dense urbanization of the area make the ground deformation monitoring a crucial point for risk mitigation and modelling aims. The 3D ground displacements are calculated using CGPS data, acquired with a 30s rate and with the daily and weekly vertexes position estimate. All the stations are managed by remote control and the data are automatically downloaded and processed using Bernese software package. The entire chain of acquisition, processing and data analysis is accurately described and some results obtained in the last years are shown.
    Description: Published
    Description: held 19-24 April, 2009 in Vienna, Austria http://meetings.copernicus.org/egu2009
    Description: 1.3. TTC - Sorveglianza geodetica delle aree vulcaniche attive
    Description: 1.9. Rete GPS nazionale
    Description: open
    Keywords: permanent GPS network ; NeVoCGPS ; Somma-Vesuvius, Campi Flegrei caldera and Ischia Island ; ground movements activity ; 04. Solid Earth::04.03. Geodesy::04.03.07. Satellite geodesy ; 04. Solid Earth::04.08. Volcanology::04.08.06. Volcano monitoring ; 05. General::05.09. Miscellaneous::05.09.99. General or miscellaneous
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: Conference paper
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  • 9
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    CAMPAGNA DI MISURE GPS ALL’ISOLA D’ISCHIA (MAGGIO-GIUGNO 2010) (2011)
    Details
    Publication Date: 2017-04-04
    Description: L’Osservatorio Vesuviano, Sezione di Napoli dell’Istituto Nazionale di Geofisica e Vulcanologia, gestisce il sistema di monitoraggio per la sorveglianza delle aree vulcaniche del Somma Vesuvio, dei Campi Flegrei e dell’isola d’Ischia. Tale sistema comprende reti geofisiche e geochimiche: quelle geofisiche controllano l’attività sismica, le deformazioni del suolo e le variazioni del campo gravimetrico, mentre quelle geochimiche misurano la composizione chimica dei gas emessi dalle fumarole e di quelli disciolti nelle acque di falda. Per quanto concerne l’isola d’Ischia, il controllo delle deformazioni del suolo viene effettuato utilizzando tecniche classiche (livellazioni di precisione, gravimetria, mareometria) e metodi satellitari (GPS, SAR). In particolare, sull’isola sono state installate una rete di livellazione, una rete gravimetrica, un mareografo e una rete GPS. Nel corso del primo semestre del 2010, sull’isola d’Ischia sono state effettuate una campagna gravimetrica, una di livellazione ed una di misure GPS. Oggetto di questo rapporto è la descrizione dell’attività svolta durante la campagna di misure GPS realizzata dal 28 maggio (DoY 148) al 11 giugno (DoY 162) 2010.
    Description: Istituto Nazionale di Geofisica e Vulcanologia
    Description: Published
    Description: 1.3. TTC - Sorveglianza geodetica delle aree vulcaniche attive
    Description: open
    Keywords: misure GPS ; Ischia ; 04. Solid Earth::04.03. Geodesy::04.03.06. Measurements and monitoring ; 04. Solid Earth::04.08. Volcanology::04.08.06. Volcano monitoring
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: report
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  • 10
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    GPS Monitoring at Vesuvio, Campi Flegrei Caldera and Ischia Island (Southern Italy) (2011)
    Details
    Publication Date: 2017-04-04
    Description: The Neapolitan volcanic area is located in the southern part of the Campanian plain and includes three active volcanoes (Vesuvius, Campi Flegrei Caldera and Ischia Island). This area shows different dynamical behaviours. Campi Flegrei caldera represents one well known and peculiar example of ground deformations (bradyseism), with periods of intense uplift during the 1969-72 and 1982-84, followed by subsidence phase with some episodic mini-uplifts superimposed. Contrary, Vesuvius is a substantially stable volcano, with small and localized subsidence mainly in the crater zone. Ischia Island has been characterized by subsidence in the S and NW sectors of the island. The presence of these three volcanoes in a dense populated area, makes ground deformation detection a crucial point in the risk mitigation. Ground deformation is an important volcanic precursor, because linked to magma overpressure and migration, thus, continuous monitoring and modelling is one of the main instruments to attempt for a short time forecast of eruptive activity. Since several years, the INGV – Osservatorio Vesuviano installed a permanent GPS network (NeVoCGPS), constituted of 27 stations, in the Neapolitan volcanic area with a configuration that guarantees a continuous and fast 3D information about the dynamics of the area. All the GPS stations are managed by remote control, the data are daily downloaded automatically. After an automatic quality control procedure, the data processing is performed by the Bernese Processing Engine (BPE) of the Bernese GPS software v. 5.0. In this work, the entire chain of data acquisition and processing is described and some results obtained in last years are presented.
    Description: INGV,Sezione di Napoli,Osservatorio Vesuviano
    Description: Published
    Description: Puerto de la Cruz, Tenerife (Canary Islands, Spain)
    Description: open
    Keywords: GPS Monitoring ; Vesuvio ; Campi Flegrei Caldera ; Ischia Island ; 04. Solid Earth::04.03. Geodesy::04.03.06. Measurements and monitoring ; 04. Solid Earth::04.08. Volcanology::04.08.06. Volcano monitoring
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: Poster session
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