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  • 1
    Publication Date: 1998-02-07
    Description: In order to reach the inner membrane of the mitochondrion, multispanning carrier proteins must cross the aqueous intermembrane space. Two essential proteins of that space, Tim10p and Tim12p, were shown to mediate import of multispanning carriers into the inner membrane. Both proteins formed a complex with the inner membrane protein Tim22p. Tim10p readily dissociated from the complex and was required to transport carrier precursors across the outer membrane; Tim12p was firmly bound to Tim22p and mediated the insertion of carriers into the inner membrane. Neither protein was required for protein import into the other mitochondrial compartments. Both proteins may function as intermembrane space chaperones for the highly insoluble carrier proteins.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Koehler, C M -- Jarosch, E -- Tokatlidis, K -- Schmid, K -- Schweyen, R J -- Schatz, G -- New York, N.Y. -- Science. 1998 Jan 16;279(5349):369-73.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9430585" target="_blank"〉PubMed〈/a〉
    Keywords: Biological Transport ; Carrier Proteins/*metabolism ; Cloning, Molecular ; Fungal Proteins/genetics/*metabolism ; Genes, Fungal ; Hot Temperature ; Intracellular Membranes/*metabolism ; Membrane Potentials ; Membrane Proteins/genetics/*metabolism ; *Membrane Transport Proteins ; Mitochondria/*metabolism ; Mitochondrial ADP, ATP Translocases/metabolism ; Mitochondrial Membrane Transport Proteins ; Models, Biological ; Molecular Chaperones/metabolism ; Mutagenesis ; Phosphate-Binding Proteins ; Saccharomyces cerevisiae/genetics/metabolism ; *Saccharomyces cerevisiae Proteins ; Solubility
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 164 (1989), S. 285-322 
    ISSN: 1615-6110
    Keywords: Algae ; Desmidiaceae ; Micrasterias ; Lateral motion ; microtubules ; microfilaments ; secondary wall microfibrils ; preprophase band ; nucleus position ; septum ; morphogenesis ; membrane flows ; chromatidal repulsion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract On the basis of the “filament rotation model” that was elaborated for interpretations in cell motility, the lateral hydrodynamic effects of rotating filaments have been investigated by large-scale model experiments. Helices were rotated by small electric motors in a medium of high viscosity (honey or polyethyleneglycol). The observed effects, hitherto not investigated in detail by hydrodynamics, show some features that were attributed to the indefinable “formative power” or “vital force” of the past. The main effects generated by the rotating filaments are (1) flows and flow patterns with “impact zones” where flows collide, (2) regions of excessive pressure and negative pressure (“corner effect”) along a wall, (3) grooves and smoothly shaped ridges on a free fluid surface, and (4) “rolling” motions of freely hanging filaments. All effects and flow patterns depend on the appropriate distribution of rotating and counterrotating filaments. Each change of the rotational direction means a dramatic alteration. The application of the observed effects explains largely the function of the microtubule/microfilament hoops or helices during the cytokinesis of a plant cell. Interpretations or simulations are described for events as the formation of secondary wall thickenings, the orientation of their microfibrils, the motion of the preprophase band microtubules, the formation of the phragmosome, the migration, stationary position and shape of the preprophase nucleus, the girdle-, septum- and H-piece formation of cell walls in algae and some events of morphogenesis inMicrasterias. Further interpretations are related to the lateral flows and to invaginations of free cell membranes, to lateral filament motions, to the “right-left problem”, to the selfintertwining of filaments, to the rotation of a cell body by its flagellum, to the repulsion of chromatids during meiosis and to the tetragonal and hexagonal arrangement of filaments.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1615-6102
    Keywords: Filaments-rotation ; Microtubules ; Microfilaments ; Cell wall microfibrils ; Cytomorphogenesis ; Patterning ; Preprophase ; Septum ; Micrasterias
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rotating filaments produce far reaching lateral streams in a thick medium and localized negative pressure when placed adjacent a wall. Freely movable filaments can roll on a wall. Pairs of counterrotating filaments are stabilized. When rotating components of the cytoskeleton generate these lateral hydrodynamic effects many hitherto mysterious features can be explained, including positioning of organelles and morphogenesis of plant cells. It is postulated that MTs and MFs roll laterally to positions of equilibrium, these being, for example, the preprophase band site and the cortical site that controls local thickening of the secondary wall. The orientation of microfibrils in the cell wall may also depend on the lateral effects of rotation. Different streaming patterns can move and shape the nucleus and other organelles and bring them in appropriate positions. Morphogenetic events as septum and lobe formation in desmids could result. Time-dependent reversal of the rotational directions are required for the transformation of the patterns.
    Type of Medium: Electronic Resource
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