Publication Date:
1993-07-09
Description:
Direct gene transfer into adult animals resulting in generalized or tissue-specific expression would facilitate rapid analysis of transgene effects and allow precise in vivo manipulation of biologic processes at the molecular level. A single intravenous injection of expression plasmid:cationic liposome complexes into adult mice efficiently transfected virtually all tissues. In addition to vascular endothelial cells, most of the extravascular parenchymal cells present in many tissues including the lung, spleen, lymph nodes, and bone marrow expressed the transgene without any apparent treatment-related toxicity. The transgene was still expressed in large numbers of cells in multiple tissues for at least 9 weeks after a single injection. Expression could be targeted to specific tissues and cell types, depending on the promoter element used.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhu, N -- Liggitt, D -- Liu, Y -- Debs, R -- New York, N.Y. -- Science. 1993 Jul 9;261(5118):209-11.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cancer Research Institute, University of California, San Francisco 94143-0128.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7687073" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Base Sequence
;
Bone Marrow/metabolism
;
Chloramphenicol O-Acetyltransferase/genetics
;
Cystic Fibrosis/genetics
;
Cystic Fibrosis Transmembrane Conductance Regulator
;
Cytomegalovirus/genetics
;
Female
;
*Gene Expression
;
Injections, Intravenous
;
Liposomes
;
Liver/metabolism
;
Lung/metabolism
;
Lung Neoplasms/genetics
;
Lymphoid Tissue/metabolism
;
Membrane Proteins/genetics
;
Mice
;
Mice, Inbred ICR
;
Mice, Transgenic
;
Molecular Sequence Data
;
Myocardium/metabolism
;
Oligodeoxyribonucleotides
;
Phosphatidylethanolamines/chemistry
;
Plasmids
;
Quaternary Ammonium Compounds
;
*Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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