Publication Date:
2009-02-14
Description:
Infection by human rhinovirus (HRV) is a major cause of upper and lower respiratory tract disease worldwide and displays considerable phenotypic variation. We examined diversity by completing the genome sequences for all known serotypes (n = 99). Superimposition of capsid crystal structure and optimal-energy RNA configurations established alignments and phylogeny. These revealed conserved motifs; clade-specific diversity, including a potential newly identified species (HRV-D); mutations in field isolates; and recombination. In analogy with poliovirus, a hypervariable 5' untranslated region tract may affect virulence. A configuration consistent with nonscanning internal ribosome entry was found in all HRVs and may account for rapid translation. The data density from complete sequences of the reference HRVs provided high resolution for this degree of modeling and serves as a platform for full genome-based epidemiologic studies and antiviral or vaccine development.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923423/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923423/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Palmenberg, Ann C -- Spiro, David -- Kuzmickas, Ryan -- Wang, Shiliang -- Djikeng, Appolinaire -- Rathe, Jennifer A -- Fraser-Liggett, Claire M -- Liggett, Stephen B -- R01 HL091490/HL/NHLBI NIH HHS/ -- U19 AI070503/AI/NIAID NIH HHS/ -- U19-AI070503/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2009 Apr 3;324(5923):55-9. doi: 10.1126/science.1165557. Epub 2009 Feb 12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Molecular Virology, University of Wisconsin, Madison, WI 53706, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19213880" target="_blank"〉PubMed〈/a〉
Keywords:
3' Untranslated Regions
;
5' Untranslated Regions
;
Base Composition
;
Base Sequence
;
Codon, Terminator
;
*Evolution, Molecular
;
*Genome, Viral
;
Humans
;
Molecular Sequence Data
;
Mutation
;
Nucleic Acid Conformation
;
Open Reading Frames
;
Phylogeny
;
Picornaviridae Infections/virology
;
Polyproteins/biosynthesis/chemistry/genetics
;
RNA, Viral/chemistry/*genetics
;
Recombination, Genetic
;
Respiratory Tract Infections/virology
;
Rhinovirus/classification/*genetics/ultrastructure
;
Sequence Alignment
;
Sequence Analysis, RNA
;
Serotyping
;
Viral Proteins/biosynthesis/chemistry/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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