Publication Date:
1985-11-15
Description:
A biphasic plasmid vector was used to introduce the Escherichia coli K-12 lac operon into the unicellular cyanobacterium Agmenellum quadruplicatum PR-6. The PR-6 transformants expressed beta-galactosidase at nearly as high a level as did Escherichia coli transformants. In order to accomplish this, it was necessary to obtain PR-6 mutants that could be transformed by plasmids with unmodified recognition sites for the endogenous PR-6 restriction endonuclease Aqu I. These mutants were generated by a variation of the ectopic mutagenesis techniques that have been used in other naturally transforming bacteria. The ability to assay the expression of lacZ in PR-6 paves the way for the construction of gene fusions with various PR-6 promoters and quantitation of their expression under specific in vivo conditions.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Buzby, J S -- Porter, R D -- Stevens, S E Jr -- GM 28609/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1985 Nov 15;230(4727):805-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2997920" target="_blank"〉PubMed〈/a〉
Keywords:
Cyanobacteria/*genetics
;
DNA Restriction Enzymes
;
Escherichia coli/*genetics
;
*Genetic Vectors
;
*Lac Operon
;
*Plasmids
;
beta-Galactosidase/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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